Isolation of protoplasts from fern prothallia and their regeneration to gametophytes

  • Masuo Maeda
  • Michio Ito


Protoplasts were isolated enzymatically from prothallia ofLygodium japonicum. The protoplasts grown in a culture medium containing 0.6 M mannitol and 0.05 M sucrose began to divide within 8 days of culture, and after 30 days 10-cell clusters were present. When the cell-clusters were transferred into fresh media followed by sequential reduction of mannitol concentration, they developed rhizoids and protonemata. The reduction of mannitol concentration to 0.3 M resulted in the regeneration of a common gametophyte within 50 days of culture, and subsequently the regenerated gametophytes produced sporophytic leaves and roots.

Key words

Fern gametophyte Lygodium Prothallium Protoplast Regeneration 


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Copyright information

© The Botanical Society of Japan 1981

Authors and Affiliations

  • Masuo Maeda
    • 1
    • 2
  • Michio Ito
    • 1
    • 2
  1. 1.Biological Laboratory, Faculty of EducationFukui UniversityFukui
  2. 2.Department of Biology, Faculty of ScienceNagoya UniversityNagoya

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