Abstract
To reveal the behavior of silver stainable material localized mainly in the nucleoli and nucleolar organizing regions (NORs), the somatic cells ofVicia faba were investigated by silver staining throughout the mitotic cell cycle. Nucleoli of interphase and early prophase nuclei were darkly stained. From late prophase to anaphase the secondary constrictions were discriminated as silver stained NORs and many silver grains appeared throughout the cytoplasm. At late prophase the NOR condensed at the same rate as the chromosome arm. Small spherical bodies and two new nucleoli appeared in telophase nuclei and at the same time the cytoplasmic grains disappeared. On the basis of the above observations on the silver stainable material during each mitotic phase, the behavior of silver stainable material is interpreted.
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References
Burger, E.-C. andM. Knälmann. 1980. Koinzidenz von Feulgen-Achromasie, in situ Hybridisierung und Silberbandenfärbung in vier Nukleolusorganisatoren vonVicia sativa. Eur. J. Cell Biol.24: 313–318.
Fuys, C.H.C.M. andJ. Osinga. 1980. Abundance of protein-bound sulfhydryl and disulfide groups at chromosomal nucleolus organizing regions. Chromosoma77: 1–11.
Das, N.K. 1962. Demonstration of a non-RNA nucleolar fraction by silver staining. Exp. Cell Res.26: 428–431.
Giménez-Martín, G., M.E. Fernández-Gómez, A. González-Fernández andC. De la Torre. 1971. The nucleolar cycle in meristematic cells. Cytobiologie4: 330–338.
Goodpasture C. andS.E. Bloom. 1975. Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining. Chromosoma53: 37–50.
Hizume, M., S. Sato andA. Tanaka 1980a. A highly reproducible method of nucleolus organizing regions staining in plants. Stain Technol.55: 87–90.
— andR. Tanaka. 1980b. A technique for C banding inVicia faba chromosomes. Jap. J. Genet.55: 301–305.
Howell, W.M. 1977. Visualization of ribosomal gene activity: silver stains protein associated with rRNA transcribed from oocyte chromosomes. Chromosoma62: 361–367.
— andJ.R. Diamond. 1975. Differential staining of the satellite regions of human acrocentric chromosomes. Experientia31: 260–262.
Hubbell, H.R., Y. Lau, R.L. Brown andT.C. Hsu. 1980. Cell cycle analysis and during inhibition studies of silver staining in synchronous HeLa cells. Exp. Cell Res.129: 139–147.
— andT.C. Hsu. 1979. Identification of silver binding protein associated with the cytological silver staining of actively transcribing nucleolar regions. Cell Biol. Int. Rep.3: 615–622.
Lafontaine, J.G. andL.A. Chouinard. 1963. A correlated light and electron microscope study of the nucleolar material during mitosis inVicia faba. J. Cell Biol.17: 167–201.
Miller, D.A., V.G. Dev, R. Tantravahi andO.J. Miller. 1976. Suppression of human nucleolus organizer activity in mouse-human somatic hybrid cells. Exp. Cell Res.101: 235–243.
Pelliccia, F., A. de Capoa, G. Belloni, A. Rocchi andM. Ferraro. 1978. Localization of silver staining in interphase, prohase and metaphase lymphocytes. Exp. Cell Res.115: 439–441.
Sato, S., M. Hizume andS. Kawamura. 1980. Relationship between secondary constrictions and nucleolus organizing regions inAlliumssativum chromosomes. Protoplasma105: 77–85.
Schubert, I., M. Anastassova-Kristeva andR. Rieger. 1979. Specificity of NOR staining inVicia faba. Exp. Cell Res.120: 433–435.
Schwarzacher, H.G., A.-V. Mikelsaar andW. Schnedl. 1978. The nature of the Agstaining of nucleolus organizer regions. Cytogenet. Cell Genet.20: 24–39.
Schweizer, D. andP. Ambros. 1979. Analysis of nucleolus organizer regions (NORs) in mitotic and polyten chromosomes ofPhaseolus coccineus by silver staining and Giemsa C-banding. Plant Syst. Evol.132: 27–51.
Stevens, B.J. andD.M. Prescott. 1971. Reformation of nucleolus-like bodies in the absence of postmitotic RNA synthesis. J. Cell Biol.48: 443–454.
Stokert, J.C., M.E. Fernández-Gómez, G. Giménez-Martín andJ.F. López-Sáez. 1970. Organization of argyrophylic nucleolar material throughout the division cycle of meristematic cells. Protoplasma69: 267–278.
Tandler, C.J.. 1959. The silver-reducing property of the nucleolus and the formation of prenucleolar material during mitosis. Exp. Cell. Res.17: 560–564.
von Kalm, L. andD.R. Smyth. 1980. Silver staining test of nucleolar suppression in theLilium hybrid “Black Beauty”. Exp. Cell Res.129: 481–485.
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Hizume, M., Tanaka, A. & Miyata, Y. Behavior of silver stainable material during mitosis inVicia faba . Bot Mag Tokyo 95, 81–87 (1982). https://doi.org/10.1007/BF02488575
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DOI: https://doi.org/10.1007/BF02488575