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Development of a hybrid artificial liver support system and preclinical animal experiments

  • Tissue Engineering: Review
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References

  1. Schraa EO, Marquet RL, IJzermans JNM. The fourth barrier. Curr Med Res Opin 1999;15:327–338

    Article  PubMed  CAS  Google Scholar 

  2. Sim KH, Marinov A, Levy GA. Xenotransplantation: a potential solution to the critical organ donor shortage. Can J Gastroenterol 1999;13:311–318

    PubMed  CAS  Google Scholar 

  3. Falkenhagen D, Strobl W, Vogt G, Schrefl A, Linsberger I, Gerner FJ, Schoenhofen M. Fractionated plasma separation and adsorption system: a novel system for blood purification to remove albumin bound substances. Artif Organs 1999;23:81–86

    Article  PubMed  CAS  Google Scholar 

  4. Stange J, Mitzner SR, Risler T, Erley CM, Lauchart W, Goehl H, Klammt S, Peszynski P, Freytag J, Hickstein H, Lohr M, Liebe S, Schareck W, Hopt UT, Schmidt R. Molecular adsorbent recycling system (MARS): clinical results of a new membrane-based blood purification system for bioartificial liver support. Artif Organs 1999;23:319–330

    Article  PubMed  CAS  Google Scholar 

  5. Ellis AJ, Hughes RD, Nicholl D, Langley PG, Wendon JA, O'Grady JG, Williams R. Temporary extracorporeal liver support for severe acute alcoholic hepatitis using the BioLogic-DT. Int J Artif Organs 1999;22:27–34

    PubMed  Google Scholar 

  6. Steczko J, Ash SR, Blake DE, Carr DJ, Bosley RH. Cytokines and endotoxin removal by sorbents and its application in push-pull sorbent-based pheresis: the biologic-DTPF system. Artif Organs 1999;23:310–318

    Article  PubMed  CAS  Google Scholar 

  7. Enosawa S, Suzuki S, Kakefuda T, Amemiya H. Examination of 7-ethoxycoumarin deethylation and ammonia removal activities in 31 hepatocyte cell lines. Cell Transplant 1996;5:S39-S40

    Article  PubMed  CAS  Google Scholar 

  8. Takagi M, Fukuda N, Yoshida T. Comparison of different hepatocyte cell lines for use in a hybrid artificial liver model. Cytotechnology 1997;24:39–45

    Article  Google Scholar 

  9. Omasa T, Urayama S, Yamanaka M, Tanimura N, Katakura Y, Kishimoto M, Suga K, Enosawa S, Amemiya H. Construction of ammonia metabolizing cell line for artificial hybrid liver support system using recombinant DNA techniques. Kagaku Kogaku Ronbunshu 1998;24:184–189

    CAS  Google Scholar 

  10. Liu J, Pan J, Naik S, Santangini H, Trenkler D, Thompson N, Rifai A, Chowdhury JR, Jauregui HO. Characterization and evaluation of detoxification functions of a nontumorigenic immortalized porcine hepatocyte cell line (HepLiu). Cell Transplant 1999;8:219–232

    PubMed  CAS  Google Scholar 

  11. Sussman NL, Gislason GT, Conlin CA, Kelly JH. The hepatic extracorporeal liver assist device: initial clinical experience. Artif Organs 1994;18:390–396

    PubMed  CAS  Google Scholar 

  12. Ijima H, Nakazawa K, Funatsu K. Development of a hybrid artificial liver. Organ Biol 1998;5:35–40

    CAS  Google Scholar 

  13. Hasegawa H, Shimada M, Gion T, Ijima H, Nakazawa K, Funatsu K, Sugimachi K. Modulation of immunologic reactions between cultured porcine hepatocytes and human sera. ASAIO J 1999;45: 392–396

    PubMed  CAS  Google Scholar 

  14. Watanabe FD, Mullon C J-P, Hewitt WR, Arkadopoulos N, Kahaku E, Eguchi S, Khalili T, Arnaout W, Shackleton CR, Rozga J, Solomon B, Demetriou AA. Clinical experience with a bioartificial liver in the treatment of severe liver failure: a phase I clinical trial. Ann Surg 1997;225:484–494

    Article  PubMed  CAS  Google Scholar 

  15. Kardassis D, Busse B, Kraemer MR, Smith MD, Neuhaus P, Gerlach JC. Hemodynamic effects of therapy with a hybrid liver support system. ASAIO J 1999;45:203

    Google Scholar 

  16. Patzer JF II, Mazariegos GV, Kramer DJ, Lopez R, Giraldo M, de Vera M, McCormick E, Grogan TA, Zhu Y, Fulmer ML, Amiot BP, Starzl TE. Novel bioartificial liver support system (BLSS): clinical experience. Int J Artif Organs 1999;22:407

    Google Scholar 

  17. Nakazawa K, Matsushita T, Funatsu K. Prolonged lidocaine metabolizing activity of primary hepatocytes with spheroid culture using polyrethane foam as a culture substratum. Cytotechnology 1997;24:235–242

    Article  CAS  Google Scholar 

  18. Logan JS, Sharma A. Potential use of genetically modified pigs as organ donors for transplantation into humans. Clin Exp Pharmacol Physiol 1999;26:1020–1025

    Article  PubMed  CAS  Google Scholar 

  19. Gerlach JC, Brombacher J, Courtney JM, Neuhaus P. Nonenzymatic versus enzymatic hepatocyte isolation from pig livers for larger scale investigations of liver cells perfusion systems. Int J Artif Organs 1993;16:677–681

    PubMed  CAS  Google Scholar 

  20. Nakamura M, Koide N, Shiraha H, Ujike K, Shinji T, Hada H, Tsuji T. A large scale isolation of pig hepatocytes for a bioartificial liver support system. Jpn J Artif Organs 1996;25:674–677

    Google Scholar 

  21. Kasai S, Sawa M, Mito M. Hybrid artificial liver. Jpn J Artif Organs 1990;19:1545–1551

    Google Scholar 

  22. Koide N, Shinji T, Tanabe T, Asano K, Kawaguchi M, Sakaguchi K, Koide Y, Mori M, Tsuji T. Continued high albumin production by multicellular spheroids of adult rat hepatocytes formed in the presence of liver-derived proteoglycans. Biochem Biophys Res Commun 1989;161:385–391

    Article  PubMed  CAS  Google Scholar 

  23. Matsushita T, Ijima H, Koide N, Funatsu K. High albumin production by multicellular spheroids of adult rat hepatocytes formed in the pores of polyurethane foam. Appl Microbiol Biotechnol 1991;36:324–326

    Article  PubMed  CAS  Google Scholar 

  24. Langer R, Vacanti JP. Tissue engineering. Science 1993;260:920–926

    PubMed  CAS  Google Scholar 

  25. Kaihara S, Vacanti JP. Tissue engineering—toward new solutions for transplantation and reconstructive surgery. Arch Surg 1999;134:1184–1188

    Article  PubMed  CAS  Google Scholar 

  26. Peter SJ, Miller MJ, Yasko AW, Yaszemski MJ, Mikos AG. Polymer concepts in tissue engineering. J Biomed Mater Res 1998;43:422–427

    Article  PubMed  CAS  Google Scholar 

  27. Cusick RA, Lee HM, Sano KR, Pollok JM, Utsunomiya H, Ma PX, Langer R, Vacanti JP. The effect of donor and recipient age on engraftment of tissue-engineered liver. J Pediat Surg 1997;32:357–360

    Article  PubMed  CAS  Google Scholar 

  28. Marx U, Yalcin BE. European research and commercialization activities in the field of tissue engineering and liver support in world wide competition. Int J Artif Organs 1998;21:119–126

    PubMed  CAS  Google Scholar 

  29. Wu FJ, Friend JR, Remmel RP, Cerra FB, Hu WS. Enhanced cytochrome P450IAI activity of self-assembled rat hepatocyte spheroids. Cell Transplant 1999;8:233–246

    PubMed  CAS  Google Scholar 

  30. Ijima H, Nakazawa K, Mizumoto H, Matsushita T, Funatsu K. Formation of a spherical multicellular aggregate (spheroid) of animal cells in the pores of polyurethane foam as a cell culture substratum and its application to a hybrid artificial liver. J Biomater Sci Polymer Edn 1998;9:765–778

    CAS  Google Scholar 

  31. Ijima H, Matsushita T, Nakazawa K, Fujii Y, Funatsu K. Hepatocyte spheroids in polyurethane foams: functional analysis and application for a hybrid artificial liver. Tissue Eng 1998;4:213–226

    CAS  Google Scholar 

  32. Nakazawa K, Mizumoto H, Kaneko M, Ijima H, Gion T, Shimada M, Shirabe K, Takenaka K, Sugimachi K, Funatsu K. Formation of porcine hepatocyte spherical multicellular aggregates (spheroid) and analysis of drug metabolic functions. Cytotechnology 1999;31: 61–68

    Article  CAS  PubMed  Google Scholar 

  33. Ijima H, Nakazawa K, Kaneko M, Mizumoto H, Matsushita T, Gion T, Shimada M, Shirabe K, Takenaka K, Sugimachi K, Funatsu K. The formation of a spheroid of primary hepatocytes and the expression of liver-specific functions depend on the characteristics of polyurethane foam. J Artif Organs 1998;1:83–88

    Article  Google Scholar 

  34. Nakazawa K, Ijima H, Kaneko M, Ito T, Matsushita T, Gion T, Shirabe K, Shimada M, Takenaka K, Sugimachi K, Funatsu K. Investigation of optimum culture condition for hybrid artificial liver module using PUF/porcine hepatocyte spheroid culture. Jpn J Artif Organs 1998;27:222–226

    Google Scholar 

  35. Sakai Y, Suzuki M. Stable immobilization of hepatocyte spheroids rapidly formed by large-scale suspension culture. Artif Organs Today 1994;4:129–140

    Google Scholar 

  36. Ijima H, Matsushita T, Funatsu K. Development of a hybrid artificial liver using multi capillary PUF/spheroid packed bed. Jpn J Artif Organs 1994;23:463–468

    Google Scholar 

  37. Uchino J, Tsuburaya T, Kumagai F, Hase T, Hamada T, Komai T, Funatsu A, Hashimura E, Nakamura K, Kon T. A hybrid bioartificial liver composed of multiplated hepatocyte monolayers. ASAIO Trans 1988;34:972–977

    PubMed  CAS  Google Scholar 

  38. Rozga J, Williams F, Ro MS, Neuzil DF, Giorgio TD, Backfisch G, Moscioni AD, Hakim R, Demetriou AA. Development of a bioartificial liver: properties and function of a hollow-fiber module inoculated with liver cells. Hepatology 1993;17:258–265

    Article  PubMed  CAS  Google Scholar 

  39. Rozga J, Podesta L, LePage E, Morsiani F, Moscioni AD, Hoffman A, Sher L, Villamil F, Woolf G, McGrath M, Kong L, Rosen H, Lanman T, Vierling J, Makowka L, Demetriou AA. A bioartificial liver to treat severe acute liver failure. Ann Surg 1994;219:538–546

    PubMed  CAS  Google Scholar 

  40. Naka S, Takeshita K, Yamamoto T, Tani T, Kodama M. Bioartificial liver support system using porcine hepatocytes entrapped in a three-dimensional hollow fiber module with collagen gel: an evaluation in the swine acute liver failure model. Artif Organs 1999;23:822–828

    Article  PubMed  CAS  Google Scholar 

  41. Matsushita T, Ijima H, Wada S, Funatsu K. Estimation of the performance of a PUF/spheroid packed-bed type artificial liver by using an extracorporeal circulation with hepatic failure rats. Jpn J Artif Organs 1995;24:815–820

    Google Scholar 

  42. Ijima H, Matsushita T, Nakazawa K, Koyama S, Gion T, Shirabe K, Shimada M, Takenaka K, Sugimachi K, Funatsu K. Spheroid formation of primary dog hepatocytes using polyurethane foam and its application to hybrid artificial liver. In: Carrondo MJT, Griffiths B, Moreira JLP (eds) Animal cell technology. Dordrecht, Netherlands: Kluwer Academic Publishers, 1997;577–583

    Google Scholar 

  43. Gion T, Shimada M, Shirabe K, Nakazawa K, Ijima H, Matsushita T, Funatsu K, Sugimachi K. Evaluation of a hybrid artificial liver using a polyurethane foam packed-bed culture system in dogs. J Surg Res 1999;82:131–136

    Article  PubMed  CAS  Google Scholar 

  44. Kaneko M, Fukuda J, Ijima H, Nakazawa K, Gion T, Shimada M, Shirabe K, Takenaka K, Sugimachi K, Fumatsu K. Development of hybrid artificial liver support system using spheroid culture and application to warm ischemic liver failure in dog and pig as a preclinical test. Mater Sci Eng C6 1998;245–248

    Article  Google Scholar 

  45. Ijima H, Nakazawa K, Kaneko M, Katsuno T, Fukuda J, Shimada M, Utsunomiya T, Gion T, Yamashita Y, Sugimachi K, Funatu K. Preclinical animal experiment of a hybrid artificial liver. ASAIO J 1999;45:201

    Article  Google Scholar 

  46. Patience C, Takeuchi Y, Weiss RA. Infection of human cells by an endogenous retrovirus of pigs. Nature Medicine 1997;3:282–286

    Article  PubMed  CAS  Google Scholar 

  47. Birmingham K. FDA subcommittee finds no evidence of PERV transmission. Nature Med 1999;5:855

    PubMed  CAS  Google Scholar 

  48. Paradis K, Langford G, Long ZF, Heneine W, Sandstrom P, Switzer WM, Chapman LE, Lockey C, Onions D, Otto E. Search for cross-species transmission of porcine endogenous retrovirus in patients treated with living pig tissue. Science 1999;285:1236–1241

    Article  PubMed  CAS  Google Scholar 

  49. Pitkin Z, Mullon C. Evidence of absence of porcine endogenous retrovirus (PERV) infection in patients treated with a bioartificial liver support system. Artif Organ 1999;23:829–833

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan

    Hiroyuki Ijima DEng, Kohji Nakazawa DEng, Mitsuru Kaneko DS, Junji Fukuda DS & Kazumori Fumatsu DEng

  2. Department of Surgery II, Faculty of Medicine, Kyushu University, Fukuoka, Japan

    Mitsuo Shimada MD, Yo-ichi Yamashita MD, Tomonobu Gion MD, Ken Shirabe MD & Keizo Sugimachi MD

Authors
  1. Hiroyuki Ijima DEng
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  2. Kohji Nakazawa DEng
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  3. Mitsuru Kaneko DS
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  4. Junji Fukuda DS
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  5. Mitsuo Shimada MD
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  6. Yo-ichi Yamashita MD
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  7. Tomonobu Gion MD
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  8. Ken Shirabe MD
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  9. Keizo Sugimachi MD
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  10. Kazumori Fumatsu DEng
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Corresponding author

Correspondence to Kazumori Fumatsu DEng.

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Ijima, H., Nakazawa, K., Kaneko, M. et al. Development of a hybrid artificial liver support system and preclinical animal experiments. J Artif Organs 3, 112–116 (2000). https://doi.org/10.1007/BF02479976

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  • DOI: https://doi.org/10.1007/BF02479976

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