Abstract
Light-induced absorbance changes were studied for brown algae with 23 species and a pronounced absorbance change around 563 nm was found in all algae examined.
3-(3,4-dichlorophenyl)-1,1-dimethylurea and gramicidin J suppressed the initial rate and the magnitude of the absorbance change. Carbonylcyanidem-chlorophenylhydrazone did not affect the initial rate but decreased the maximum level of the change.
All thalli and the chloroplasts tested had an absorption band at around 540 nm due to fucoxanthin which accounted for about 70–90% of the total carotenoids in brown algae. It is proposed that the 563 nm-change is caused by the red shift of fucoxanthin responding to the light-induced change in the membrane potential of the thylakoid system.
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Abbreviations
- Cl-CCP:
-
carbonylcyanidem-chlorophenylhydrazone
- DCMU:
-
3-(3,4-dichlorophenyl)-1,1-dimethylurea
- MES:
-
2-(N-morpholino)ethane sulfonic acid
References
Anderson, J.M. andJ. Barrett. 1979. Chlorophyll-protein complexes of brown algae: P700 reaction center and light-harvesting complexes.In: Chlorophyll Organization and Energy Transfer in Photosynthesis. Ciba Foundation Symp.61: 81–104. Excerpta Medica, Amsterdam.
Chua, N.-H. andN.I. Bishop. 1969. The 520 nm light-induced absorbance change ofChlamydomonas reinhardii VIII The 520 nm light-induced absorbance change in the wild-type and mutant strains. Plant Physiol.44: 1–6.
Davies, B.H. 1965. Analysis of carotenoid pigments.In: T.W. Goodwin, ed., Chemistry and Biochemistry of Plant Pigments p. 489–532. Academic Press, London.
Fork, D.C. andJ. Amesz. 1967. Light-induced shift in the absorption spectrum of carotenoids in red and brown algae. Photochem. Photobiol.6: 913–918.
—. 1976. A comparison of light-induced shifts in carotenoid absorption in representations of different algal groups. Carnegie Inst. Washington Year Book74: 776–779.
Goedheer, J.C. 1970. On the pigment system of brown algae. Photosynthetica4: 97–106.
Heytler, P.G. andW.W. Prichard. 1962. A new class of uncoupling agents-carbonylcyanide phenylhydrazones. Biochem. Biophys. Res. Commun.7: 272–275.
Inoué, H., K. Sugahara andM. Nishimura. 1971a. Enery- and electron-transfer reactions in brown algae.In: K. Nisizawa, ed., Proceedings of the Seventh International Seaweed Symp. p. 515–521. Univ. Tokyo Press, Tokyo.
—. 1971b. Light-induced absorbance changes at 475–515 nm and electron flow close to photosystem II in Tris-washed chloroplast fragments. Plant Cell Physiol.12: 457–460.
Jeffrey, S.W. 1968. Quantitative thin-layer chromatography of chlorophylls and carotenoids from marine algae. Biochim. Biophys. Acta162: 271–285.
Junge, W. andH.T. Witt. 1968. On the ion transport system of photosynthesis. Investigation on a molecular level. Z. Naturforsch.23b: 244–254.
Karlish, S.J.D., N. Shavit andM. Avron. 1969. On the mechanism of uncoupling in chloroplasts by ion-permeability inducing agents. Eur. J. Biochem.9: 291–298.
Kunifugi, Y., K. Nakayama andM. Okada. 1977. Distribution of the light-induced 561 nm absorbance change in Chlorophyceae: Carotenoids responsible for the absorbance change.In: S. Miyachiet al., ed., Photosynthetic Organelles. Special Issue Plant Cell Physiol.3: 173–178. Center for Academic Publications, Tokyo.
Larkum, A.W.D. andW.D. Bonner. 1972. Light-induced absorbance changes of P518 in intact chloroplasts. Biochim. Biophys. Acta256: 396–408.
Mann, J.E. andJ. Myers. 1968. Photosynthetic enhancement in the diatomPhaeodactylum tricornutum. Plant Physiol.43: 1991–1995.
Nakayama, K., M. Okada andA. Takamiya. 1974. A new light-induced absorbance change at 561 nm in chloroplasts of green alga,Bryopsis maxima. Plant Cell Physiol.15: 799–805.
Nordhorn, G., M. Weidner andJ. Willenbrink. 1976. Isolation and photosynthetic activities of chloroplasts of the brown algaFucus serratus L. Z. Pflanzenphysiol.80: 153–165.
Reich, R., R. Scheerer, K.-U. Sewe andH.T. Witt. 1976. Effect of electric fields on the absorption spectrum of dye molecules in lipid layers. V. Refined analysis of the field-indicating absorption changes in photosynthetic membranes by comparison with electrochromic measurements in vitro. Biochim. Biophys. Acta449: 285–294.
Rubinstein, D. 1965. Photoinduced absorbance change at 520 nm inChlorella and their relationship to the two pigment system of photosynthesis. Biochim. Biophys. Acta109: 41–44.
Satoh, K. andS. Katoh. 1979. Two electrogenic mechanisms contributing to the 560 nm absorbance changes in intactBryopsis chloroplasts. Biochim. Biophys. Acta545: 454–465.
Strain, H.H. andW.A. Svec. 1966. Extraction, separation, estimation and isolation of the chlorophylls.In: L.P. Vernon, ed., The Chlorophylls p. 21–66. Academic Press, London.
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Nakayama, K., Abe, K. & Okada, M. Light-induced absorbance changes of carotenoids in brown algae. Bot Mag Tokyo 96, 29–36 (1983). https://doi.org/10.1007/BF02489572
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DOI: https://doi.org/10.1007/BF02489572