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Platelets as participants in hyperacute guinea pig-to-rat lung xenorejection

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Abstract

It is well known that xenotransplantation leads to immediate graft dysfunction. This study was designed to specifically examine the role of platelets in mediating lung hyperacute xenorejection (HXR) in a guinea pig-to-rat model. A total of 18 lungs were perfused with blood using an ex vivo apparatus. The animals were divided into the following four groups: a CE group comprising circuit only with rat blood; a SYN group comprising rat lungs and blood; a XE group comprising guinea pig lungs and rat blood; and an SH group comprising guinea pig lungs and rat blood with sarpogrelate hydrochloride, a suppresser of platelet aggregation. The platelet and serotonin in the blood were lower and the wet/dry weight ratio of the lung (W/D) in the XE group were higher than those in the SYN group after perfusion. The platelet count was higher, but the serotonin and W/D were lower in the SH group than in the XE group. These results suggest that platelets strongly affect HXR. Thus, the administration of drugs to suppress platelet aggregation would reduce xenotransplanted lung edema.

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References

  1. Lin SS, Platt JL (1996) Immunologic barriers to xenotransplantation. J Heart Lung Transplant 15:547–555

    CAS  PubMed  Google Scholar 

  2. Egan TM, Cooper JD (1991) The lung following transplantation. In: Crystal RG, West JB, et al. (eds) The lung Raven, New York, pp 2205–2215

    Google Scholar 

  3. Kroshus TJ, Bolman RM III, Dalmasso AP (1996) Selective IgM depletion prolongs organ survival in an ex vivo model of pig-to-human xenotransplantation. Transplantation 62:5–12

    Article  CAS  PubMed  Google Scholar 

  4. Hara H, Osakabe M, Kitajima A, Tamao Y, Kikumoto R, (1991) MCT-9042, a new antiplatelet agent is a selective S2-serotonergic receptor antagonist. Thormb Haemost 65:415–420

    CAS  Google Scholar 

  5. Tsuchihashi H, Yagi N, Kimura M, Shirota K, Kitami J, Nagatomo T (1991) Binding characteristics of [3H]ketanserin for serotonin-2 receptor in the rabbit platelet. J Pharmacobiol Dyn 14:461–466

    CAS  Google Scholar 

  6. Iuliano L, Colavita AR, Leo R, Pratico D, Violi F (1997) Oxygen free radicals and platelet activation. Free Radic Biol Med 22:999–1006

    Article  CAS  PubMed  Google Scholar 

  7. Colman RB, Habal M, Hollenberg NK, Birtch AG, Busch GJ (1976) Hyperacute renal allograft rejection in the primate: therapeutic limitations of antiplatelet agents alone and combined with heparin. Am J Pathol 82:25–42

    CAS  PubMed  Google Scholar 

  8. Shons AR, Najarian JS (1974) Modification of xenograft rejection by aspirin, dextran, and cinanserin: the importance of platelets in hyperacute rejection. Transplant Proc 6:435–440

    CAS  PubMed  Google Scholar 

  9. Adachi H, Rosengard BR, Hutchins GM, Hall TS, Baumgartner WA, Borkon AM, Reitz BA (1987) Effects of cyclosporine, aspirin, and cobra venom factor on discordant cardiac xenograft survival in rats. Transplant Proc 19:1145–1148

    CAS  PubMed  Google Scholar 

  10. Ito S, Camussi G, Tetta C, Milgrom K, Andres G (1984) Hyperacute renal allograft rejection in the rabbit: the role of plateletactivating factor and of cationic proteins derived from polymorphonuclear leukocytes and from platelets. Lab Invest 51:148–161

    CAS  PubMed  Google Scholar 

  11. Cywes R, Mullen JBM, Stratis MA, Greig PD, Levy GA, Harvey PRC, Strasberg SM (1993) Prediction of the outcome of transplantation in man by platelet adherence in donor liver allografts: evidence of the importance of preservation injury. Transplantation 56:316–323

    Article  CAS  PubMed  Google Scholar 

  12. Bustos M, Platt JL (1997) Platelet-endothelial cell interactions in a xenograft model. Transplant Proc 29:886

    Article  CAS  PubMed  Google Scholar 

  13. Hattori R, Hamilton KK, Fugate RD, McEver RP, Sims PJ (1989) Stimulated secretion of endothelial von Willebrand factor is accompanied by rapid redistribution to the cell surface of the intracellular granule membrane protein GMP-140. J Biol Chem 264:7768–7771

    CAS  PubMed  Google Scholar 

  14. Zehr KJ, Herskowitz A, Lee PC, Kumar P, Gillinov AM, Baumgartner WA (1994) Neutrophil adhesion and complement inhibition prolongs survival of cardiac xenografts in discordant species. Transplantation 57:900–906

    Article  CAS  PubMed  Google Scholar 

  15. Frishman WH, Huberfeld S, Okin S, Wang YH, Kumar A, Shareef B (1995) Serotonin and serotonin antagonism in cardiovascular and noncardiovascular disease. J Clin Pharmacol 35:541–572

    CAS  PubMed  Google Scholar 

  16. Thomas DP, Vane JR (1967) 5-Hydroxytryptamine in the circulation of the dog. Nature 216:335–338

    Article  CAS  PubMed  Google Scholar 

  17. Herve P, Launay JM, Scrobohaci ML, Brenot F, Simonneau G, Petitpretz P, Poubeau P, Cerrina J, Duroux P, Drouet L (1995) Increased plasma serotonin in pulmonary hypertension. Am J Med 99:249–254

    Article  CAS  PubMed  Google Scholar 

  18. Yuan XJ, Bright RT, Aldinger AM, Rubin LJ (1997) Nitric oxide inhibits serotonin-induced calcium release in pulmonary artery smooth muscle cell. Am J Physiol 272 (Lung Cell Mol Physiol 16):L44-L50

    CAS  PubMed  Google Scholar 

  19. Sheldon WS, Crawford MP, Brewer NR (1979) Morphophysiology. In: Baker HJ, Lindsey JR, Weisbroth SH (eds) The laboratory rat. Academic, New York, pp 83–85

    Google Scholar 

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Authors and Affiliations

  1. First Department of Surgery, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, 142, Tokyo, Japan

    Makoto Nonaka, Mitsutaka Kadokura, Daisuke Kataoka, Shigeru Yamamoto, Noboru Tanio, Koichi Inoue & Toshihiro Takaba

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  1. Makoto Nonaka
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  2. Mitsutaka Kadokura
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  3. Daisuke Kataoka
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  4. Shigeru Yamamoto
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  5. Noboru Tanio
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  6. Koichi Inoue
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  7. Toshihiro Takaba
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Nonaka, M., Kadokura, M., Kataoka, D. et al. Platelets as participants in hyperacute guinea pig-to-rat lung xenorejection. Surg Today 29, 255–259 (1999). https://doi.org/10.1007/BF02483016

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  • DOI: https://doi.org/10.1007/BF02483016

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