Abstract
Effects of some chemicals on the activities of two ribonuclease isozymes, M and F, in the leaves ofNicotiana glutinosa were investigated. CHX and AMD suppressed the increase of M isozyme activity induced with mock-inoculation. On the other hand, CHX suppressed slightly the increase of F izozyme activity induced with TMV-inoculation, but AMD and 5-FU stimulated it.
Abbreviations
- TMV:
-
tobacco mosaic virus
- CHX:
-
cycloheximide
- AMD:
-
actinomycin D
- 5-FU:
-
5-fluorouracil
References
Baumgartner, B., H. Kende andP. Matile. 1975. Ribonuclease in senescing morning glory: purification and demonstration ofde novo synthesis. Plant Physiol.55: 734–737.
Datta, K., L. Marsh andA. Marcus. 1983. Early growth of wheat embryonic axes and the synthesis of RNA and DNA. Plant Physiol.72: 394–397.
Farkas, G.L. 1968. A comparison of biochemical changes associated with virus-induced lesion formation and mechanical injury.In T. Hirai, Z. Hidaka and I. Uritani, ed., Biochemical Regulation in Diseased Plants or Injury, pp. 123–135. Kyoritsu Printing Co., Tokyo.
Frisch-Niggemeyer, W. andK.K. Reddi. 1957. Studies on ribonuclease in tobacco leaves: I. purification and properties. Biochim. Biophys. Acta26: 40–46.
Matsushita, K. andH. Shimokawa. 1983. Behaviors of ribonuclease in tobacco mosaic virus-infectedNicotiana glutinosa leaves. Ann. Phytopath. Soc. Japan49: 206–213.
Reddi, K.K. 1963. Studies on the formation of tobacco mosaic virus ribonucleic acid: II. Degradation of host ribonucleic acid following infection. Proc. Natl. Acad. Sci. USA.50: 75–81.
Sacher, J.A., J. Tseng, R. Williams andA. Cabello. 1982. Wound-induced RNase activity in sweet potato: evidence for regulation at transcription. Plant Physiol.69: 1060–1065.
Shimokawa, H., R.T. Itoh, M. Sanada andK. Matsushita. 1987. Isolation and characterization of the RNase isozymes ofNicotiana glutinosa leaves infected with tobacco mosaic virus. Nature and Culture No.14: 67–71. (The Bulletin of the Liberal Arts Course of Fukuoka Dental College).
Shinde, B.G. andV. Santilli. 1967. Effect of actinomycin D on the tobacco mosaic virus infection-induced increase in ribonuclease activity. Phytopathology57: 345.
Takaiwa, F. andS. Tanifuji. 1978. Development of RNase activity in embryonic axes of germinating pea seeds. Plant Cell Physiol.19: 1507–1518.
Takimoto, C.H., Y.Y. Tan, E.C. Cadman andR.D. Armstrong. 1987. Correlation between ribosomal RNA production and RNA-directed fluoropyrimidine cytotoxicity. Biochem. Pharmacol.36: 3243–3248.
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Shimokawa, H., Itoh, R.Ti. & Matsushita, K. A comparative study of two RNase isozyme activities inNicotiana glutinosa leaves. Bot. Mag. Tokyo 102, 457–461 (1989). https://doi.org/10.1007/BF02488127
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DOI: https://doi.org/10.1007/BF02488127