Biosystematic studies of theClosterium peracerosum-strigosum-littorale complex

II. Reproductive isolation and morphological variation among several populations from the Northern Kanto Area in Japan
  • Makoto M. Watanabe
  • Terunobu Ichimura


Nine representative pairs of heterothallic (=self-sterile, cross-fertile) strains of theClosterium peracerosum-strigosum-littorale complex from the northern Kanto area in Japan have been studied under the defined standard culture conditions. Since a wall thickening at cell apices was observed in vegetative cells of all the strains, these strains turned out to belong to the morphological group II (Ichimura and Watanabe, 1976). The result of statistical analyses of their cell size variations corresponded well with the result of intercrossing experiments between them. It was shown that these strains are virtually composed of three biologically different groups which are morphologically distinct and reproductively isolated completely or at least partially from each other. For convenience, these three groups have been designated as II-A, II-B, and II-C in the order of from smaller to larger cell size. In intra-group crossings, a large number of zygospores were formed, and they germinated well to yield healthy populations of their progenies in all the three groups. In inter-group crossings, no sign of sexual reproduction was observed between Group II-A and Group II-C or Group II-B and Group II-C, and a marked decrease of zygospore formation was observed between Group II-A and Group II-B, especially between Group II-A minus and Group II-B plus. It was concluded that the distinctions between the three groups are biologically sound and that each represents an evolutionary unit.


Paddy Field Reproductive Isolation Compatible Pair Biosystematic Study Sexual Reaction 
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Copyright information

© The Botanical Society of Japan 1978

Authors and Affiliations

  • Makoto M. Watanabe
    • 1
    • 2
  • Terunobu Ichimura
    • 1
    • 2
  1. 1.Department of Botany, Faculty of ScienceHokkaido UniversitySapporo
  2. 2.Institute of Applied MicrobiologyUniversity of TokyoTokyo

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