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Epidemiologic typing of methicillin-resistantStaphylococcus aureus by macrorestriction analysis of genomic DNA using two different restriction enzymes

  • Original Articles
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Journal of Infection and Chemotherapy

Abstract

Ninety-seven methicillin-resistantStaphylococcus aureus (MRSA) isolates from patients, staff and the environment in a teaching hospital in Japan were typed by pulsed-field gel electrophoresis (PFGE). Two different macrorestriction enzymes (SmaI andSstII) were used to digest the DNA. In this study, after identifying the dominant pattern, the isolates were classified into 4 categories including indistinguishable, closely related, possibly related, and unrelated. While closely related isolates were identified as a subtype, possibly related isolates showing differences of 4 to 6 fragments were difficult to identify either as a subtype or different type. Although there were similar trends in isolate identification by restriction patterns ofSmaI andSstII, some isolates showed a discrepancy. There were 9 isolates categorized as possibly related by eitherSmaI orSstII restriction pattern, and while 2 of the 9 displayed possibly related patterns by both enzymes, 7 showed discrepancies. Among the latter 7 isolates, 1 displayed an indistinguishable pattern by the other macrorestriction enzyme, 5 showed closely related patterns, and 1 showed an unrelated pattern. These discrepancies may help delineate a more precise differentiation of the isolates. Indices of the discriminatory ability of PFGE typing bySmaI andSstII were 0.768 and 0.742. The combination of these 2 enzymes led to an increase in discrimination (D=0.8045). The use of PFGE typing bySmaI andSstII in parallel may enhance discrimination.

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Authors and Affiliations

  1. Department of Laboratory Medicine, National Defense Medical College, Saitama, Japan

    Katsu Saionji

  2. Department of Clinical Laboratory, Juntendo Hospital, Tokyo, Japan

    Aayako Nakamura

  3. Department of Clinical Pathology, Juntendo University School of Medicine, 2-1-1 Bunkyo-ku, 113-8421, Tokyo, Japan

    Yoko Tabe & Jun Igari

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  1. Yoko Tabe
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  2. Katsu Saionji
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  3. Aayako Nakamura
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  4. Jun Igari
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Tabe, Y., Saionji, K., Nakamura, A. et al. Epidemiologic typing of methicillin-resistantStaphylococcus aureus by macrorestriction analysis of genomic DNA using two different restriction enzymes. J Infect Chemother 4, 56–63 (1998). https://doi.org/10.1007/BF02489962

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  • DOI: https://doi.org/10.1007/BF02489962

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