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Blut

, Volume 47, Issue 2, pp 105–113 | Cite as

Analysis of isoenzyme patterns of acid phosphatase in acute leukemias

  • Hans Günter Drexler
  • Gerhard Gaedicke
Original Articles

Summary

The status of acid phosphatase isoenzymes was evaluated in cells of patients with acute lymphocytic leukemias or lymphomas by analytical isoelectric focusing in polyacrylamide gels (IEF) on horizontal thin-layer slabs. The isoenzyme patterns were correlated with routine immunological cell surface markers and the relationship of enzyme activity to specific immunological subclasses of ALL is discussed.

By isoelectric focusing up to five isoenzyme groups (I–V) containing several isoenzyme were observed. No leukemia specific or additional isoenzyme could be demonstrated. This biochemical characterization showed a marked heterogeneity within two major immunologic subgroups indicating that various differentiation stages of cell maturation could be involved in cALL and T-ALL. According to their degree of maturation along T-cell differentiation axis the leukemic cells displayed no enzyme activity, weak isoenzyme bands or the incomplete or complete isoenzyme pattern seen with normal lymphocytes from human tonsils which were used as controls. The investigation of specific enzymatic patterns can lead to a further definition of subsets of acute leukemias and give insight into lymphopoietic differentiation.

Key words

Childhood ALL subclassification Enzyme markers Acid phosphatase 

Abbreviations

AcP

acid phosphatase

ALL

acute lymphocytic leukemia

AML

acute myelocytic leukemia

AMML

acute myelomonocytic leukemia

AUL

acute undifferentiated leukemia

cALL

common ALL, ALL with the major or common phenotype (cALL-Ag positive)

cALL-Ag

common ALL-antigen

c/T-ALL

ALL subgroup with blast cells of an intermediate phenotype expressing both cALL-and T-antigens (cALL-Ag+T-Ag)

E

sheeperythrocyte rosette receptor

IEF

isoelectric focusing

PAGE

polyacrylamide gel electrophoresis

preT-ALL

form of ALL with lymphoblasts expressing only T-cell antigens (T-Ag) without an E-receptor (E)

SmIg

surface membrane immunoglobulin

T-Ag

T-cell antigen

T-ALL

Thymus derived acute lymphocytic leukemia

TEMED

tetramethyl-ethylenediamine

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Hans Günter Drexler
    • 1
  • Gerhard Gaedicke
    • 1
  1. 1.Abteilung Pädiatrie IIZentrum für Kinderheilkunde der Universität UlmUlm (Donau)Federal Republic of Germany

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