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Human breast carcinoma (ZR-75-1) serially transplanted into nude mice-with reference to estradiol dependency and sensitivity to tamoxifen

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Abstract

Tumor cells of the ZR-75-1 line (1×107 in 0.5 ml) were inoculated into the right thigh muscles of BALB/c female nude mice which were, at the same time, given 1.0 mg of estradiol subcutaneously. After the transferable strain had been established, the tumors were then transplanted into female and male nude mice either with or without estradiol treatment. Although no exponential tumor growth was observed in the untreated male and female nude mice, no complete rejection was found during the experiments. The estrogen receptor of this strain was positive and the growth of ZR-75-1 was dependent on exogenous estradiol. ZR-75-1 in the nude mouse was insensitive to tamoxifen treatment, given as 5 mg/kg intramuscularly twice a week, suggesting that dependency on estradiol is not necessarily correlated with endocrine sensitivity to tamoxifen.

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References

  1. Kubota T, Kubouchi K, Koh J, Enomoto K, Ishibiki K, Abe O. Human breast carcinoma (MCF-7) serially transplanted into nude mice. Jpn J Surg 1983; 13: 381–383.

    Article  PubMed  CAS  Google Scholar 

  2. Hirohashi S, Shimosato Y, Kameya T, Nagai K, Tsunematsu R. Hormone dependency of a serially transplantable human breast cancer (Br-10) in nude mice. Cancer Res 1977; 37: 3184–3189.

    PubMed  CAS  Google Scholar 

  3. Brünner N, Spang-Thomsen M, Skovgaard Poulsen H, Engelholm A, Nielsen A, Vindeløv L. Endocrine sensitivity of receptor-positive T61 human breast carcinoma serially grown in nude mice. Int J Cancer 1985; 35: 59–64.

    PubMed  Google Scholar 

  4. Lippman ME, Osborne CK, Knazek R, Young N.In vitro model systems for the study of hormone-dependent human breast cancer. New Engl J Med 1977; 296: 154–159.

    Article  PubMed  CAS  Google Scholar 

  5. Kubota T, Fukutomi T, Isobe Y, Kikuyama S, Ishibiki K, Abe O. Experimental endocrinotherapy of hormone-dependent human breast carcinomas transplanted into nude mice. In: Rygaard J, Brünner N, Gram N, Spang-Thomsen M, eds. Immune-deficient animals in biomedical research. Basel: Karger 1987; 352–355.

    Google Scholar 

  6. Geran RI, Greenberg NH, MacDonald MM, Schumacher AM, Abbott BJ. Protocols for screening chemical agents and natural products against animal tumors and other biological systems (third edition). Cancer Chemother Rep 1972; Part 3, 3: 51–61.

    Google Scholar 

  7. Kubota T, Nakada M, Tsuyuki K, Inada T, Asanuma F, Ishibiki K, Abe O. Cell kinetics and chemosensitivity of human carcinomas serially transplanted into nude mice. Jpn J Cancer Res (Gann) 1986; 77: 502–507.

    CAS  Google Scholar 

  8. Fukutomi T, Kubota T, Ikeda T, Isobe Y, Kikuyama S, Shimada A, Nakamura A, Enomoto K, Ishibiki K, Abe O. Experimental combined hormone therapy on human breast carcinomas serially transplanted into nude mice. Jpn J Cancer Res (Gann) 1986; 77: 92–97.

    CAS  Google Scholar 

  9. Yamasaki T. Estrogen receptor in malignant and benign breast tumors and normal tissues of Japanese patients. Jpn J Clin Oncol 1978; 37–47.

  10. Nawata H, Bronzert D, Lippman ME. Isolation and characterization of a tamoxifen-resistant cell line derived from MCF-7 human breast cancer cells. J Biol Chem 1981; 256: 5016–5021.

    PubMed  CAS  Google Scholar 

  11. Koh J. Experimental chemo and chemoendocrine therapy of human breast carcinomas serially transplanted into nude mice. Jpn J Surg 1988; 89: 91–102. (in Japanese)

    CAS  Google Scholar 

  12. Fukutomi T, Ikeda T, Nishiumi T, Kikuyama S, Isobe Y, Nakamura A, Shimada M, Inada T, Ishii S, Koh J, Enomoto K, Ishibiki K, Abe O, Kubota T. Effect of hydroxy tamoxifens against human breast carcinomas transplanted into nude mice. J Jpn Soc Cancer Ther 1986; 21: 50–59. (in Japanese with English Abst.)

    CAS  Google Scholar 

  13. McGuire WL, Horwitz KB, Zava DT, Garola RE, Chamness GC. Progress in endocrinology and metabolism: Hormones in breast cancer: Update 1978. Metabolism 1978; 27: 487–501.

    Article  PubMed  CAS  Google Scholar 

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Kubota, T., Oka, S., Utsumi, T. et al. Human breast carcinoma (ZR-75-1) serially transplanted into nude mice-with reference to estradiol dependency and sensitivity to tamoxifen. The Japanese Journal of Surgery 19, 446–451 (1989). https://doi.org/10.1007/BF02471626

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