Advertisement

Protein synthesis in the embryo ofPinus thunbergii seed III.

Abnormal dissociation of the ribosomes from the dry (Non-imbined) seed embryos
  • Naoki Yamamoto
  • Satohiko Sasaki
Article

Abstract

Dissociability of the monomer ribosomes prepared from dry and imbibed pine (Pinus thunbergii) seed embryos was analyzed in sucrose density gradient containing a high salt buffer. Abnormal dissociation into the subunits was observed with the ribosome preparation from dry seed embryos when compared with that from imbibed seed embryos, i.e. each subunit peak was broader and localized at a lower site in sucrose density gradient. This indicates some change(s) in ribosomes during imbibition of seeds. These ribosomal changes also progressedin vitro. That is, after incubation of ribosome preparation from dry seed embryos in a high salt buffer for 5 min at 30 C or in a low salt buffer for 15 hr at 0 C, complete dissociation into the normal subunits was observed. No difference was found between polyacrylamide gel electrophoresis patterns of ribosomal RNA from dry and imbibed seed embryos. These results suggest some alteration in the protein components of ribosome during imbibition of pine seeds.

Keywords

Sucrose Density Gradient Salt Buffer Seed Embryo Sucrose Density Gradient Centrifuga High Salt Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

HEPES

N-2-hydroxyethyl-piperazine-N′-2-ethanesulfonic acid

DTT

dithiothreitol

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. App, A.A., M.G. Bulis andW.J. McCarthy. 1971. Dissociation of ribosomes and seed germination. Plant Physiol.47: 81–86.PubMedGoogle Scholar
  2. Beller, R.J. andB.D. Davis. 1971. Selective dissociation of free ribosomes ofEscherichia coli by sodium ions. J. Mol. Biol.55: 477–485.PubMedCrossRefGoogle Scholar
  3. Gordon, M.E. andP.I. Payne. 1976. In vitro translation of the long-lived messenger ribonucleic acid of dry seeds. Planta130: 269–273.CrossRefGoogle Scholar
  4. Kobayashi, Y. andH.O. Harvorson. 1968. Evidence for a defective protein synthesizing system in dormant spores ofBacillus cereus. Arch. Biochem. Biophys.123: 622–632.PubMedCrossRefGoogle Scholar
  5. Loening, U.E. andJ. Ingle. 1967. Diversity of RNA components in green plant tissues. Nature215: 363–367.PubMedCrossRefGoogle Scholar
  6. Sasaki S. andS. Asakawa. 1974. Effect of monochromatic lights on the germination of pine seeds processed in darkness. J. Jap. For. Soc.56: 361–365.Google Scholar
  7. Shultz, G.A., D. Chen andE. Katchaski. 1972. Localization of a mRNA in a ribosomal fraction from ungerminated wheat embryos. J. Mol. Biol.66: 379–390.CrossRefGoogle Scholar
  8. Spiegel, S. andA. Marcus. 1975. Polyribosome formation in early wheat embryo germination independent of either transcription or polyadenylation. Nature256: 228–230.CrossRefGoogle Scholar
  9. Travis, R.L., C.Y. Lin andJ.L. Key. 1972. Enhancement by light of thein vitro protein synthetic activity of cytoplasmic ribosomes isolated from dark-grown maize seedlings. Biochim. Biophys. Acta277: 606–614.PubMedGoogle Scholar
  10. Yamamoto, N. 1977. Protein synthesis in the embryo ofPinus thunbergii seed I. Influences of imbibition of seeds in the dark. Plant Cell Physiol.18: 287–292.Google Scholar
  11. —,M. Hasegawa, S. Sasaki andS. Asakawa. 1975. Red-far red reversible effect on polysome formation in the embryos ofPinus thunbergii seeds. Plant Physiol.56: 734–737.PubMedCrossRefGoogle Scholar
  12. —,S. Sasaki, S. Asakawa andM. Hasegawa. 1974. Polysome formation induced by light inPinus thunbergii seed embryos. Plant Cell Physiol.15: 1143–1146.Google Scholar
  13. Zylber, E.A. andS. Penman. 1970. The effect of high ionic strength on monomer, polyribosomes, and puromycin-treated polyribosomes. Biochim. Biophys. Acta204: 221–229.PubMedGoogle Scholar

Copyright information

© The Botanical Society of Japan 1979

Authors and Affiliations

  • Naoki Yamamoto
    • 1
    • 2
  • Satohiko Sasaki
    • 1
    • 2
  1. 1.Department of Biology, Faculty of EducationUtsunomiya UniversityUtsunomiya
  2. 2.Forestry and Forest Products Research InstituteIbaraki

Personalised recommendations