Abstract
Studies reported here showed that the incubation medium used for frog muscle contains factors able to modulate the secretion of mediator from motor nerve terminals, with increases in release from low-efficiency synapses and decreases in release from high-efficiency synapses. Both the stimulatory and the inhibitory effects of the incubation medium are mediated by the corresponding changes in the number of available mediator quanta stored. Factors with stimulatory presynaptic actions were present mainly in the low-molecular-weight (<10 kDal) fraction of the incubation medium. The extent of the stimulatory action of this fraction on low-efficiency synapses correlated with the concentration of histidine-containing substances.
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References
T. A. Goryukhina, “Estimation of histidine and urocanic acid by paper chromatography,”Ukr. Biokhim. Zh.,31, No. 1, 138–143 (1959).
M. G. Dobretsov, “Conditions for detecting the positive and negative effects of hyperkalemic solutions on neuromuscular synapses,” in:Vest. LGU (Reports of Leningrad State University), Biology Series [in Russian], deposited in the All-Union Institute for Scientific and Technical Information (VINITI), No. 4085-Vyu (1986).
T. M. Drabkina, “The effects of various muscle metabolites on neuromuscular transmission and muscle exhaustion,”Ros. Fiziol. Zh. im. I. M. Sechenova,80, No. 9, 52–60 (1994).
A. L. Zefirov and E. L. Stolov, “Analysis of factors limiting the use of binomial statistics for studies of mediator secretion in neuromuscular synapses,”Neirofiziologiya,16, No. 2, 182–189 (1984).
B. Katz,Nerve, Muscle, and Synapse [in Russian], Moscow (1968).
A. V. Kibyakov, “Humoral factors in the interaction between nerves and muscles,” in:Studies of the Tatar Institute of Theoretical and Clinical Medicine [in Russian], Kazan' (1935), pp. 141–148.
I. I. Krivoi, I. V. Kubasov, and E. V. Lopatina “Studies of the functional recovery of exhausted rat diaphragm after application of exogenous acetylcholine,”Ros. Fiziol. Zh. im. I. M. Sechenova,80, No. 9, 61–66 (1994).
D. P. MatyushkinFunctional Cellular Interactions in the Neuromuscular Apparatus [in Russian], Leningrad (1980).
D. P. Matyushkin,Feedback in Synapses [in Russian], Nauka, Leningrad (1989).
D. P. Matyushkin, T. M. Drabkina, and I. A. Shabunova, “Quantitative assessment of presynaptic apparatus function in single and multiple synapses,”Usp. Fiziol. Nauk,11, No. 2, 49–70 (1980).
D. P. Matyushkin, T. M. Drabkina, and I. A. Shabunova, “The question of the involvement of various muscle metabolites in the antidromal regulation of mediator release from motor nerve terminals,”Vestn. LGU, Ser 3, No. 2, 76–83 (1986).
A. A. Mokrushin and I. V. Karpova, “The effects of perfusates from tetanized donor slices on the induction of long-term potentiation in recipient slices,”Ros. Fiziol. Zh. im. I. M. Sechenova,80, No. 4, 8–12 (1994).
A. A. Mokrushin and A. V. Tokarev, “Endogenous regulators of long-term potentiation and depression in rat olfactory cortex slices,”Ros. Fiziol. Zh. im. I. M. Sechenova,81, No. 8, 39–44 (1995).
E. E. Nikol'skii and G. I. Poletaev, “Studies of the mechanisms of blockade of transmission from nerve to skeletal muscle in conditions of infrequent neuronal stimulation,”Neirofiziologiya,9, No. 1, 78–85 (1977).
D. Yu. Romanovskii and T. M. Drabkina, “The release of various muscle metabolites into the incubation and perfusion media from frog skeletal muscle,”Vestn. SPbGU, Ser. 3, No. 1, 75–80 (1995).
I. A. Shabunova, “The release of histidine from frog skeletal muscle at rest and in concentrations of rhythmic activity,”Fiziol. Zh. SSSR,65, No. 9, 1371–1373 (1979).
T. M. Drabkina, D. P. Matyushkin, V. K. Radzjukevich, and D. Yu. Romanovsky, “The influence of skeletal muscle incubation medium on fatigue of neuromuscular preparation and on transmitter release at neuromuscular junctions in the frog,”Gen. Physiol. Biophys.,14, No. 2, 1–18 (1995).
D. Elmqvist and D. M. J. Quastel, “A quantitative study of end-plate potentials in isolated human muscles,”J. Physiol. (London),178, No. 2, 505–529 (1965).
A. J. Harborn, J. K. Shute, and M. E. Smith, “Release of hydrolases and acetylcholine sensitivity in rat skeletal muscle,”J. Physiol. (London),352, 385–393 (1984).
J. E. Heuser and T. S. Reese, “Evidence for recycling of synaptic vesicle membrane during transmitter release at the frog neuromuscular junction,”J. Cell. Biol.,57, No. 6, 315–344 (1973).
O. N. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, “Protein measurement with the Folin phenol reagent,”J. Biol. Chem.,193, No. 1, 265–275 (1951).
D. P. Matyushkin, I. I. Krivoi, T. M. Drabkina, “Synaptic feed-back mediated by potassium ions,”Gen. Physiol. Biophys.,14, No. 2, 369–381 (1995).
J. Ovadi, S. Libor, and P. Elodi, “Spectrophotometric determination of histidine in proteins with diethylpyrocarbonate,”Acta Biochim. Biophys. Acad. Sci. Hung.,2, No. 4, 455–458 (1967).
P. Schotman, L. H. Schrama, and Ph. M. Edwards, “The peptidergic system,” in:Handbook of Neurochemistry Plenum Press, New York, London (1985), Vol. 8.
B. Williams and K. Wilson, “Methods of chromatography,” in:Priciples and Technique of Practical Biochemistry, Arnold, London (1975).
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Translated from Rossiiskii Fiziologicheskii Zhurnal imeni I. M. Sechenov, Vol. 85, No. 1, pp. 149–158, January, 1999.
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Drabkina, T.M., Matyushkin, D.P. & Romanovskii, D.Y. The modulating effects of various muscle factors on the function of motor nerve terminals. Neurosci Behav Physiol 30, 365–372 (2000). https://doi.org/10.1007/BF02471791
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DOI: https://doi.org/10.1007/BF02471791