Abstract
In order to clarify the regulatory mechanism of gene expression of plasmid pDG1 from a wild isolateDictyostelium sp. GA11, we introduced pDG1 gene into the laboratory strainDictyostelium discoideum AX3K using the integration vector, B10SX. Three transformants contained about 100 copies of pDG1 gene per cell. Several different sized trancripts of pDG1 gene were observed, suggesting that inD. discoideum pDG1 gene may not be transcribed in the precise manner as in its original host strain, GA11. Nevertheless, developmental regulation of pDG1 gene expression inD. discoideum was shown to be similar to that in GA11. In addition, cAMP and its analogue, 2′-deoxy cAMP increase the accumulation of pDG1 transcripts in transformedD. discoideum cells. These results are discussed in connection with the possible regulatory mechanism of gene expression in pDG1.
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Orii, H., Tanaka, Y. & Yanagisawa, K. Developmentally and cAMP regulated gene expression of the plasmid pDG1 inDictyostelium discoideum transformant. Bot Mag Tokyo 101, 163–173 (1988). https://doi.org/10.1007/BF02488893
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DOI: https://doi.org/10.1007/BF02488893