Abstract
A method for the enzymatic isolation of meiotic protoplasts from two species of liliaceous plants is described. Protoplasts can be produced from each meiotic stage with more than 70% survival. If produced at prophase, most of them can be cultured through the meiotic cycle after cell-wall regeneration.
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Ito, M. Studies on the behavior of meiotic protoplasts. Bot Mag Tokyo 86, 133–141 (1973). https://doi.org/10.1007/BF02488521
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DOI: https://doi.org/10.1007/BF02488521