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Research review: DNA polymerases as molecular markers of the regenerating capacity of hepatocytes

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Journal of Hepato-Biliary-Pancreatic Surgery

Abstract

Hepatocyte regeneration has been widely investigated, with the mitotic index and the incorporation of [3H]thymidine being used as regeneration markers. We focused on the induction of DNA replication enzymes, particularly DNA polymerases (pol) α, δ, and ε. Using rat models, we have shown that the activity of pol α in crude liver extract well represents the regenerating capacity of hepatocytes. Using pol α as an indicator, we analyzed liver regeneration in rat models under various conditions: obstructive jaundice, external or internal biliary drainage, and the obstruction of portal vein branches. It has been revealed that the ligation of the common bile duct alone induces a certain amount of hepatocyte proliferation. It was striking that external biliary drainage suppressed regeneration capacity in cholestatic rat liver after partial hepatectomy. The strong regeneration in nonligated lobes induced by portal branch ligation was similar to the liver regeneration seen after partial hepatectomy with respect to the induction of DNA polymerases. Taken together, the aspects of DNA replication, particularly the induction of DNA polymerases, may contribute to shedding new light on the regeneration of human liver.

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Authors and Affiliations

  1. First Department of Surgery, Research Institute for Disease Mechanism and Control, Nagoya, University School of Medicine, 65 Tsurumai-cho, Syowa-ku, 466, Nagoya, Japan

    Eiji Takeuchi, Yuji Nimura, Shin-ichi Mizuno, Hideaki Suzuki, Shinsuke Iyomasa, Masaki Terasaki & Hiroshi Kuriki

  2. Laboratory of Cancer Cell Biology, Research Institute for Disease Mechanism and Control, Nagoya, University School of Medicine, 65 Tsurumai-cho, Syowa-ku, 466, Nagoya, Japan

    Keiko Tamiya-Koizumi & Shonen Yoshida

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  1. Eiji Takeuchi
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  2. Yuji Nimura
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  3. Shin-ichi Mizuno
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  4. Hideaki Suzuki
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  5. Shinsuke Iyomasa
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  6. Masaki Terasaki
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  7. Hiroshi Kuriki
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  8. Keiko Tamiya-Koizumi
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  9. Shonen Yoshida
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This work was supported in part by a Grant-in-Aid for General Scientific Research and for Cancer Research from the Ministry of Education, Science and Culture, Japan, and by grants from the Uehara Memorial Foundation

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Takeuchi, E., Nimura, Y., Mizuno, Si. et al. Research review: DNA polymerases as molecular markers of the regenerating capacity of hepatocytes. J Hep Bil Pancr Surg 4, 254–262 (1997). https://doi.org/10.1007/BF02489022

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  • DOI: https://doi.org/10.1007/BF02489022

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