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Abnormal flower formation of tobacco plants regenerated from callus cultures

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Abstract

The plantlets regenerated from tobacco calluses, subcultured for prolonged periods, were weak and generally could not be cultivated into mature flowering specimens. However, some of them flowered and various kinds of abnormal flowers as well as leaves were observed in all the tobacco plants bearing flowers. They were sterile at had abnormal immature pollen grains. Some of them occasionally germinated in the anther. Abnormalities were also found in the nucleus of immmature pollen grains. Such drastic floral abnormalities were not found in tobacco plants derived from seeds and callus cultures subcultured for a relatively short period under the same conditions of cultivation.

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Abbreviations

IAA:

3-indolylycetic acid

References

  • Audus, L.J. 1963. Plant Growth Substances. Leonard Hill Ltd., London.

    Google Scholar 

  • Gautheret, R.J. 1955. Sur la variabilité des propriétés physiologiques des cultures de tissues végétaux. Ann. Biol.31: 345–371.

    Google Scholar 

  • Ishihara, A. 1965. On the growth and organ formation of callus tissue in vitro originated from carrot roots (in Japanese). Proc. Crop Sci. Soc. Japan16: 225–233.

    Google Scholar 

  • Melchers, G. 1965. Einige genetisch Gesichtspunkte zu sogenannten Gewebekulturen. Ber. dtsch. Bot. Ges.78: 21–29.

    Google Scholar 

  • Meyer, V.G. 1966. Flower abnormalities. Bot. Rev.32: 165–216.

    Google Scholar 

  • Murashige, T. andR. Nakano. 1965. Morphogenetic behaviour of tobacco tissue cultures and implication of plant senescence. Amer. J. Bot.52: 819–827.

    Article  Google Scholar 

  • — and —. 1966. Tissue culture as a potential tool in obtaining polyploid plants. J. Heredity57: 114–118.

    Google Scholar 

  • — and —. 1967. Chromosome complement as a determinant of the morphogenetic potential of tobacco cells. Amer. J. Bot.54: 963–970.

    Article  Google Scholar 

  • — and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant.15: 473–497.

    Article  CAS  Google Scholar 

  • Nishi, T. andS. Mitsuoka. 1969. Occurrence of various ploidy plants from anther and ovary culture of rice. Jap. J. Genetics44: 341–346.

    Google Scholar 

  • Nishiyama, I. andT. Taira. 1966. The effects of kinetin and indoleacetic acid on callus growth and organ formation in two species ofNicotiana. Jap. J. Genetics41: 357–365.

    Google Scholar 

  • Sacristan, M.D. 1971. Karyotypic change in callus cultures from haploid and diploid plant ofCrepis capillaris (L) Wallr. Chromosoma (Berl.)33: 273–283.

    Google Scholar 

  • —. 1969. The cytological analysis of plants regenerated from tumorous and other callus cultures of tobacco. Mol. Gen. Genetics105: 317–333.

    Article  CAS  Google Scholar 

  • Shimada, T. andM. Tabata. 1967. Chromosome numbers in cultured pith tissue of tobacco. Jap. J. Genetics42: 195–201.

    Google Scholar 

  • Straus, J. 1961. Spontaneous changes in corn endosperm tissue cultures. Science128: 537–538.

    Google Scholar 

  • Syono, K. 1965a. Physiological and biochemical changes during successive culturing of plant callus. Plant and Cell Physiol.6: 371–392

    CAS  Google Scholar 

  • — 1965b. Changes in organ forming capacity of carrot root calluses during subculturing. Plant and Cell Physiol.6: 403–419.

    CAS  Google Scholar 

  • Tabata, M., H. Yamamoto andN. Hiraoka. 1968. Chromosome constitution and nicotine formation of mature plants derived from cultured pith of tobacco. Jap. J. Genetics43: 319–322.

    Google Scholar 

  • Takebe, I., G. Labib andG. Melchers. 1971. Regeneration of whole plants from isolated mesophyll protoplasts of tobacco. Naturwiss.58: 318–320.

    Article  Google Scholar 

  • Torrey, J.G. 1967. Morphogenesis in relation to chromosome constitution in long-term plant tissue cultures. Physiol. Plant.20: 265–275.

    Article  Google Scholar 

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Part XVIII in the series “studies on plant tissue cultures”; for Part XVII, see Chem. Pharm. Bull., in press (1972).

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Syōno, K., Furuya, T. Abnormal flower formation of tobacco plants regenerated from callus cultures. Bot Mag Tokyo 85, 273–284 (1972). https://doi.org/10.1007/BF02490173

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  • DOI: https://doi.org/10.1007/BF02490173

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