Abstract
Multinucleate protoplasts were produced from meiotic cells at the zygotene and pachytene stages in a lily andTrillium, and their meiotic divisions were followed during subsequent culture. In each multinucleate, a complete synchrony of nuclear division was maintained throughout the meiotic process, and chromosome behavior appeared normal up to the metaphase stage. In most dinucleates, chromosome segregation movement was organized in a common spindle, and the daughter nuclei at the telophase appeared to envelope each other in the newly formed nuclear membrane. The cell was divided into two daughter cells by a common cell plate. Trinucleates were similarly converted to two cells with a hexaploid number of chromosomes. Some of the di- and trinucleates subsequently completed the second meiotic division with the formation of typical tetrad configurations. In giant cells with more than several nuclei, chromosomes separated at random but reaggregated into one giant resting nucleus, with no later cytokinesis. The rate of meiotic development in multinucleates was relatively slower in cells which contained greater numbers of nuclei.
Similar content being viewed by others
References
Bennett, M.D. 1973. The duration of meiosis. In: M. Balls and F.S. Billiett ed., The Cell Cycle in Development and Differentiation, 111–131 Cambridge Univ. Press.
— andJ.B. Smith. 1972. The effects of polyploidy on meiotic duration and pollen development in cereal anthers. Proc. Royal Soc. London, B181: 81–107.
Cocking, E.C. 1972. Plant cell protoplasts—Isolation and development. Ann. Rev. Plant Physiol.,23: 29–50.
Eriksson, T. andK. Jonasson. 1969. Nuclear division in isolated protoplasts from cells of higher plants grownin vitro. Planta80: 85–89.
Gamborg, O.L., K.N. Kao, R.A. Miller, L.C. Forke andF. Constabel. 1973. Cell regeneration, division and plant development from protoplasts. In: Protoplastes et Fusion de Cellules Somatiques Végétales. Edt. Inst. Natl. Recherche Agronomique, Paris 155–173.
Ito, M. 1973a. Studies on the behavior of meiotic protoplasts I. Isolation from microsporocytes of liliaceous plants. Bot. Mag. Tokyo86: 133–141.
— 1973b. Studies on the behavior of meiotic protoplasts II. Induction of a high fusion frequency in protoplasts from liliaceous plants. Plant & Cell Physiol.14: 865–872.
— andM. Maeda. 1973. Fusion of meiotic protoplasts in liliaceous plants. Exptl. Cell Res.80: 453–456.
— andH. Stern. 1967. Studies of meiosis in vitro I. In vitro culture of meiotic cells. Develop. Biol.16: 36–53.
Kao, K.N., O.L. Gamborg, M.R. Michayluk, W.A. Keller andR.A. Miller. 1973. The effects of sugars and inorganic salts on cell regeneration and sustained division in plant protoplasts. In: Protoplastes et Fusion de Cellules Somatiques Végétales. Edt. Inst. Natl. Recherche Agronomique, Paris 207–213.
— andM.R. Michayluk. 1974. A method for high-frequency intergeneric fusion of plant protoplasts. Planta115: 355–367.
Keller, W.A. andG. Melchers. 1973. The effect of high pH and calcium on tobacco leaf protoplast fusion. Zeitschr. Naturfor.28c: 737–741.
Miller, R.A., O.L. Gamborg, W.A. Keller andK.N. Kao. 1971. Fusion and division of nuclei in multinucleated soybean protoplasts. Can. J. Genet. Cytol.13: 347–353.
Motoyoshi, F. 1971. Protoplasts isolated from callus of maize endosperm. Formation of multinucleate protoplasts and nuclear division. Exptl. Cell. Res.68: 452–456.
Power, J.B. andE.C. Cocking. 1971. Fusion of plant protoplasts. Sci. Prog. Oxf.59: 181–198.
Reinert, J. andS. Hellmann. 1971. Mechanism of the formation of polynuclear protoplasts from cells of higher plants. Naturwissenschaften38: 419.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ito, M., Maeda, M. Meiotic division and fusion of nuclei in multinucleate cells from the induced fusion of meiotic protoplasts of liliaceous plants. Bot Mag Tokyo 87, 219–228 (1974). https://doi.org/10.1007/BF02489298
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02489298