Brain Tumor Pathology

, Volume 14, Issue 1, pp 5–11 | Cite as

Neuronal differentiation of human neuroblastoma SH-SY5Y cells by gangliosides

  • Min-Cheol Lee
  • Bong-Woon Kim
  • Jong-Soon Kim
  • Ji-Shin Lee
  • Kyung-Soo Kim
  • Jae-Hyuk Lee
  • Jong-Hee Nam
  • Sung-Man Rowe
  • Seung-U. Kim
Original Article


Exogenous administration of gangliosides induced neuronal differentiation with prominent neuritogenesis in human neuroblastoma SH-SY5Y cells in vitro. Neuritogenesis was characterized by ruffling of the cell membrane, the development of lamellipodia and filopodia, and the subsequent elongation and branching of the neurites ultrastructurally. Both axons and neurites were identified. Increased numbers of cell organelles in the neurites and cell bodies were noted. Nonsynaptic contacts and gap junctions formed between neurites or between each neurite and cell body. These findings could be implicated in histopathologic changes from neuroblastoma to ganglioneuroblastoma.

Key words

Gangliosides Neuronal differentiation Neuroblastoma Ganglioneuroblastoma Ultrastructure 


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Copyright information

© The Japan Society of Brain Tumor Pathology 1997

Authors and Affiliations

  • Min-Cheol Lee
    • 1
  • Bong-Woon Kim
    • 2
  • Jong-Soon Kim
    • 1
  • Ji-Shin Lee
    • 1
  • Kyung-Soo Kim
    • 1
  • Jae-Hyuk Lee
    • 1
  • Jong-Hee Nam
    • 1
  • Sung-Man Rowe
    • 2
  • Seung-U. Kim
    • 3
  1. 1.Department of PathologyChonnam National University Medical School and Medical CenterKwangjuKorea
  2. 2.Department of Rehabilitation MedicineChonnam National University Medical School and Medical CenterKwangjuKorea
  3. 3.Department of MedicineUniversity of British ColumbiaVancouverCanada

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