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Studies on several environmental factors for zygote formation and germination inClosterium ehrenbergii

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Abstract

Various conditions for zygote formation and germination inClosterium ehrenbergii were studied. HEPES buffered conjugation medium not only increased zygote formation, but also hastened germination. Zygote formation and germination depended largely on the state of cells before mating. Pre-culturing for 10 days was found optimal for zygote formation, while a 5 day or 10 to 20 day pre-culture was best suited for zygote germination. Interestingly, pre-culturing for 7 days resulted in a sudden drop in germination frequency. It is speculated that at 7 days some unknown factor necessary for germination becomes limiting in these cells. The length of mating period for 6 days gave the best results for zygote formation and germination. The frequency of germination increased proportionately until 60 days of maturation period in darkness after zygote formation.

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Author notes

  1. Jin Hamada

    Present address: Department of Microbial Genetics, The Research Institute for Microbial Diseases, Osaka University, Yamada-kami, 565, Suita-city, Osaka

Authors and Affiliations

  1. Laboratory of Genetics, Faculty of Agriculture, 606, Kyoto, Kyoto University

    Jin Hamada

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  1. Jin Hamada
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Contribution from the Laboratory of Genetics, No. 410.

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Hamada, J. Studies on several environmental factors for zygote formation and germination inClosterium ehrenbergii . Bot Mag Tokyo 91, 173–180 (1978). https://doi.org/10.1007/BF02489353

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  • DOI: https://doi.org/10.1007/BF02489353

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