Skip to main content
SpringerLink
Log in

Kinetic study of glomerular epithelial cells associated with segmental glomerular sclerotic lesions with adhesion in spontaneously diabetic WBN/Kob rats

  • Original Article
  • Experimental Investigation
  • Published:
Clinical and Experimental Nephrology Aims and scope Submit manuscript

Abstract

Background

We previously found that glomerular epithelial cells play an important role in the formation of adhesive lesions. Glomerular sclerotic lesions develop after the inital adhesive lesions.

Methods

Two series of experiments were done with spontaneously diabetic WBN/Kob rats. These rats develop segmental glomerular sclerotic lesions with aging. The first series of experiments was intended to clarify the kinetics of glomerular cells on progressive glomerular damage in these rats. The second series of experiments was designed to study the relationship between proliferation (judged by % bromodeoxyuridine-positive cells) of glomerlar epithelial cells and sclerotic lesions with adhesions.

Results

In the first series, rats having increased proteinuria showed segmental glomerular sclerotic lesions with adhesions. At the same time, increased labeling indices of tuft cells and epithelial cells of Bowman's capsule were observed. In the second series, no significant increase in the labeling indices of tuft cells with sclerotic lesions was observed, compared to tuft cells without sclerotic lesions. In sclerotic lesions with adhesion, bromodeoxyurdine-positive cells were observed that were not distinguishable as podocytes or epithelial cells of Bowman's capsule. The highest labelling index was noted in the epithelial cells of Bowman's capsules with sclerosis.

Conclusion

This study shows that the proliferation of glomerular epithelial cells (mainly epithelial cells of Bowman's capsule) occurs in glomerular sclerotic lesions with adhesions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Lovett DH, and Sterzel RB. Cell culture approaches to the analysis of glomerular inflammation. Kidney Int 1986;30:246–254.

    PubMed  CAS  Google Scholar 

  2. Striker LJ, Peten EP, Elliot SJ, Doi T, Striker GE. Mesangial cell turnover: effect of heparin and peptide growth factors. Lab Invest 1991;64:446–456.

    PubMed  CAS  Google Scholar 

  3. Mene PM, Simonson MS, Dunn MJ. Physiology of the mesangial cell. Physiol Rev 1989;69:1374–1424.

    Google Scholar 

  4. Abboud HE. Nephrology forum: growth factors in glomerulonephritis. Kidney Int 1993;43:252–267.

    PubMed  CAS  Google Scholar 

  5. Olson JL, Heptinstall RH. Biology of disease. Nonimmunologic mechanisms of glomerular injury. Lab Invest 1988;59:564–578.

    PubMed  CAS  Google Scholar 

  6. Osawa G, Sasaki T, Sato T, Tamai H, Nomura S, Ishimatsu T. Role of glomerular epithelial cells in progression of renal disease. In Chugh KS (ed) Asian nephrology. London: Oxford University Press, 1994:270–278.

    Google Scholar 

  7. Kihara I, Yaoita E, Kawasaki K, Yamamoto T. Cellular process of glomerular adhesion in aged rats. Acta Med Biol 1990;34(suppl 2):S69-S80.

    Google Scholar 

  8. Kondo Y, Akikusa C. Chronic Masugi nephritis in the rat. An electron microscopic study on evolution and consequences of glomerular capsular adhesions. Acta Pathol Jpn 1982;32:231–242.

    PubMed  CAS  Google Scholar 

  9. Nagata M, Kriz W. Glomerular damage after uninephrectomy in young rats. II Mechanical stress on podocytes as a pathway to sclerosis. Kidney Int 1992;42:148–160.

    PubMed  CAS  Google Scholar 

  10. Nakama K, Shichinohe K, Kobayashi K Naito K, Uchida O, Yasuhara K, Tobe M. Spontaneous diabeticlike syndrome in WBN/Kob rats. Acta Diabetol Lat 1985;22:335–342.

    PubMed  CAS  Google Scholar 

  11. Tsuchitani M, Saegusa T, Narama I, Nishikawa T, Gonda Y. A new diabetic strain of rat (WBN/Kob) Lab Anim 1989;60:205–218.

    Google Scholar 

  12. Sato T. Ultrastructural study of glomerular epithelial cells in a strain of spontaneously diabetic WBN/Kob rats. J Kawasaki Med Soc (Kawasaki Igakkai Shi) 1992;18:289–304 (in Japanese)

    Google Scholar 

  13. Grantzer HG. Monoclonal antibody to 5-bromodexyuridine: a new reagent for detection of DNA replication. Science 1982;218:474–475.

    Google Scholar 

  14. Frankel S, Reitman S, Sonnenwirth AC. Gradwohl's Clinical Laboratory Methods and Diagnosis. St Louis: Mosby, 1970.

    Google Scholar 

  15. Sasaki T, Osawa G. A kinetic study of the glomerular cells of developing and mature rat kidneys using an anti-bromodeoxyuridine monoclonal antibody. Jpn J Nephrol 1993;35:1213–1219.

    CAS  Google Scholar 

  16. Howie AJ, Kizaki T, Beaman M, Morland CM, Birtwistle RJ, Adu D, Michael J, Williams AJ, Walls J, Matsuyama M, Shimizu F. Different types of segmental sclerosing glomerular lesions in six experimental models of proteinuria. J Pathol 1989;157:141–151.

    Article  PubMed  CAS  Google Scholar 

  17. Eisen HN. Adenomatoid transformation of the glomerular capsular epithelium. Am J Pathol 1946;22:597–601.

    Google Scholar 

  18. Reidbord HE. Metaplasia of the parietal layer of Bowman's capsule. Am J Clin Pathol 1968;50:240–242.

    PubMed  CAS  Google Scholar 

  19. Hughson MD, McManus JFA, Hennigar GR. Studies on “end-stage” kidneys. Embryonal hyperplasia of Bowman's capsular epithelium. Am J Pathol 1978;91:71–84.

    PubMed  CAS  Google Scholar 

  20. Marcus PB. Podocytic “metaplasia” of parietal Bowman's capsular epithelium. Arch Pathol Lab Med 1981;29:209–215.

    Google Scholar 

  21. Gaffney EF, Panner BJ. Membranous glomerulonephritis: clinical significance of glomerular hypercellularity and parietal epithelial abnormalities. Nephron 1981;29:209–215.

    Article  PubMed  CAS  Google Scholar 

  22. Sasaki T, Jyo Y, Tanda N, Tamai H, Osawa G. The role of basic fibroblast grwoth factor (FGF2) in glomerular epithelial cell injury. In: Koide H, Ichikawa I (ed) Progression of renal diseases. Contrib Nephrol 118 Basel: Karger, 1996:68–77.

    Google Scholar 

  23. Pabst R, Sterzel RB. Cell renewal of glomerular cell types in normal rats: an autoradiographic analysis. Kidney Int 1983;24:626–631.

    PubMed  CAS  Google Scholar 

  24. Rasch R, Nogaard JOR. Renal enlargement: comparative autoradiographic studies of [3H]-thymidine uptake in diabetic and uninephrectomized rats. Diabetologia 1983;25:280–287.

    Article  PubMed  CAS  Google Scholar 

  25. Fries JW, Sandstrom DJ, Meyer TW, Rennke HG. Glomerular hypertrophy and epithelial cell injury modulate progressive glomerulosclerosis in the rat. Lab Invest 1989;60:205–218.

    PubMed  CAS  Google Scholar 

  26. Sterzel PB, Pabst R, Kregeler M, Perfetto M. The temporal relationship between glomerular cell proliferation and monocyte infiltration in experimental glomerulonephritis. Virchows Arch B Cell Pathol 1982;38:337–350.

    CAS  Google Scholar 

  27. Kihara I, Yaoita E, Kawasaki K, Yamamoto T. Limitation of podocyte adaptation for glomerular injury in puromycin aminonucleoside nephrosis. Pathol Int 1995;45:625–634.

    PubMed  CAS  Google Scholar 

  28. Yamazaki T. Podocytic degeneration and regeneration in puromycin aminonucleoside nephropathy in the rat. Pathol Int 1995;45:465–475.

    Article  PubMed  CAS  Google Scholar 

  29. Kriz W, Hahnel B, Rosener S, Elger M. Long-term treatment of rats with FGF-2 results in focal segmental glomerulosclerosis. Kidney Int 1995;48:1435–1450.

    PubMed  CAS  Google Scholar 

  30. Polunovsky VA, Chen B, Henke C, Snover D, Wendt C, Ingbar DH, Bitterman PB. Role of mesenchymal cell death in lung remodeling after injury. J Clin Invest 1992;92:388–397.

    Article  Google Scholar 

  31. Shimizu A, Yamanaka N. Apoptosis and cell desquamation in repair process in ischemic tubular necrosis. Virchow Archive B Cell Pathol 1993;64:171–180.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Nephronlogy, Department of Internal Medicine, Kawasaki Medical School, 577 Matsushima, 701-01, Kurashiki, Japan

    Tamaki Sasaki, Tetsuya Sato, Yoshiyuki Jyo, Nobuya Tanda, Hitoshi Tamai & Gengo Osawa

Authors
  1. Tamaki Sasaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tetsuya Sato
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yoshiyuki Jyo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Nobuya Tanda
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hitoshi Tamai
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Gengo Osawa
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Sasaki, T., Sato, T., Jyo, Y. et al. Kinetic study of glomerular epithelial cells associated with segmental glomerular sclerotic lesions with adhesion in spontaneously diabetic WBN/Kob rats. Clin Exper Neph 1, 32–40 (1997). https://doi.org/10.1007/BF02480653

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02480653

Key words

Search

Navigation