Abstract
The usefulness of phosphorus-31 nuclear magnetic resonance spectroscopy (31P NMRS) from detecting heart graft rejection after transplantation has been investigated by several researchers, and it has thus been demonstrated to be a valid technique for detecting acute myocardial rejection. In this study, we investigated the value of31P NMRS to assess chronic cardiac allograft rejection. Lewis rat hearts were transplanted into the femoral region of F-344 rat recipients which were treated with cyclosporine, 5 mg/kg body weight, by a daily intramuscular injection for 30 days beginning on the day of transplantation. The control isografts employed Lewis donors and recipients not given cyclosporine. The ratios of phosphocreatine (PCr) to inorganic phosphate (Pi), β-adenosine trisphosphate (β-ATP) to Pi, and PCr to β-ATP were monitored using surface coil31P NMRS.31P NMRS was performed 3, 30, and 60 days after transplantation, and the degree of the rejection and arteriosclerosis of the coronary arteries were then assessed histologically. The PCr:Pi and β-ATP:Pi ratios for the allografts demonstrated a significant decrease on postoperative day (POD) 60 from that on POD 30 (PCr:Pi,P<0.001; β-ATP:Pi,P<0.01). Although a significant difference existed between the isografts and allografts on POD 60 (PCr:Pi,P<0.01; β-ATP:Pi,P<0.01), no significant difference was found in the PCr:β-ATP ratio between the allografts and the isografts. On POD 60, the allografts showed significant graft rejection and arterio-sclerotic changes in the coronary arteries. These findings therefore demonstrated the effectiveness of31P NMRS for detecting chronic graft rejection in a rat model.
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Suzuki, K., Hamano, K., Ito, H. et al. The detection of chronic heart graft rejection by31P NMR spectroscopy. Surg Today 29, 143–148 (1999). https://doi.org/10.1007/BF02482239
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DOI: https://doi.org/10.1007/BF02482239