The botanical magazine = Shokubutsu-gaku-zasshi

, Volume 98, Issue 3, pp 219–223 | Cite as

Differential distribution of ascorbic acid and RNA in the developing anthers ofDatura stramonium L.

  • Ravi R. Hegde


The histochemical localization of ascorbic acid and RNA was studied during developmental stages ofDatura anthers. The concentration of ascorbic acid and RNA was high in primary parietal and primary sporogenous layers, sporogenous cells and pollen grains. The connective of young anther showed remarkably high concentration of ascorbic acid. The high peaks of ascorbic acid and RNA concentration correlated with the growth phases of anther. The connective and anther wall layers act as reservoirs of energy needed for developing sporogenous cells.

Key words

Ascorbic acid Datura anther RNA 


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  1. Andrade, L. andR.R. Hegde. 1983. Distributional pattern of general polysaccharides, starch and some respiratory enzymes in developingDatura anther. Karnatak Univ. J. (Sci.)28: 33–39.Google Scholar
  2. Chinoy, J.J. 1970. Heredity in terms of reaction rates and electron transport. Indian J. Plant Physiol.13: 115–158.Google Scholar
  3. —,Y.D. Singh andK. Gurumurti. 1971. Some aspects of the physiological role of ascorbic acid in plants. Indian Agric.15: 33–48.Google Scholar
  4. Chinoy, N.J. 1969. On the specificity of the alcoholic acidic silver nitrate for the histochemical localization of ascorbic acid. Histochemie20: 105–107.PubMedCrossRefGoogle Scholar
  5. Erickson, R.O., K.B. Sax andM. Ogur. 1949. Perchloric acid in the cytochemistry of pentose nucleic acid. Science110: 472–473.PubMedGoogle Scholar
  6. Flax, M.H. andM.H. Himes. 1952. Microspectrophotometric analysis of metachromatic staining of nucleic acids. Physiol. Zool.25: 297–311.Google Scholar
  7. Hegde, R.R. andL. Andrade. 1982. Anther development inDatura: Distribution of proteins, esterase and adenosine triphosphatase. Plant Sci. Letters28: 95–101.Google Scholar
  8. Hegde, R.R. and L. Andrade. 1984. Histochemical studies on developingDatura anther: Distributional pattern of lipids and lipase. Proc. 71st Ind. Sci. Cong. Abst. p. 110.Google Scholar
  9. Heslop-Harrison, J. 1972. Sexuality of Angiosperms.In: F.C. Steward, ed., Plant Physiology6C: 134–289. Academic Press New York.Google Scholar
  10. Madhavan Unni, N.V. andC.K. Shah. 1968. Ascorbic acid localization during vegetative and reproductive differentiation inCyperus rotundus L. Indian J. Plant Physiol.11: 172–180.Google Scholar
  11. Poddubnaya-Arnoldi, V.A. andN.V. Zinger. 1959. Histochemical study of the pollen grains and pollen tubes in the angiosperms. Recent Adv. Bot.1: 682–685.Google Scholar
  12. Rudramuniyappa, C.K. andM.G. Panchaksharappa. 1980. Pollen development inTriticum durum Desf.: A histochemical study. J. S. Afr. Bot.46: 33–43.Google Scholar
  13. Schopfer, P. 1967. Further investigation on the phytochrome-mediated accumulation of ascorbic acid in the mustard seedlings (Sinapis alba L.). Planta74: 210–227.CrossRefGoogle Scholar
  14. Shah, C.K. andP.N. Bhatt. 1977. Histochemical localization of ascorbic acid, RNA and histones in anthers ofCoix.In: V.R. Dnyansagaret al., ed., Recent Trends and ontacts between Cytogenetics, Embryology and Morphology. p. 527–537. Today and Tomorrow's Print. & Publ., New Delhi.Google Scholar
  15. —,S.N. Pandey andP.N. Bhatt. 1974. Cytophotometric study of ascorbic acid in the developing anthers ofAponogeton natans L. Indian J. Plant Physiol.17: 16–22.Google Scholar

Copyright information

© The Botanical Society of Japan 1985

Authors and Affiliations

  • Ravi R. Hegde
    • 1
  1. 1.Histochemistry and Morphogenesis Laboratory, Department of BotanyKarnatak UniversityKarnatakaIndia

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