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A model for antiHelicobacter pylori chemotherapy using an experimental murine infection withHelicobacter felis

  • Original Articles
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Journal of Infection and Chemotherapy

Abstract

Colonization ofHelicobacter felis was observed to continue for at least 24 weeks in a BALB/c mouse stomach after oral inoculation of the bacteria. Bacteria were observed on the surface of the gastric mucosa and in the gastric pits as is typically seen withHelicobacter pylori infection in the human stomach.H. felis-colonized mice were treated for 5 consecutive days with various chemotherapeutic regimens used againstH. pylori for humans. The status ofH. felis infection was then assessed by the urease test 2 weeks after the final treatment. The clearance rates were 100% for a triple combination of amoxicillin (15 mg/kg), metronidazole (10 mg/kg) and bismuth subnitrate (5 mg/kg), 71% for amoxicillin alone (15 mg/kg), 80% for metronidazole alone (15 mg/kg), and 0% for bismuth subnitrate (60 mg/kg) or omeprazole (60 mg/kg) alone. Bacterial recurrence occurred more often in mice treated with amoxicillin alone than with the triple regimen during the 24-week posttreatment period. These results were very similar to the efficacy of each regimen obtained in clinical trials. Thus, this mouse model usingH. felis may be useful for evaluating drugs or regimens for the treatment of humanH. pylori infections.

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Authors and Affiliations

  1. Shionogi Research Laboratories, Shionogi and Co. Ltd., 3-1-1 Futaba-cho, 561-0825, Toyonaka, Osaka, Japan

    Yoshinao Kobayashi, Masayoshi Doi, Hiroshi Nagata, Kazunori Sasaki, Toshiyuki Maruyama & Kazuhisa Murakami

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  1. Yoshinao Kobayashi
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  2. Masayoshi Doi
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  3. Hiroshi Nagata
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  4. Kazunori Sasaki
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  5. Toshiyuki Maruyama
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  6. Kazuhisa Murakami
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Kobayashi, Y., Doi, M., Nagata, H. et al. A model for antiHelicobacter pylori chemotherapy using an experimental murine infection withHelicobacter felis . J Infect Chemother 4, 195–200 (1998). https://doi.org/10.1007/BF02490166

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  • DOI: https://doi.org/10.1007/BF02490166

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