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Specific arrangements of cortical microtubules are correlated with the architecture ofmeristems in shoot apices of angiosperms and gymnosperms

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Abstract

Arrangements of corticalmicrotubules (MTs) as seen in median longitudinal cryosections of shoot apices of several angiosperms and gymnosperms were studied by indirect immunofluorescence microscopy.Bryophyllum, Clethra, Helianthus, Houttuynia, Vinca (angiosperms), andPinus, Cedrus, Cedrus andGinkgo (gymnosperms) were examined. In all angiosperm apices collected during the growing season, MTs were mainly arranged anticlinally in the tunica, randomly in the corpus, and transversely in the rib meristem. This pattern of arrangements of MTs was further confirmed by electron microscopy inBryophyllum apices. In the apices of winter shoots MTs in the rib meristem were arranged randomly, indicating a seasonal change with respect to their arrangment. In all examined gymnosperm apices, populations of superficial cells showed both random and anticlinal arrangements of MTs, in contrast to those of angiosperm apices that consistently show anticlinally arranged MTs. In the shoot apices of both angiosperms and gymnosperms, cortical MTs were arranged perpendicularly to the directions of cell expansion. The significance of MTs in the maintrnance of the different architectures of shoot apices in angiosperms and gymnosperms is discussed.

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Abbreviations

FITC:

fluorescein isothiocyanate

MT(s):

microtubule(s)

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Author notes

  1. Shuichi Sakaguchi

    Present address: Division of Cell Proliferation, Department of Cell Biology, National Institute for Basic Biology, Okazaki National Research Institutes, 444, Okazaki

  2. Taizo Hogetsu

    Present address: Laboratory of Forest Botany, Faculty of Agriculture, University of Tokyo, Bunkyo-ku, 113, Tokyo

Authors and Affiliations

  1. Department of Biology, College of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, 153, Tokyo

    Shuichi Sakaguchi & Noboru Hara

  2. Department of Pure and Applied Sciences, College of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, 153, Tokyo

    Taizo Hogetsu

Authors
  1. Shuichi Sakaguchi
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  2. Taizo Hogetsu
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  3. Noboru Hara
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Sakaguchi, S., Hogetsu, T. & Hara, N. Specific arrangements of cortical microtubules are correlated with the architecture ofmeristems in shoot apices of angiosperms and gymnosperms. Bot Mag Tokyo 103, 143–163 (1990). https://doi.org/10.1007/BF02489622

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  • DOI: https://doi.org/10.1007/BF02489622

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