Cytogenetic analysis of gallbladder neoplasms using fluorescence in situ hybridization (FISH)
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To characterize the numerical chromosome aberrations in gallbladder neoplasms, we examined surgically resected tissues using fluorescence in situ hybridization. The aberrations in 15 specimens of adenocarcinomas and 2 adenomas were compared with those in 4 samples of adenomyomatosis and 17 samples of normal epithelium. We calculated the frequency of aneusomy and determined the chromosome indexes (mean number of chromosomes per nucleus) of chromosomes 17 and 18. The pattern of DNA ploidy was analyzed by flow cytometry. In normal epithelium, adenomyomatosis and adenomas, DNA aneuploidy was not observed, while 13 (87%) carcinomas showed DNA aneuploidy, including 2 specimens with multiploidy. No numerical aberrations were observed in normal epithelium and adenomyomatosis. A numerical gain of chromosome 17 was observed in a single adenoma and in 10 (66%) carcinomas. A numerical gain of chromosome 18 was observed in 6 (40%) carcinomas, but not in other tissues. The chromosome index of chromosome 17 was significantly higher in adenomas and carcinomas (2.45±0.60 and 2.29±0.14, respectively) compared with normal epithelium. Our cytogenetic findings did not correlate with any histopathologic features of carcinomas. Our results indicated that the gains of chromosome 17 and 18 represented early chromosomal alterations in gallbladder neoplasms and were maintained in advanced carcinomas.
Key wordsnumerical chromosome aberrations chromosome 17 chromosome 18 DNA ploidy
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- 1.Tsukuma H (1992) Incidence of cancer prediction in Japan up to the year 2015. Jpn J Cancer Clin 38:1–10Google Scholar
- 4.Noravud N, Foster CS, Gilbertson JA, Silora K, Waxman J (1989) Oncogene expression in cholangiocarcinoma and in normal hepatic development. Hum Pathol 20:1163–1168Google Scholar
- 10.Jibiki M (1994) Study of nuclear DNA content and chromosomal numerical aberrations using fluorescence in situ hybridization in colorectal polyps and colorectal adenomas. Acta Med Nagasaki 39:100–105Google Scholar
- 13.Nanashima A, Tagawa Y, Yasutake T, Sawai T, Tuji T, Sasano O, Nakagoe T, Ayabe H (1997) Aneusomy of chromosome 18 is associated with the development and progression of colorectal carcinoma. Jpn J Gastroenterol (in press)Google Scholar
- 14.Yasutake T (1990) Surgical application of DNA ploidy to nonsmall-cell lung carcinoma. Acta Med Nagasaki 35:145–151Google Scholar
- 17.Japanese Society of Biliary Surgery (1993) General rules for surgical and pathological studies on cancer of biliary tract, 3rd edn. Kanehara, TokyoGoogle Scholar
- 21.Yamaguchi H (1992) Analysis of numerical chromosome aberration of gastric cancer: Application of fluorescence in situ hybridization using chromosome-specific DNA probes. Acta Med Nagasaki 37:163–170Google Scholar
- 23.Morinaga M, Tagawa Y, Yasutake T, Miyashita K, Sawai T, Matsumoto Y, Nanashima A, Hatano K, Uchikawa T, Fujise N, Taniguchi Y, Nishizawa JE, Matsuo S, Nakagoe T, Ayabe H, Tomita M (1994) Detection of chromosomal numerical aberration in early colorectal carcinomas using fluorescence in situ hybridization. Jpn J Cancer Chemother 21 (Suppl I):75–81Google Scholar
- 26.Crocker J (1990) Nucleolar organiser regions. In: James CE Underwood (ed) Pathology of the nucleus. Springer Berlin Heidelberg New York London Paris Tokyo Hong Kong, pp 92–149Google Scholar