Abstract
The presence of an enzyme involved in ATP synthesis by transfer of phosphate from thiamine triphosphate to ADP in maize germ axis was indicated by the assays on partially purified (27-fold) enzyme with luciferase method, spectrophotometric assay with hexokinase and glucose-6-phosphate dehydrogenase, and paper chromatography. Optimal activity was found at pH 9.0. The enzyme was heat-labile SH-enzyme, and its activity required the presence of Mg2+.
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Abbreviations
- DTT:
-
dithiothreitol
- Gl-6-P:
-
glucose-6-phosphate
- PCMB:
-
p-chloromercuribenzoate
- TDP:
-
thiamine diphosphate
- TTP:
-
thiamine triphosphate
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Kikuchi, M., Ikawa, T. Presence of an enzyme mediating transfer of phosphate from thiamine triphosphate to ADP in germinating maize. Bot Mag Tokyo 97, 193–205 (1984). https://doi.org/10.1007/BF02488693
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DOI: https://doi.org/10.1007/BF02488693