Role of secreted protein acidic and rich in cysteine (SPARC) in patients with diabetic nephropathy
- 40 Downloads
A protein called “secreted protein acidic and rich in cysteine” (SPARC) may be important in the progression of diabetic nephropathy. Recent animal studies have shown a possible correlation between SPARC levels and diabetes-related kidney growth.
We measured serum levels of SPARC in patients with diabetic nephropathy and compared them to the severity of glomerular lesions as determined by renal biopsy. A total of 50 non-insulin-dependent diabetic patients were divided into 2 groups; patients with endstage diabetic nephropathy (ESDN, n=10) or without ESDN (DM, n=40). Renal biopsies were performed in all patients without ESDN. Serum levels of SPARC were measured by enzyme-linked immunosorbent assay (ELISA) in the 50 patients with diabetes, in 10 healthy controls, and in 10 patients with immunoglobulin (Ig) A nephropathy. Values for serum creatinine, creatinine clearance, β2-microglobulin, and hemoglobin A1C also were obtained.
The mean serum SPARC levels (ng/mL) were 29±12, 41±21, 43±20, and 85±28, for healthy controls, IgA nephropathy, DM, and ESDN patients, respectively. Serum SPARC levels showed a significant increase with an increase in severity of glomerular diffuse lesions. Serum levels of SPARC were significantly higher in the DM group than in controls, and significantly higher in the ESDN group than in the control, IgA nephropathy and DM groups.
The findings suggest that SPARC may contribute to the progression of diabetic nephropathy.
Key wordsdiabetic nephropathy renal biopsy secreted protein acidic and rich in cysteine (SPARC)
Unable to display preview. Download preview PDF.
- 4.Pichler RH, Bassuk JA, Hugo C, Reed MJ, Eng E, Gordon KL, Pippin J, Alpers CE, Couser WG, Sage EH, Johnson RJ. SPARC is expressed by mesangial cells in experimental mesangial proliferative nephritis and inhibits platelet-derived growth factor mediated mesangial cell proliferation in vitro. Am J Pathol 1996;148:1153–1167.PubMedGoogle Scholar
- 8.Gellman DD, Pirani CL, Soothill JF, Muehrcke RC, Kark RM. Diabetic nephropathy: a clinical and pathologic study based on renal biopsies. Medicine 1959;38:312–367.Google Scholar