European Archives of Oto-Rhino-Laryngology

, Volume 252, Supplement 1, pp S40–S43 | Cite as

Concentrations of chemical mediators in nasal secretions after nasal allergen challenges in atopic patients

  • D. Wang
  • P. Clement
  • J. Smitz
  • M. -P. Derde
Original Paper


By using a microsuction technique, a quantitative determination of chemical mediators in nasal secretions was performed in 18 hay-fever patients and in a control group of 10 healthy volunteers. The authors then compared these quantitative data for mediators with objective nasal findings counting the number of sneezes, passive anterior rhinomanometry (PAR) and nasal inspiratory peak flow. A sampling protocol was designed with a follow-up of 3 days after nasal allergen challenge (NAC) in order to investigate both early and late allergic reactions. Median baseline concentrations of five major mediators were obtained: histamine, 19 ng/g; leukotriene C4 (LTC4), 5.7 ng/g. tryptase, 0; prostaglandin D2 (PGD2), 477 pg/g; eosinophil cationic protein (ECP), 105 ng/g. Significant increases in histamine (214 ng/g) , LTC4 (20 ng/g) and tryptase (28 μU/g) were found, but a significant decrease occurred in ECP (47 ng/g) and PGD (226 pg/g) immediately after NAC in the patients studied. Most ECP concentrations (94%) increased slowly 1 h after NAC and reached a significantly higher level 24 h later. In evaluating nasal symptoms, sneezes were present in a high percentage of cases (76%) during the early phase but were uncommon during the late phase (29%). Total nasal obstruction occurred in 94% during the early phase. In contrast, unilateral nasal obstruction presented in 82% during the late phase, whereas total nasal obstruction was present only in 41%. The most common type of late phase nasal obstruction shown by PAR was alternating nasal obstruction.

Key words

Nasal allergy Biochemical mediators Nasal symptoms Nasal secretions Microsuction 


  1. 1.
    Biewenga J, Stoop AE, Baker HE, Swart SJ, Nauta JJP, Van Kamp GJ, Vander Baan S (1991) Nasal secretions from patients with polyps and healthy individuals, collected with a new aspiration system: evaluation of total protein and immunoglobulin concentrations. Ann Clin Biochem 28:260–266PubMedGoogle Scholar
  2. 2.
    Bisgaard H, Grønborg H, Mygind N, Dahl R, Lindqvist N, Venge P (1990) Allergen-induced increase of eosinophil cationic protein in nasal lavage fluid: effect of the glucocorticoid budesonide. J Allergy Clin Immunol 85:891–8953PubMedCrossRefGoogle Scholar
  3. 3.
    Clement PAR, Van Dishoeck A, Van de Wal J, Stoop P, Hoek T, Van Strick R (1981) Nasal provocation and passive anterior rhinomanometry (PAR). Clin Allergy 11:293–301PubMedCrossRefGoogle Scholar
  4. 4.
    Gerth Van Wijk R, Dieges PH (1987) Comparison of nasal responsiveness to histamine, methacholine and phentolamine in allergic rhinitis patients and controls. Clin Allergy 17:563–570PubMedCrossRefGoogle Scholar
  5. 5.
    Juliusson S, Holmberg K, Baumgarten CR, Olsson M, Enander I, Pipkorn U (1991) Tryptase in nasal lavage fluid after local allergen challenge. Allergy 46:459–465PubMedGoogle Scholar
  6. 6.
    Naclerio RM, Meier HL, Kagey-Sobotka A, Adkinson Jr NF, Meyers DA, Norman PS, Lichtenstein LM (1983) Mediator release after nasal airway challenge with allergen. Am Rev Respir Dis 128:597–602PubMedGoogle Scholar
  7. 7.
    Naclerio RM, Proud D, Peters SP, Silber G, Kagey-Sobotka A, Adkinson NF Jr, Lichtenstein LM (1986) Inflammatory mediators in nasal secretions during induced rhinitis. Clin Allergy 16:101–110PubMedCrossRefGoogle Scholar
  8. 8.
    Nishioka K, Saito C, Nagano T, Okano M, Masuda Y, Kuriyama T (1993) Eosinophil cationic protein in the nasal secretions of patients with mite allergic rhinitis. Laryngoscope 103:189–192PubMedGoogle Scholar
  9. 9.
    Svensson C, Andersson M, Persson CGA, Venge P, Alkner U, Pipkorn U (1990) Albumin, bradykinins, and eosinophil cationic protein on the nasal mucosal surface in patients with hay fever during natural allergen exposure. J Allergy Clin Immunol 85: 828–833PubMedCrossRefGoogle Scholar
  10. 10.
    Wachs M, Proud D, Lichtenstein LM, Kagey-Sobotka A, Norman PS, Naclerio RM (1989) Observations on the pathogenesis of nasal priming. J Allergy Clin Immunol 84:492–501PubMedCrossRefGoogle Scholar
  11. 11.
    Zeiger RS (1989) Allergic and nonallergic rhinitis; classification and pathogenesis. 1. Allergic rhinitis. Am J Rhinol 3:21–47Google Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • D. Wang
    • 1
  • P. Clement
    • 1
  • J. Smitz
    • 2
  • M. -P. Derde
    • 3
  1. 1.Department of OtorhinolaryngologyUniversity Hospital, Free University of BrusselsBrusselsBelgium
  2. 2.Department of RadioimmunologyUniversity Hospital, Free University of BrusselsBrusselsBelgium
  3. 3.Department of Biomedical StatisticsFree University of BrusselsBrusselsBelgium

Personalised recommendations