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A versatile and reproducibly operating microelectrode puller

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Abstract

A microelectrode puller is described with the following properties: reproducibility of pulling; easy adjusting procedure; the possibility of shaping the electrode; and the possibility of intermittent pulling of different types of electrodes. These properties are obtained by: a well phased pulling scheme; nearly frictionless moving parts; symmetrical and vertical pulling; and fully electronic switching.

Sommaire

On décrit un extracteur de microélectrodes ayant les caractéristique suivantes: reproductivité de l'extraction, réglage facile, possibilité de former l'electrode, possibilité d'extraction intermittente des différents types d'électrodes. Ces caractéristiques sont obtenues par: un système d'extraction bien phasé, des pièces mobiles presque sans frottement, l'extraction symétrique et verticale, et une commutation intégralement électronique.

Zusammenfassung

Es wird ein Mikroelektroden-Ziehgerät mit folgenden Merkmalen beschrieben: Reproduzierbarkeit des Ziehvorganges, leichte Einstellung. Möglichkeit, der Elektrode eine Form zu geben, und evtl. intermittierendes Ziehen von Elektroden verschiedener Art. Diese Merkmale werden erreicht durch: ein gut abgestimmtes Ziehsystem, fast reibungslos bewegliche Teile, symmetrisches und senkrechtes Ziehen sowie vollelektronisches Schalten.

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References

  • Baker, F. L. andYork, D. H. (1971) A reliable technique for making glass microelectrodes of known resistance.Proc. IEEE,59, 1711–1712.

    Article  Google Scholar 

  • Brown, K. T. andFlaming, D. G. (1974) Bevelling of fine micropipette electrodes by a rapid precision method.Science 185, 693–695.

    Google Scholar 

  • Chowdhury, T. K. (1969) Fabrication of extremely fine micropipette electrodes.J. Sci. Instrum. S2V2, 1087–1090.

    Google Scholar 

  • Frank, F. andBecker, M. C. (1964) Microelectrodes for recording and stimulation. In:W. L. Nastuk (ed.)Physical techniques in biological research, vol. V, pp. 22–87, Academic Press, New York and London.

    Google Scholar 

  • Fry, D. M. (1975) A scanning electron microscope method for the examination of glass microelectrode tips either before or after use.Experientia 31, 695–697.

    Article  Google Scholar 

  • Hébert, N. C. (1969) Properties of microelectrode glasses. In:M. Lavallée, O. P. Schanne andN. C. Hébert (eds.)Glass microelectrodes, 25–31, J. Wiley.

  • Ling, G. N. andGerard, R. W. (1949) The normal membrane potential of frog sartorius muscle.J. Cellular Comp. Physiol. 34, 383–396.

    Article  Google Scholar 

  • Okada, Y. andInouye, A. (1975) Tip potential and fixed charges on the glass wall of microelectrode.Experientia 31, 545–546.

    Article  Google Scholar 

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Siegenbeek van Heukelom, J., Dijkstra, K. & van Ingen, H. A versatile and reproducibly operating microelectrode puller. Med. & biol. Engng. 14, 644–652 (1976). https://doi.org/10.1007/BF02477042

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  • DOI: https://doi.org/10.1007/BF02477042

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