Skip to main content
Log in

Peculiar spindle configuration in the pollen tube revealed by the anti-tubulin immunofluorescence method

  • Short Communication
  • Published:
The botanical magazine = Shokubutsu-gaku-zasshi Aims and scope Submit manuscript

Abstract

The spindles in generative cell divisions within the pollen tubes ofCalanthe andImpatiens were revealed by anti-α-tubulin immunofluorescence methods. They were peculiar configurations in which the metaphase chromosomes lay tandemly in some lines along the spindle axis and the sister chromosomes separated anti-parallelly by the spindle elongation during anaphase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Brewbaker, J. andB.H. Kwack. 1963. The essential role of calcium ion in pollen germination and pollen tube growth. Amer. J. Bot.35: 859–865.

    Article  Google Scholar 

  • Burgess, J. 1970. Cell shape and mitotic spindle formation in the generative cell ofEndymion non-scriptus. Planta (Berl.)95: 72–85.

    Google Scholar 

  • Cresti, M., F. Ciampolini andR.N. Kapil. 1984. Generative cells of some angiosperms with particular emphasis on their microtubules. J. Submicrosc. Cytol.16: 317–326.

    Google Scholar 

  • Derksen, J., E.S. Pierson andJ.A. Traas. 1985. Microtubules in vegetative and generative cells of pollen tubes. Eur. J. Cell Biol.38: 142–148.

    Google Scholar 

  • Johnston, G.W. 1941. Cytological studies of male gamete formation in certain angiosperms. Amer. J. Bot.28: 306–319.

    Article  Google Scholar 

  • Raghavan, T.S., K.R. Venkatasubbaan andH.D. Wulff. 1939. Division of the generative cell inImpatiens Balsamina L. Cytologia9: 389–392.

    Google Scholar 

  • Sax, K. andJ.G. O'Mara. 1941. Mechanism of mitosis in pollen tubes. Bot. Gaz.102: 629–636.

    Article  Google Scholar 

  • Terasaka, O. andR. Tanaka. 1974. Absence of the nucleolar constriction in the division of generative nucleus in the pollen of some angiosperms. Cytologia39: 97–106.

    Google Scholar 

  • Wick, S.M., R.W. Seagull, M. Osborn, K. Weber andB.E.S. Gunning. 1981. Immunofluorescence microscopy of organized microtubule arrays in structurally stabilized meristematic plant cells. J. Cell Biol.89: 685–690.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Terasaka, O., Niitsu, T. Peculiar spindle configuration in the pollen tube revealed by the anti-tubulin immunofluorescence method. Bot. Mag. Tokyo 102, 143–147 (1989). https://doi.org/10.1007/BF02488119

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02488119

Key words

Navigation