Summary
The hemodynamic and metabolic effects of diaspirin crosslinked hemoglobin (DCLHb) were investigated using graded treadmill exercise in swine (n = 5/group). Swine received DCLHb (10% solution, 5 ml/kg) or oncotically-matched human serum albumin (HSA, 5 ml/kg). Baseline metabolic and hemodynamic data were similar. In both groups exercise increased hemodynamic parameters. Exercise increased heart rate (HR) from 139 ± 12 to 293 ± 28 bpm with DCLHb and from 136 ± 13 to 314 ± 13 bpm with HSA. Exercise increased cardiac output (CO) from 5.7 ± 0.75 to 15.6 ± 2.01/min in the DCLHb group and from 5.3 ± 0.48 to 15.7 ± 0.881/min in the HSA group. However, CO returned to baseline faster with DCLHb upon stopping exercise. The DCLHb-treated group demonstrated a significantly higher oxygen extraction during exercise (12.04 ± 0.38 vs 9.48 ± 0.99 ml O2/100 ml blood) and a lower oxygen delivery throughout recovery (74.6 ± 6.6 vs 102.2 ± 7.21 O2/min), indicating enhanced oxygen delivery during exercise in the treatment group. DCLHb infusion did not impair metabolic or hemodynamic functions. These data indicate that DCLHb may increase oxygen delivery to working tissue more efficiently than HSA during treadmill exercise in swine.
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Mautz WJ, Phalen RF, McClure TR, Bufalino C (1985) A rodent treadmill for inhalational toxicology studies and respirometry. J Appl Physiol 58:673–679
Sumitomo N, Ito S, Harada K, Kobayashi H, Okuni M (1986) Treadmill exercise test in children with cardiomyopathy and postmyocarditic myocardial hypertrophy. Heart Vessels 2:47–50
Leger L, Mercier D (1984) Gross energy cost of horizontal treadmill and track running. Sports Med 1:270–277
Marchand G, Dunlap E, Farrell L, Nigro C, Burhop K (1996) Resuscitation with increasing doses of diasiprin crosslinked hemoglobin in swine. Artif Cells Blood Substit Immobil Biotechnol 24:469–487
Powell CC, Schultz SC, Malcolm DS (1996) Diaspirin crosslinked hemoglobin (DCLHb): more effective than lactated Ringer's solution in restoring central venous oxygen saturation after hemorrhagic shock in rats. Artif Cells Blood Substit Immobil Biotechnol 24:197–200
Schultz SC, Powell CC, Burris DG, Nguyen H, Jaffin J, Malcolm DS (1994) The efficacy of diaspirin crosslinked hemoglobin solution resuscitation in a model of uncontrolled hemorrhage. J Trauma 37:408–412
Nelson DJ (1998) HemAssistTM: development and clinical profile. In: Rudolph AS, Rabinovici R, Feuerstein GZ (eds) Red blood cell substitutes: basic principles and clinical applications. Marcel Dekker, New York, pp 353–400
Nelson D, Hai T, Srnak A, Ebling A, Kunas G, Catarello J, Burhop K (1992) Synthesis and properties of polymerized, diaspirin cross-linked hemoglobins. Biomater Artif Cells Immobil Biotechnol 20:253–258
Barve A, Sen AP, Saxena PR, Gulati A (1997) Dose response effect of diaspirin crosslinked hemoglobin (DCLHb) on systemic hemodynamics and regional blood circulation in rats. Artif Cells Blood Substit Immobil Biotechnol 25:75–84
Burhop KE, Farrell L, Nigro C, Tan D, Estep T (1992) Effects of intravenous infusions of diaspirin crosslinked hemoglobin (DCLHb) on sheep. Biomater Artif Cells Immobil Biotechnol 20:581–585
Sharma AC, Gulati A (1994) Effects of diaspirin crosslinked hemoglobin and norepinephrine on systemic hemodynamics and regional circulation in rats. J Lab Clin Med 123:299–308
Sharma AC, Singh G, Gulati A (1995) Role of NO mechanisms in cardiovascular effects of diaspirin crosslinked hemoglobin in anesthetized rats. Am J Physiol 269:H1379-H1388
Frankel HL, Nguyen HB, Shea-Donohue T, Aiton LA, Ratigan J, Malcolm DS (1996) Diaspirin crosslinked hemoglobin is efficacious in gut resuscitation as measured by a GI tract optode. J Trauma 40:231–240
Freas W, Llave R, Jing M, Hart J, McQuillan P, Muldoon S (1995) Contractile effects of diaspirin crosslinked hemoglobin (DCLHb) on isolated porcine vessels. J Lab Clin Med 125:762–767
Kolkhorst FW, Mittelstadt SW, Dolgener FA (1996) Perceived exertion and blood lactate concentration during graded treadmill running. Eur J Appl Physiol 72:272–277
Noakes TD (1997) Challenging beliefs: ex Africa semper aliquid novi. Med Sci Sports Exerc 25:571–590
Wasserman K, Beaver WL, Whipp BJ (1990) Gas exchange theory and the lactic acidosis (anaerobic) threshold. Circulation 81:14–30
Vandergriff KD, Medina F, Marini MA, Winslow RM (1989) Equilibrium oxygen binding to human hemoglobin cross-linked between the α chains by bis(3,5-dibromosalicyl)fumarate. J Biol Chem 264:17824–17833
Bush S, Marshall T, Spicuzza J, Nelson D (1994) Diaspirin crosslinked hemoglobin (DCLHb): biolytical studies in swine. Artif Cells Blood Substit Immobil Biotechnol 22:917–922
McKenzie JE, Cost EA, Scandling DM, Ahle NW (1994) Effects of diaspirin crosslinked hemoglobin during coronary angioplasty in the swine. Cardiovasc Res 28:1188–1192
Sharma AC, Rebello S, Gulati A (1994) Regional circulatory and systemic hemodynamic effects of diaspirin crosslinked hemoglobin in rats. Artif Cells Blood Substit Immobil Biotechnol 22:593–602
Gulati A, Rebello S (1994) Role of adrenergic mechanisms in the pressor effect of diaspirin crosslinked hemoglobin. J Lab Clin Med 124:125–133
Lloyd-Jones DM, Bloch KD (1996) The vascular biology of nitric oxide and its role in atherogenesis. Annu Rev Med 47:365–375
Nava E, Noll G, Luscher TF (1995) Nitric oxide in cardiovascular diseases. Ann Med 27:343–351
Cole DJ, Drummond JC, Patel PM, Nary JC, Reynolds L (1996) Effect of oncotic pressure of diaspirin crosslinked hemoglobin (DCLHb) on brain injury after temporary focal cerebral ischemic in rats. Anesth Analg 83:342–347
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Crago, M.S., West, S.D. & McKenzie, J.E. Effects of diaspirin crosslinked hemoglobin infusion in treadmill-exercised swine. Heart Vessels 14, 1–8 (1999). https://doi.org/10.1007/BF02481737
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DOI: https://doi.org/10.1007/BF02481737