Abstract
The plantlets regenerated from tobacco calluses, subcultured for prolonged periods, were weak and generally could not be cultivated into mature flowering specimens. However, some of them flowered and various kinds of abnormal flowers as well as leaves were observed in all the tobacco plants bearing flowers. They were sterile at had abnormal immature pollen grains. Some of them occasionally germinated in the anther. Abnormalities were also found in the nucleus of immmature pollen grains. Such drastic floral abnormalities were not found in tobacco plants derived from seeds and callus cultures subcultured for a relatively short period under the same conditions of cultivation.
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Abbreviations
- IAA:
-
3-indolylycetic acid
References
Audus, L.J. 1963. Plant Growth Substances. Leonard Hill Ltd., London.
Gautheret, R.J. 1955. Sur la variabilité des propriétés physiologiques des cultures de tissues végétaux. Ann. Biol.31: 345–371.
Ishihara, A. 1965. On the growth and organ formation of callus tissue in vitro originated from carrot roots (in Japanese). Proc. Crop Sci. Soc. Japan16: 225–233.
Melchers, G. 1965. Einige genetisch Gesichtspunkte zu sogenannten Gewebekulturen. Ber. dtsch. Bot. Ges.78: 21–29.
Meyer, V.G. 1966. Flower abnormalities. Bot. Rev.32: 165–216.
Murashige, T. andR. Nakano. 1965. Morphogenetic behaviour of tobacco tissue cultures and implication of plant senescence. Amer. J. Bot.52: 819–827.
— and —. 1966. Tissue culture as a potential tool in obtaining polyploid plants. J. Heredity57: 114–118.
— and —. 1967. Chromosome complement as a determinant of the morphogenetic potential of tobacco cells. Amer. J. Bot.54: 963–970.
— and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant.15: 473–497.
Nishi, T. andS. Mitsuoka. 1969. Occurrence of various ploidy plants from anther and ovary culture of rice. Jap. J. Genetics44: 341–346.
Nishiyama, I. andT. Taira. 1966. The effects of kinetin and indoleacetic acid on callus growth and organ formation in two species ofNicotiana. Jap. J. Genetics41: 357–365.
Sacristan, M.D. 1971. Karyotypic change in callus cultures from haploid and diploid plant ofCrepis capillaris (L) Wallr. Chromosoma (Berl.)33: 273–283.
—. 1969. The cytological analysis of plants regenerated from tumorous and other callus cultures of tobacco. Mol. Gen. Genetics105: 317–333.
Shimada, T. andM. Tabata. 1967. Chromosome numbers in cultured pith tissue of tobacco. Jap. J. Genetics42: 195–201.
Straus, J. 1961. Spontaneous changes in corn endosperm tissue cultures. Science128: 537–538.
Syono, K. 1965a. Physiological and biochemical changes during successive culturing of plant callus. Plant and Cell Physiol.6: 371–392
— 1965b. Changes in organ forming capacity of carrot root calluses during subculturing. Plant and Cell Physiol.6: 403–419.
Tabata, M., H. Yamamoto andN. Hiraoka. 1968. Chromosome constitution and nicotine formation of mature plants derived from cultured pith of tobacco. Jap. J. Genetics43: 319–322.
Takebe, I., G. Labib andG. Melchers. 1971. Regeneration of whole plants from isolated mesophyll protoplasts of tobacco. Naturwiss.58: 318–320.
Torrey, J.G. 1967. Morphogenesis in relation to chromosome constitution in long-term plant tissue cultures. Physiol. Plant.20: 265–275.
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Part XVIII in the series “studies on plant tissue cultures”; for Part XVII, see Chem. Pharm. Bull., in press (1972).
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Syōno, K., Furuya, T. Abnormal flower formation of tobacco plants regenerated from callus cultures. Bot Mag Tokyo 85, 273–284 (1972). https://doi.org/10.1007/BF02490173
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DOI: https://doi.org/10.1007/BF02490173