The botanical magazine = Shokubutsu-gaku-zasshi

, Volume 95, Issue 3, pp 331–338 | Cite as

Requirement of Ca2+ for the gelatinization of pectin solution by a proteinous cell wall-bound factor and solubilization of the gel by acid treatment

  • Tsuyoshi Yamaoka


The cell wall-bound pectin-gelatinizing factor, whose existence was reported in a previous paper (Yamaoka and Sato, 1981), was found to be composed of a proteinous factor (PGF) and calcium ion. Magnesium ion could not replace Ca2+ whereas Sr2+ could replace Ca2+ and Ba2+ could cause gelation without the action of PGF. In the hypocotyl of soybean seedlings, the basal region contained more PGF than did the apical region. The gel of apple pectin formed by the action of PGF and Ca2+ was solubilized by acid treatment for some time after the gelation. It was also found that the gel became more and more resistant to acidity with time. The hardened gel was almost completely dissolved by boiling in 0.2 M NaCl solution. The idea that the gelation of apple pectinin vitro may simulate the gelation of pectin in the primary cell wall was discussed in view of the results and the phenomenon of acid growth.

Key words

Acid growth Barium ion Calcium ion Cell wall Pectin gel Strontium ion 



disodium ethylenediaminetetraacetate


pectin-gelatinizing factor


pectin methylesterase


stoke (a unit of kinematic viscosity, cm2·sec−1)


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Copyright information

© The Botanical Society of Japan 1982

Authors and Affiliations

  • Tsuyoshi Yamaoka
    • 1
  1. 1.Laboratory of Science Education Research, Faculty of EducationAkita UniversityAkita City, Akita

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