Brain Tumor Pathology

, Volume 18, Issue 1, pp 7–12 | Cite as

Expression of hepatocyte growth factor and matrix metalloproteinase-2 in human glioma

  • Hirohito Yano
  • Akira Hara
  • Satoru Murase
  • Katsuhiko Hayashi
  • Hiromichi Ando
  • Jun Shinoda
  • Kuniyasu Shimokawa
  • Noboru Sakai
Original Article


Hepatocyte growth factor (HGF) has a stimulatory effect on the synthesis of matrix metalloproteinase-2 (MMP-2), which is involved in glioma invasion. In this study, to clarify the correlation between the expression of HGF and MMP-2 in glioma tissues, immunohistochemical analysis of HGF and MMP-2 was performed in 11 cases of astrocytoma, 10 cases of anaplastic astrocytoma, and 9 cases of glioblastoma. As a result, expression of HGF and MMP-2 was correlated with the grade of malignancy (P=0.0181 and 0.0001, respectively), and a significant correlation between the immunoreactivity of HGF and that of MMP-2 was observed (P<0.05). Immunofluorescence study revealed the concomitant expression of HGF and MMP-2 in glioma tissue. In cultured glioma cell lines (SNB-19, U87MG, and U373MG), exogenous recombinant HGF effectively acted on the production of the active and latent forms of MMP-2 protein in a dose-dependent manner. Active MMP-2 increased more effectively than the latent form. Taken together, these results suggest that HGF may promote glioma invasion in vivo by production of MMP-2.

Key words

Hepatocyte growth factor Matrix metalloproteinase-2 Glioma Immunohistochemistry 


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Copyright information

© The Japan Society of Brain Tumor Pathology 2001

Authors and Affiliations

  • Hirohito Yano
    • 1
  • Akira Hara
    • 2
  • Satoru Murase
    • 1
  • Katsuhiko Hayashi
    • 1
  • Hiromichi Ando
    • 1
  • Jun Shinoda
    • 1
  • Kuniyasu Shimokawa
    • 3
  • Noboru Sakai
    • 1
  1. 1.Department of NeurosurgeryGifu University School of MedicineGifuJapan
  2. 2.Department of PathologyGifu University School of MedicineGifuJapan
  3. 3.Department of Laboratary MedicineGifu University School of MedicineGifuJapan

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