Skip to main content
SpringerLink
Log in

Application of the Nadi reaction to rice-peroxidase isozyme stain

  • Short Communication
  • Published:
The botanical magazine = Shokubutsu-gaku-zasshi Aims and scope Submit manuscript

Abstract

The Nadi (naphthol and diamine) reaction was applied to rice-peroxidase stains on starch gels, where various amine-phenol combinations were used in the presence of hydrogen peroxide. These combinations brought about specific color products on the gels. Among these,o-dianisidine-2-naphthol gave the largest number of detectable bands of comparatively stable color.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Brewbaker, J.L., M.D. Upadhya, Y. Mäkinen andT. Macdonald. 1968. Isoenzyme polymorphism in flowering plants III. Gel electrophoretic methods and applications. Physiol. Plant.21: 930–940.

    Article  CAS  Google Scholar 

  • Burstone, M.S. 1962. Enzyme Histochemistry and Its application in the Study of Neoplasms. Acad. Press, London.

    Google Scholar 

  • Endo, T.. 1968. Indoleacetate oxidase activity of horseradish and other plant peroxidase isozymes. Plant and Cell Physiol.9: 333–341.

    CAS  Google Scholar 

  • Evans, J.J. andN.A. Alldridge. 1965. The distribution of peroxidases in extreme dwarf and normal tomato (Lycopersicon esculentum). Phytochem.4: 499–503.

    Article  CAS  Google Scholar 

  • Jensen, W.A.. 1962. Botanical Histochemistry-Principles and Practice. Freeman and Co., San Francisco.

    Google Scholar 

  • Kawashima, N. andI. Uritani. 1963. Occurrence of peroxidases in sweet potato infected by the black-rot fungus. Agr. Biol. Chem.27: 409–417.

    CAS  Google Scholar 

  • Lison, L.. 1960. Histochimie et Cytochimie Animales. Principes et Méthodes. Gauthier-Villars and Co., Paris. (Sosikikagaku Oyobi Saibokagaku, translated by T. Imaizumi, 1962, Hakusuisha, Tokyo).

    Google Scholar 

  • Moustafa, E.. 1963. Peroxidase in root nodules of various leguminous plants. Nature199: 1189.

    Article  CAS  Google Scholar 

  • Novacky, A. andR.E. Hampton. 1968. The effect of substrate concentration on the visualization of isoperoxidases in disc electrophoresis. Phytochem.7: 1143–1145.

    Article  CAS  Google Scholar 

  • Staples, R.C. andM.A. Stahmann. 1964. Changes in proteins and several enzymes in susceptible bean leaves after infection by the bean rust fungus. Phytopathol.54: 760–764.

    CAS  Google Scholar 

  • Van Fleet, D.S.. 1952. Histochemical localization of enzymes in vascular plants. Bot. Rev.18: 354–398.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Institute of Genetics, 411, Misima

    Toru Endo

Authors
  1. Toru Endo
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Contribution No. 899 from the National Institute of Genetics, Misima.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Endo, T. Application of the Nadi reaction to rice-peroxidase isozyme stain. Bot Mag Tokyo 85, 147–151 (1972). https://doi.org/10.1007/BF02489511

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02489511

Keywords

Search

Navigation