Abstract
On the basis of an already established suspension system of organ culture for mouse palate explants, we developed a new culture system, which has several advantages over the previous methods. We used a 48-well culture plate in which the explants can be cultured indivisually, and only 300 μ 1 of medium is needed for each well. In order to optimize the culture results, we systematically studied the influence of main “culture conditions” such as tilt degree of the culturing palate, rotation speed, and addition of ascorbic acid to the medium. This system allows culturing of palates from day 13.5 of gestation to day 16.5 under serum-free conditions using a chemically defined medium, which resulted in 78% of the palates growing fused. Utilizing this culture system, the direct effect of X-irradiation on palataogesis was analyzed. A 4 Gy dose of X-irradiation was administrated at the beginning of culture period. The incidence of palatal fusion was not significantly different from that of the non-irradiated group.
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Greene, R.M. & Pratt, R.M.: Developmental aspects of secondary palate formation.J Embryol Exp Morphol. 36: 225–45, 1976.
Ferguson, M.W.: Palate development.Development 103 (Suppl.): 41–60, 1988.
Reeve, W.L., Porter, K. & Letkowitz, W.: In vitro closure of the rat palate.J Dent Res. 45: 1375–1380, 1966.
Vargas, V.I.: Palatal fusion in vitro in the mouse.Archs Oral Biol. 12: 1283–1288, 1967.
Lahti, A., Antila, E. & Saxen, L.: The effect of hydrocortisone on the closure of the palatal shelves in two inbred strains of micein vivo and in vitro.Teratology 6: 37–42, 1972.
Myers, G.S., Petrakis, N.L. & Lee, M.: Cultivation of the embryonic rat palate in defined and semi-defined media.Archs Oral Biol. 12: 565–567, 1967.
Shiota, K., Kosazuma, T., Klug, S. & Neubert, D.: Development of the fetal mouse palate in suspension organ culture.Acta Anat (Basel) 137: 59–64, 1990.
Al-Obaidi, N., Kastner, U., Merker, H. & Klug, S.: Development of a suspension organ culture of the fetal rat palate.Arch Toxicol. 69: 472–479, 1995.
Russell, L.B. & Russell, W.L.: An analysis of the changing radiation response of the developing mouse embryo.J Cell Physiol. 43: 1030–1149, 1954.
Saad, A.Y., Abdelazim, A.A., el-Khashab, M.M. & Mansour, M.A.: Induction of cleft palate by gamma-irradiation of prenatal CD-1 mice.Cleft Palate Craniofac J. 31: 351–5, 1994.
Collins, D., Leung, R., Gartner, L.P. & Hiatt, J.L.: Induction of cleft palate by X-irradiation of prenatal mice.J Baltimore Coll Dent Surg. 31: 54–60, 1976.
Theiler, K.:The house mouse: atlas of embryonic development, pp. 1–178, Spring-Berlag, New York, 1989.
Miyake, T., Cameron, A.M. & Hall, B.K.: Detailed staging of inbred C57BL/6 mice betwween Theiler's [1972] stages 18 and 21 (11–13 days of gestation) based on craniofacial development.J Craniofac Gene Dev Biol. 16: 1–31, 1996.
Brunet, C.L., Sharpe, P.M. & Ferguson, M.W.: Inhibition of TGF-beta 3 (but not TGF-beta 1 or TGF-beta 2) activity prevents normal mouse embryonic palate fusion.Int J Dev Biol. 39: 345–55, 1995.
Hausman, E.: Cofactor requirements for the enzymatic hydroxylation of lysine in a polypeptide precursor of collagen.Biochem Biophys Acta. 133: 591–593, 1967.
Blanck, T.J.J. & Peterkofsky, B.: The stimulation of collagen secretion by ascorbate as a result of increased proline hydroxylation.Arch. Biochem Biophys. 171: 259–267, 1975.
Kandasamy, S.B., Thiagarajan, A.B. & Harris, A.H.: Possible involvement of prostaglandins in increases in rat plasma adrenocorticotropic hormone and corticosterone levels induced by radiation and interleukin-1 alpha, alone or combined.Fundam Appl Toxicol. 25: 196–200, 1995.
Lehy, T., Dessirier, V., Attoub, S., Bado, A., Griffiths, N.M. & Linard, C.: Exposure to ionizing radiation modifies circulating gastrin levels and gastrointestinal endocrine cell densities in the rat.Int J Radiat Biol. 73: 331–40, 1998.
Esposito, V., Linard, C., Maubert, C., Aigueperse, J. & Gourmelon, P.: Modulation of gut substance P after whole-body irradiation. A new pathological feature.Digest Dis Sci. 41: 2070–2077, 1996.
Nomura, T., Hata, S. & Kusafuka, T.: Suppression of developmental anomalies by maternal macrophages in mice.J Exp Med. 172: 1325–1330, 1990.
Abbott, B.D.: Review of the interaction between TCDD and glucocorticoids in embryonic palate.Toxicology 105: 365–373, 1995.
Abbott, B.D., Harris, M.W. & Birnbaum, L.S.: Etiology of retinoic acid-induced cleft palate varies with the embryonic stage.Teratology 40: 533–553, 1989.
Pratt, R.M., Kim, C.S. & Grove, R.I.: Role of glucocorticoids and epidermal growth factors in normal and abnormal palatal development.Curr Top Dev Biol. 19: 81–101, 1984.
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Hiranuma, H., Jikko, A., Maeda, T. et al. Development of a new mouse palate organ culture system and effect of X-irradiation on palatogenesis. Oral Radiol. 15, 117–123 (1999). https://doi.org/10.1007/BF02489650
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DOI: https://doi.org/10.1007/BF02489650