Abstract
Possible roles of cell wall and cytoplasmic peptides in the tolerance of cells to Cu2+ and Cd2+ ions were studied in suspension-cultured cells of tomato (Lycopersicon esculentum L. cv. Palace). Cu2+ and Cd2+ ions inhibited growth of wild type cells at concentrations more than 100 and 200 μM, respectively. Tomato cells readily developed tolerance to Cd2+ ions up to 1 mM but not to Cu2+ ions, after repeated subculturings in the presence of the respective ions. Such a metal-specific adaptation of cells was not due to the difference in the total uptakes between Cd2+ and Cu2+ ions by cells. Wild-type cells accumulated Cd2+ preferentially into the cytoplasmic peptide fraction and Cu2+ into the cell-wall fraction, when grown under the subtoxic metal conditions. Under excess metal conditions, Cd-tolerant cells produced greater amounts of Cd-binding peptides in the cytoplasm and retained lesser amounts of Cd2+ ions in the cell wall than did wild-type cells. In contrast, tomato cells grown in the presence of Cu2+ ions synthesized no detectable amounts of Cu-binding peptides in the cytoplasm and retained most of the Cu2+ in the cell-wall fraction, irrespective of cell lines.
These results suggested that the cytoplasmic peptides rather than cell wall properties have a primary role in the response of tomato cells to excess metal environments.
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Abbreviations
- GPC:
-
gel-permeation chromatography
- HPLC:
-
high performance liquid chromatography
- MT:
-
metallothionein
- PC:
-
phytochelatin
- PCMB:
-
p-chloromercuribenzoate
- TFA:
-
trifluoroacetic acid
- TTC:
-
2,3,5-triphenyltetrazolium chloride
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Inouhe, M., Mitsumune, M., Tohoyama, H. et al. Contributions of cell wall and metal-binding peptide to Cd- and Cu-tolerances in suspension-cultured cells of tomato. Bot Mag Tokyo 104, 217–229 (1991). https://doi.org/10.1007/BF02489454
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DOI: https://doi.org/10.1007/BF02489454