Abstract
Streptomycin sulphate (2 mg/ml) did not affect the formation of proplastids or the elaboration of prolamellar bodies. The plastids of the streptomycin (SM)-treated cotyledons contained both crystalline prolamellar bodies and ribosomes, and were undistinguishable from the plastids of the water-grown cotyledon. However, plastids from dark-grown SM-treated cotyledons were no longer able to differentiate to more advanced stages of development, even after exposure to light. The plastids of light and dark-grown SM-treated cotyledons often contained prolamellar bodies and abnormal giant grana. Variegation developed in the cotyledons germinated in Hoagland's solution plus SM. The plastids in pale green tissue contained stroma-lamellae and one or two giant grana, whereas in those of pale yellow tissue, many osmiophilic globules, large vacuoles and crystal bodies were observed. It is suggested that the formation of prolamellar bodies may depend on cytoplasmic protein synthesis whereas functional stroma- and grana-lamellae may depend on protein synthesis within the plastids. The inhibitory effects of SM on protein synthesis were used as a tool to test this hypothesis.
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References
Ben-Shaul, Y. andI. Ophir. 1970. Effects of streptomycin on plastids in dividingEuglena. Planta91: 195–203.
Brody, I. 1958. The keratinization of epidermal cells of normal guinea pig skin as revealed by electron microscopy. J. Ultrastruct. Res.2: 482–511.
Clark, M.F., R.E.F. Mathews andR.K. Ralph. 1964. Ribosomes and polysomes inBrassica pekinensis. Biochim. Biophys. Acta91: 289–304.
— 1964. Polysomes from chloroplasts. Biochim. Biophys. Acta91: 671–674.
Cox, C.E., J.R. White andJ.G., Falks. 1964. Streptomycin action and the ribosome. Proc. Nat. Acad. Sci. U.S.A.51: 703–709.
Davies, J., W. Gilbert andL. Gorini. 1964. Streptomycin suppression and the code. Proc. Nat. Acad. Sci. U.S.A.51: 883–890.
—,P. Anderson andB.D. Davis. 1965. Inhibition of protein synthesis by streptomycin. Science149: 1096–1097.
De Deken-Grenson, M. 1955. Action de la streptomycin sur la formation des chloroplasts. Biochim. Biophys. Acta17: 35–47.
Döbel, P. 1963. Untersuchung der Wirkung von Streptomycin-, Chloramphenicol- und 2-Thiouracil-Behandlung auf die Plastidenentwicklung vonLycopersicon esculentum Miller. Biol. Zbl.82: 275–295.
Ellis, R.J. 1969. Chloroplast ribosomes: Stereospecificity of inhibition by chloramphenicol. Science163: 477–478.
Heslop-Harrison, J. 1963. Structure and morphogenesis of lamellar systems. I. Membrane structure and lamellar architecture. Planta60: 243–260.
Ikeda, T. 1968. Analytical studies on the structure of prolamellar body, Bot. Mag. Tokyo81: 517–527.
Khudairi, A.K. 1961.Xanthium leaf pigments and their inhibition by streptomycin. Biochim. Biophys. Acta46: 344–354.
Kirk, J.T.O. 1962a. Effect of streptomycin on greening and biosynthesis inEuglena gracilis. Biochim. Biophys. Acta56: 139–151.
— 1962b. Effect of streptomycin on14C leucine incorporation inEuglena gracilis. Biochim. Biophys. Acta59: 476–479.
— andB.E. Juniper. 1963. The effect of streptomycin on the mitochondria and plastids of barley. Exptl. Cell Res.30: 621–623.
Kirk, J.T.O. and R.A.E. Tilney-Bassett. 1967. Chapter XV, 524. The plastids. W. H. Freeman and Co.
Luzzatto, L., D.D. Apiron andD. Schlessinger. 1968. Mechanism of action of streptomycin inE. coli: Interruption of the ribosome cycle at the initiation of protein synthesis. Proc. Nat. Acad. Sci. U.S.A.60: 873–880.
Lyttleton, J.W. 1962. Isolation of ribosomes from spinach chloroplats. Exptl. Cell Res.26: 312–317.
Mangiarotti, G. andD. Schlessinger. 1967. Polysome metabolism inEscherichia coli. II. Formation and lifetime of messenger-RNA molecules, ribosomal subunit couples and polyribosomes. J. Mol. Biol.29: 395–418.
Marcus, A. andJ. Feeley. 1964. Activation of protein synthesis in the inhibition phase of seed germination. Proc. Nat. Acad. Sci. U.S.A.51: 1075–1079.
Margulies, M.M. 1966. Effect of chloramphenicol on formation of chloroplast structure and protein during greening of etiolated leaves ofPhaselus vulgaris. Plant Physiol.41: 992–1003.
Mühlethaler, K., H. Moor andJ.W. Szarkowski. 1965. The ultrastructure of the chloroplast lamellae. Planta67: 305–323.
Reynolds, E.S. 1963. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol.,17: 208–212.
Rosen, W.G. 1957. A possible mechanism for inhibition of plant growth by streptomycin. Plant Physiol.32: viii.
—. 1961. Effect of streptomycin on chlorophyll accumulation inEuglena gracilis. J. Protozool.8: 90–96.
—. 1961. Some observations on the fine structure of a thermophilic, acidophilic alga. J. Biophys. Biochem. Cytol.9: 910–914.
Schötz, F. 1965. Zur Frage der Vermehrung der Thylakoidschiten in den Chloroplasten. Planta64: 376–380.
Siegesmund, K.A., E.G. Rosen andS.R. Gawlik. 1962. Effects of darkness and of streptomycin on fine structure ofEuglena gracilis. Amer. J. Bot.49: 137–145.
Spencer, D. andS.G. Wildman, 1964. The incorporation of amino acids into protein by cell-free extracts from tobacco leaves. Biochemistry3: 954–959.
Srivastava, L.M., M. Vesk andA.P. Singh. 1971. Effect of chloramphenicol on membrane transformation in plastids. Can. J. Bot.49: 587–593.
Stutz, E. andH. Noll. 1967. Characterization of cytoplasmic and chloroplast polysomes in plants: Evidence for three classes of ribosomal RNA in mature. Proc Nat. Acad. Sci. U.S.A.57: 774–781.
Tôyama, S. 1971. Electron microscope studies on the morphogenesis of plastids VI. Plastid development and fine structure in variegated leaves of tomato. Bot. Mag. Tokyo85: 1–10.
Wehrmeyer, W. 1965. Zur Kristallgitterstruktur der sogenannten Prolamellarkörper in Proplastiden etiolierten Bohnen. I. II. III. Z. Naturforsch.20b: 1270–1296.
Weier, T.E., C.R. Stocking, C.E. Bracker andE.B. Risley. 1965. The structural relationships of the internal membrane system ofin situ and isolated chloroplasts ofHordeum vulgare. Amer. J. Bot.52: 339–352.
— andD.L. Brown. 1970. Formation of prolamellar body in 8-day, dark-grown seedling. Amer. J. Bot.57: 267–275.
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This work was carried out in the Department of Botany, University of California, Davis, by Grant-GB-11906 from National Science Foundation of U.S.A.
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Tôyama, S. Electron microscope studies on the morphogenesis of plastids. Bot Mag Tokyo 85, 89–103 (1972). https://doi.org/10.1007/BF02489506
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DOI: https://doi.org/10.1007/BF02489506