Abstract
This report describes the results of a comparative genetic analysis of the proliferation activity, level of chromosomal aberrations, and the state of interphase chromatin in nerve cells from the developing brains of rat embryos from lines with nervous systems of different excitation thresholds. A relationship between the basal level of nervous system excitability and these cytogenetic characteristics was found. Higher values were seen in embryos of the high-threshold line VP1, as compared with the low-threshold line NP1. The role of the cytogenetic characteristics of the embryo brain in the processes of CNS development and function are discussed in relation to the genetically determined level of nervous system excitability.
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B. Alberts, D. Bray, J. Lewis, M. Reff, K. Roberts, and J. Watson,Molecular Biology of the Cell, Vol. 2, Ch. 8,The Cell Nucleus, section 8.5,Mechanisms of Genetic Control and Cellular Memory [Russian translation], Mir, Moscow (1986), pp. 282–294.
N. V. Bykovskaya, N. A. Dyuzhikova, A. I. Vaido, N. G. Lopatina, and P. Ya. Shvartsman, “The frequency of chromosomal aberrations induced by stress and cyclophosphamide in bone marrow cells from rats selected for nervous system excitability thresholds,”Genetika,30, No. 9, 1224–1228 (1994).
A. I. Vaido and M. Kh. Sitdikov, “Selection of rat lines for long-term excitation thresholds in the neuromuscular apparatus,”Genetika,15, No. 1, 144–147 (1979).
V. V. Vshivtseva, A. I. Vaido, N. A. Dyuzhikova, and N. V. Shiryaeva, “Morphological differences in brain development in rat lines with different levels of nervous system excitability,” in:Proceedings of the Third International Conference of CIS Countries on Functional Neuromorphology, “Kolosov Readings 97” [in Russian], (1997), p. 25.
N. I. Dmitrieva and St. Gotstso, “Structural characteristics of the brains of rats selected for neuromuscular excitability thresholds,”Arkh. Anat. Gist. Émbriol.,88, No. 7, 5–10 (1985).
N. A. Dyuzhikova, N. V. Bykovskaya, A. I. Vaido, N. V. Shiryaeva, N. G. Lopatina, and P. Ya. Shvartsman, “The frequency of chromosomal lesions induced by single exposures to stress in rats selected for nervous system excitability,”Genetika,32, No. 6, 851–853 (1996).
N. A. Dyuzhikova and M. B. Pavlova, “Sensitivity of the chromosomal apparatus to stress in rats with different levels of nervous system excitability,” in:Proceedings of the First All-Russian Conference of Young Physiologists and Biochemists [in Russian], Leningrad (1995).
V. A. Ivanov and N. A. Tushmalova, “DNA synthesis and higher brain functions,”Usp. Sovrem. Biol.,106, No. 5, 163–178 (1988).
L. I. Lebedeva, S. V. Skorova, and E. M. Akhmamet'eva, “Possible mechanisms for the generation of chromosomal rearrangements. VI. Delayed mitosis as a protective mechanisms. The origin of spontaneous chromosomal breaks,”Genetika,29, No. 11, 1826–1830 (1993).
L. I. Lebedeva and N. N. Yakovchenko “Possible mechanisms for the generation of chromosomal rearrangements. IV. Chromosomal rearrangements in spontaneous mutagenesis,”Genetika,24, No. 6, 1033–1040 (1988).
N. G. Lopatina and V. V. Ponomarenko, “Studies of the genetic bases of higher nervous activity,” in:The Physiology of Behavior. Neurobiological Aspects [in Russian], A. S. Batuev (Ed.), Nauka, Leningrad (1987), pp. 9–59.
L. A. Mamon and Yu. A. Kutskova, “The role of heat shock proteins in repairing damage to mitotic chromosomes induced by high temperature inD. melanogaster,”Genetika,29, No. 4, 604–612 (1993).
A. V. Medvedeva and E. V. Savvateeva, “The effects of ats-mutation in theagnostic gene, which controls the funciton of calmodulin and learning ability, on the ectopic conjugation of polytene chromosomes inDrosophila,”Dokl. Adak. Nauk SSSR,318, No. 3, 733–736 (1991).
A. V. Medvedeva and E. V. Savvateeva, “The effects of temperature on the spatial organization of polytene chromosomes inDrosophila mutants with altered calmodulin function,”Dokl. Adak. Nauk SSSR,318, No. 4, 988–991 (1991).
A. V. Medvedeva, E. V. Tokmacheva, E. V. Savvateeva, and V. S. Kornitskii, “Characteristics of genome organization inDrosophila mutants with altered second messenger metabolism and learning ability,”Ros. Fiziol. Zh. im. I. M. Sechenova,81, No. 8, 90–93 (1995).
F. Z. Meerson and I. Yu. Malyshev,The Phenomenon of Adaptive Stabilization of Structures and the Protection of the Heart [in Russian], Nauka, Moscow (1993).
V. V. Ponomarenko, “Some molecular and systems aspects of the genetic control of behavior,” in:Proceedings of the 11th Congress of the I. P. Pavlov All-Russian Physiology Society [in Russian], Leningrad (1970), pp. 97–101.
A. A. Prokof'eva-Bel'govskaya,The Heterochromatin Regions of Chromosomes [in Russian], Moscow (1986).
K. Yu. Reznikov,Proliferation of Vertebrate Brain Cells in Conditions of Normal and Traumatic Development of the Brain [in Russian], Nauka, Moscow (1981).
K. Yu. Reznikov and G. D. Nazarevskaya,Proliferation and Cytogenesis in the Developing Hippocampus, [in Russian], Nauka, Moscow (1989).
E. V. Savvateeva, “The genetic control of second messenger systems and their role in learning,”Usp. Sovrem. Genet.,17, 33–99 (1991).
E. V. Savvateeva, L. M. Sharagina, and N. G. Lopatina, “Phosphodiesterase activity and the activational properties of calmodulin extracted from the hippocampus and other parts of the brains of rat lines selected for nervous system excitability and differing in terms of long-term potentiation, learning ability, and stress responses,”Biol. Membrany,8, 1185–1186 (1991).
E. V. Tokmacheva “Studies of the mitotic activity of cells in the major neural ganglion of larvae of ats-mutant ofDrosophila with altered learning ability and increased calmodulin activational properties,”Zh. Vyssh. Nerv. Deyat,45, No. 3, 565–571 (1995).
N. V. Shiryaeva, A. I. Vaido, N. G. Lopatina, D. A. Kulagin, T. S. Glushchenko, and N. P. Taranova, “Differential sensitivity to induction of neurosis of rat lines selected for nervous system excitability threshold,”Zh. Vyssh. Nerv. Deyat.,42, No. 1, 137–143 (1992).
L. Bret, M. Delverdier, and G. F. Fourine, “L'apoptose,”Rev. Med. Vet.,M4, No. 7, 579–589 (1993).
C. E. Ford and J. L. Hamilton, “A colchicine, hypotonic citrate, citrate squash sequence for mammalian chromosomes,”Stain Technol.,31, 277 (1956).
E. V. Savvateeva and N. G. Kamyshev, “Behavioral effects of temperature-sensitive mutations affecting metabolism of cAMP inDr. melanogaster,”Pharmacol. Biochem. Behav.,14, 603–611 (1981).
D. L. Spector, “Nuclear organization and gene expression,”Exp. Cell Res.,229, No. 2, 189–198 (1996).
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Translated from Rossiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 85, No. 1, pp. 93–98, January, 1999.
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Dyuzhikova, N.A., Shiryaeva, N.V., Vaido, A.I. et al. Proliferative activity and characteristics of the structural-functional organization of the chromosomes in cells of the developing brain, related to the genetically determined excitability of the nervous system in rats. Neurosci Behav Physiol 30, 315–319 (2000). https://doi.org/10.1007/BF02471784
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DOI: https://doi.org/10.1007/BF02471784