Protein synthesis in the embryo ofPinus thunbergii seed III.

Abnormal dissociation of the ribosomes from the dry (Non-imbined) seed embryos
  • Naoki Yamamoto
  • Satohiko Sasaki


Dissociability of the monomer ribosomes prepared from dry and imbibed pine (Pinus thunbergii) seed embryos was analyzed in sucrose density gradient containing a high salt buffer. Abnormal dissociation into the subunits was observed with the ribosome preparation from dry seed embryos when compared with that from imbibed seed embryos, i.e. each subunit peak was broader and localized at a lower site in sucrose density gradient. This indicates some change(s) in ribosomes during imbibition of seeds. These ribosomal changes also progressedin vitro. That is, after incubation of ribosome preparation from dry seed embryos in a high salt buffer for 5 min at 30 C or in a low salt buffer for 15 hr at 0 C, complete dissociation into the normal subunits was observed. No difference was found between polyacrylamide gel electrophoresis patterns of ribosomal RNA from dry and imbibed seed embryos. These results suggest some alteration in the protein components of ribosome during imbibition of pine seeds.


Sucrose Density Gradient Salt Buffer Seed Embryo Sucrose Density Gradient Centrifuga High Salt Buffer 
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N-2-hydroxyethyl-piperazine-N′-2-ethanesulfonic acid




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Copyright information

© The Botanical Society of Japan 1979

Authors and Affiliations

  • Naoki Yamamoto
    • 1
    • 2
  • Satohiko Sasaki
    • 1
    • 2
  1. 1.Department of Biology, Faculty of EducationUtsunomiya UniversityUtsunomiya
  2. 2.Forestry and Forest Products Research InstituteIbaraki

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