Abstract
Background
It is now possible for leukocyte-depletion filters to be used for removing malignant cells from blood during autotransfusion in patients with malignant tumors. With the objective of ascertaining its clinical application in gynecologic surgery for malignant tumors, we have performed a basic investigation of the malignant cell depletion performance of the BPF4-BJ, a new, fourth-generation leukocyte-depletion filter.
Methods
We used 9 cell lines derived from malignant tumors of gynecologic origin. The cells were suspended in phosphate buffered saline without magnesium and calcium (PBS[-]) and passed through a BPF4-BJ leukocyte-depletion filter. The filter's depletion performance was determined by the decrease in cultured cells. The number of cells was determined by using an automatic cell counter and visual inspection, and by morphologic examination using scanning electron microscopy. In addition, OMC-9 cells were suspended in human blood, and depletion performance was measured in a similar manner.
Results
All cell lines that were suspended in PBS(-) were adsorbed on the filter fibers, and the number of cells passing through the filter was below the detection limit. No cultured cells were observed after cytodiagnosis of the filtrate. Similarly all of the OMC-9 cells that were suspended in human blood were adsorbed on the filter.
Conclusion
Leukocyte-depletion filters can be used to remove cells that are cultured from malignant tumors. Such filters may have a clinical application, not only in predeposit techniques, but also in the intraoperative autologous transfusion of salvaged blood from patients with malignant tumors of gynecologic origin.
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References
Miller GV, Ramsden CW, Primrose JN. Autologous transfusion: an alternative to transfusion with banked blood during surgery for cancer. Br J Surg 1991;78:713–715.
Wiesel M, Gudemann C, Staehler G, Bierhaus A, Martin E, Hoever KH. Separation von urologischen Tumorzellen aus Cell-Saver-Blut durch Einsatz eines Membranfilters. Urologe A 1992;31:182–185.
Osawa T, Nakamura S, Imai T. Intraoperative blood recovery in transurethral resection of prostate (TURP). Jap J Urol (in Japanese, abstract in English) 1992;83:1276–1283.
Yoshida M, Kawano K, Sugihara K, Yamashita S. Experimental study on the removal of cancer cells through a new leucocyte depletion filter. J Jpn Stomatol Soc (in Japanese, abstract in English) 1995;44:408–411.
Kojima O, Park K, Kim CJ, Tomoyoshi T. Intraoperative autotransfusion during surgery for cancer—effects of leukocyte removal filters for separation of tumor cells. J Jap Soc Autologous Blood Transfusion (in Japanese) 1995;8:185–188.
Yamada T, Ueda M, Maeda T, Okamoto Y, Miyawaki Y, Otsuki Y, Ueki M, Sugimoto M. Establishment and characterization of CA 125 producing cell line (OMC-2) originating from a human endometrial adenocarcinoma. Asia-Oceania J Obstet Gynaecol 1989;15:403–416.
Ueda M, Ueki M, Yamada T, Okamoto Y, Maeda T, Sugimoto O, Otsuki Y. Scatchard analysis of EGF receptor and effects of EGF on growth and TA-4 production of newly established uterine cervical cancer cell line (OMC-1). Human Cell 1989;2:401–410.
Nozawa S, Udagawa Y, Ohta H, Kurihara S, Fishman WH. Newly established uterine cervical cancer cell line (SKG-III) with Regan isoenzyme, human chorionic gonadotropin β-subunit, and pregnancy-specific β1-glycoprotein phenotypes. Cancer Res 1983;43:1748–1760.
Yamada T, Ueda M, Maeda T, Miyawaki Y, Misaki O, Ueki M, Sugimoto O. Establishment and characterization of a cell line (OMC-4) originating from a human adenocarcinoma of the uterine cervix. Acta Obst Gynaec Jpn 1987;39:859–860.
Ueda M, Okamoto Y, Yamada T, Seiki Y, Misaki O, Tsurunaga T, Iwai E, Fukuda Y, Maeda Y, Ueki M, Sugimoto O, Otsuki Y. Endometrial stromal sarcoma and its cell culture. Adv Obstet Gynecol (in Japanese, abstract in English) 1990;42:683–691.
Kanda T, Yamada T, Okamoto Y, Ikeda A, Okamoto Y. Establishment and characterization of two cell lines originating from a human endometrial stromal sarcoma of the uterus. Acta Obst Gynaec Jpn (in Japanese) 1994;46:S-370.
Ishiwata I, Nozawa S, Nagai S, Kurihara S, Mikata A, Okumura H. Establishment of a human leiomyosarcoma cell line. Cancer Res 1977;37:658–664.
Dale RF, Kipling RM, Smith MF, Collier DSJ, Smith PJ. Separation of malignant cells during autotransfusion. Br J Surg 1988;75:581.
Fujimoto J, Okamoto E, Yamanaka N, Fujiwara S, Oriyama T, Kato T, Furukawa K, Toyosaka A. Autologous blood transfusion in hepatectomy—advantages & pitfalls. J Jap Soc Autologous Blood Transfusion (in Japanese) 1989;2:62–65.
Fukui A, Kimura K, Takaori M. Study on removal of malignant tumor cells in salvaging autologous blood transfusion—with special reference to the cytocidal effect of MMC on malignant tumors. Jap J Transfusion Med (in Japanese, abstract in English) 1992;38:508–514.
Lane TA, Anderson KC, Goodnough LT, Kurtz S, Moroff G, Pisciotto PT, Sayers M, Silberstein LE. Leukocyte reduction in blood component therapy. Ann Intern Med 1992;117:151–162.
Yamamura H, Miyahara M, Satoh H. The effects of filtration procedures and blood storage period on the efficiencies of polyester filters for preparning leukocyte reduced red cells. Jap J Transfusion Med (in Japanese, abstract in English) 1994;40:655–660.
Yoshino Y, Takama T, Kawashima M, Tsubokura M, Takenaka M, Amaki I. Preparation of leukocyte-poor red cells for transfusion by a newly-developed BPF4 filter. Blood Programme (in Japanese) 1992;15:553–560.
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Yamada, T., Terai, Y., Yamashita, Y. et al. Removal of malignant cells through a leukocyte-depletion filter for autologous blood transfusion. Int J Clin Oncol 2, 143–146 (1997). https://doi.org/10.1007/BF02490124
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DOI: https://doi.org/10.1007/BF02490124