The botanical magazine = Shokubutsu-gaku-zasshi

, Volume 94, Issue 3, pp 239–247 | Cite as

Giant oil cells in the cotyledons ofPharbitis nil and other convolvulacean plants

  • Kiyomi Wada
  • Akeo Kadota
  • Hiromichi Tanihira
  • Yoshihiro Suzuki
Article
  • 52 Downloads

Abstract

Suspension of protoplasts (ca. 13–25 μm in diameter) that were isolated from the mesophyll of the cotyledons ofPharbitis nil, strain Violet, contained many large spherical or spheroidal bodies (ca. 100 μm in diameter). Microscopic observation of these bodies and some anatomic studies of the cotyledons during embryogenesis and after germination showed that these bodies are giant cells containing many oil drops stainable with Sudan dyes. Such giant cells were found in four otherPharbitis nil strains, Nepal, Tendan, Africa and Tokyo-kokei, and in six other Convolvulacean plants,Ipomoea batatas, cv. Koukei-14,Calystegia japonica, Calystegia hederacea, Calonyction aculeatum, Quamoclit pennata andCuscuta japonica.

Key words

Cell preparation Convolvulacean seeds Embryogenesis Oil cell Pharbitis cotyledons 

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References

  1. Hallier, H. 1893. Versuch einer natürlichen Gliederung der Convolvulaceen auf morphologischer und anatomischer Grundlage. Engler's bot. Jahrb.56: 454–591.Google Scholar
  2. Harz, C.O. 1885. Samenkunde2: 752–754. Berlin.Google Scholar
  3. Hayward, H.E. 1932. The seedling anatomy ofIpomoea batatas. Bot. Gaz.93: 400–420.CrossRefGoogle Scholar
  4. Hyrano, K. 1888. Ein Beitrag zur Kenntniss der Samen vonPharbitis triloba Meia. Mitteil. Medizin. Fak. Kaiser. Univ., Tokyo.1: 201–208.Google Scholar
  5. Matsubara, S., andR. Nakahira. 1966.In vitro formation of adventitious organs byPharbitis embryos. Nature211: 1208–1210.CrossRefGoogle Scholar
  6. Messerschmidt, M. 1974. Kallusbildung und Differenzierung aus isolierten Protoplasten vonPharbitis nil. Z. Pflanzenphysiol.74: 175–178.Google Scholar
  7. Ogawa, Y. 1963. Gibberellin-like substances occurring in the seed ofPharbitis nil Chois. and their change in contents during the seed development. Plant Cell Physiol.4: 217–225.Google Scholar
  8. Popham, R.A., T.J. Johnson andA.P. Chan. 1948. Safranin and aniline blue with Delafield's hematoxylin for staining cell walls in shoot apexes. Stain Technol.23: 185–190.Google Scholar
  9. Solereder, H. 1899. Systematische Anatomie der Dicotyledonen p. 635–649. Verlag von Ferdinand Enke, Stuttgart.Google Scholar
  10. Ueda, R. andR. Aimi. 1964. Shokubutsu-keitaigaku; Shokubutsu-seirigaku p. 126. Iwasaki Books Co. Ltd., Tokyo (in Japanese).Google Scholar
  11. Zacharias, E. 1879. Ueber Secret-Behälter mit verkorkten Membranen. Bot. Zeitschr.37: 633–645.Google Scholar

Copyright information

© The Botanical Society of Japan 1981

Authors and Affiliations

  • Kiyomi Wada
    • 1
  • Akeo Kadota
    • 1
  • Hiromichi Tanihira
    • 1
  • Yoshihiro Suzuki
    • 1
  1. 1.Biological Institute, Faculty of ScienceShizuoka UniversityShizuoka

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