Abstract
Cycloheximide retarded the loss of chlorophyll from detached komatsuna (Brassica campestris cv. Komatsuna) leaves during incubation in the dark but promoted its loss in light. Cycloheximide-induced chlorophyll bleaching in light was prevented by some active oxygen scavengers. Chloroplast envelopes of cycloheximide-treated leaves incubated in both the dark and light were destroyed within 48 h. The grana of cycloheximide-treated leaves incubated in light were dilated.
Abbreviations
- Tiron:
-
1,2-dihydroxybenzen-3,5-disulfonic acid
- DMTU:
-
1,3-dimethylthiourea
References
Ellis, R.J. 1969. Does cycloheximide inhibit protein synthesis specifically in plant tissues? Nature222: 791–792.
—, andI.R. Macdonald 1970. Specificity of cycloheximide in higher plant systems. Plant Physiol.46: 227–232.
Kaur-Sawhney, R. andA.W. Galston 1979. Interaction of polyamines and light on biochemical processes involved in leaf senescence. Plant Cell Environment2: 189–196.
Kotaka, S. andA.P. Krueger 1969. Some observation on the bleaching effect of ethylen-diaminetetraacetic acid on green barley leaves. Plant Physiol.44: 809–815.
Martin, C. andK.V. Thimann 1972. The role of protein synthesis in the senescence of leaves I. The formation of protenase. Plant Physiol.49: 64–71.
Matsunaka, S. 1988. Paraquat, An active-oxygen producing herbicide. Protein, Nucleic acid and Enzyme33: 2790–2794.
Pjon, C.J. 1981. Effects of cycloheximide and light on leaf senescence in maize and hydrangea. Plant Cell Physiol.22: 847–854.
— 1982. Effects of metal chelators on leaf senescence in maize and hydrangea. Plant Cell Physiol.23: 1427–1433.
—,S.D. Kim, andJ.Y. Pak 1990. Effects of spermidine on chlorophyll content, photosynthetic activity and chlroplast ultrastructure in the dark and under light. Bot. Mag. Tokyo103: 43–48.
Spurr, A.R. 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruc. Res.26: 31–43.
Vanable, J.H. andR.A. Coggeshall 1965. A simplified lead citrate stain for use in electron microscopy. J. Cell Biol.25: 442–445.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pjon, CJ., Kim, YM. & Pak, JY. Interaction of cycloheximide and light on chlorophyll content and chloroplast ultrastructure inBrassica campestris . Bot Mag Tokyo 105, 681–685 (1992). https://doi.org/10.1007/BF02489442
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02489442