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NAD-dependent shikimate dehydrogenase from mung bean cells in suspension culture

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Abstract

Shikimate dehydrogenase in mung bean cell suspension culture was partially purified by chromatography on DEAE-cellulose. The NADP-dependent SDHase was eluted as a single peak, while the NAD-dependent one exhibited two peaks. Polyacrylamide gel electrophoresis revealed six NAD-dependent isozymes of SDHase, two of which corresponded to NADP-dependent ones.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

DTT:

dithiothreitol

MTT:

3(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide

PMS:

phenazine methosulfate

SDH:

shikimate dehydrogenase

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Authors and Affiliations

  1. Department of Biology, Japan Women's University, Mejirodai, 112, Tokyo

    Kyoko Ogawa & Taka N. Tateoka

Authors
  1. Kyoko Ogawa
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  2. Taka N. Tateoka
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Ogawa, K., Tateoka, T.N. NAD-dependent shikimate dehydrogenase from mung bean cells in suspension culture. Bot Mag Tokyo 101, 203–206 (1988). https://doi.org/10.1007/BF02488896

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  • DOI: https://doi.org/10.1007/BF02488896

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