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Establishment and characterization of human pancreatic carcinoma lines with a high metastatic potential in the liver of nude mice

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Abstract

To investigate of human pancreatic cancer metastasis to the liver, a pancreatic carcinoma line, HPC-3, was injected into the spleens of nude mice. The cells from a few liver metastatic foci of the mice injected with HPC-3 were expanded in vitro and subsequently injected into the spleens of nude mice. By repeating these procedures, we were able to obtain a cell line, designated HPC-3H4. The mice were observed to have liver metastasis in 6 of 6 (100%) cases injected with HPC-3H4, whereas the rate was 0% at 3 weeks after the intrasplenic injection of HPC-3. The tumorigenicity of HPC-3H4 was more rapid than that of HPC-3. The motile activity of HPC-3H4 was also stronger than that of HPC-3, and the adhesion to the extracellular matrix of HPC-3H4 was stronger than that of HPC-3. We also analyzed the cell surface expression of the metastasis-related adhesion molecules. As a result, no substantial changes were observed in the expression level of adhesion molecules. These results suggest that HPC-3H4 is useful for studies aimed at the prevention of liver metastasis.

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Authors and Affiliations

  1. First Department of Surgery, Sapporo Medical University School of Medicine, South 1, West 16, Chuo-ku, 060-0061, Sapporo, Japan

    Takayuki Shishido, Takahiro Yasoshima, Koichi Hirata, Ryuichi Denno, Mitsuhiro Mukaiya, Hideki Ura & Koji Yamaguchi

  2. Department of Orthopedic Surgery, Sapporo Medical University School of Medicine, South 1, West 16, Chuo-ku, 060-0061, Sapporo, Japan

    Satoshi Kawaguchi

  3. First Department of Pathology, Sapporo Medical University School of Medicine, South 1, West 16, Chuo-ku, 060-0061, Sapporo, Japan

    Noriyuki Sato

Authors
  1. Takayuki Shishido
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  2. Takahiro Yasoshima
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  3. Koichi Hirata
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  4. Ryuichi Denno
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  5. Mitsuhiro Mukaiya
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  6. Hideki Ura
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  7. Koji Yamaguchi
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  8. Satoshi Kawaguchi
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  9. Noriyuki Sato
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Shishido, T., Yasoshima, T., Hirata, K. et al. Establishment and characterization of human pancreatic carcinoma lines with a high metastatic potential in the liver of nude mice. Surg Today 29, 519–525 (1999). https://doi.org/10.1007/BF02482346

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  • DOI: https://doi.org/10.1007/BF02482346

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