Abstract
An association between DNA aneuploidy of tumor cells and a poorer clinical outcome of patients has been recognized in various human solid tumors. In this article, the prognostic value of flow cytometric DNA analysis in colorectal cancer patients is briefly overviewed. DNA aneuploidy appeared to correlate with more advanced disease and a poorer survival in colorectal cancer patients, but reported results were not always consistent. DNA ploidy as a marker for predicting the survival of colorectal cancer patients should not therefore be viewed in isolation, but should be evaluated in combination with other conventional prognostic variables. The S-phase cell compartment within a tumor appears to be promising as a marker for predicting the survival of colorectal cancer patients, but there remain technical problems in establishing an accurate estimation of the S-phase cell compartment within a tumor by conventional flow cytometry. The application of a bromodeoxyuridine (BrdU)-specific monoclonal antibody might be an useful tool for obtaining the accurate flow cytometric estimation of the S-phase cell population within a tumor.
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Barlogie B, Raber MN, Schumann J, Johnson TS, Drewinko B, Swartzendruber DE, Goehde W, Andreeff M, Freireich EJ. Flow cytometry in clinical cancer research. Cancer Res 1983; 43: 3982–3997.
Friedlander ML, Hedley DW, Taylor IW. Clinical and biological significance of aneuploidy in human tumors. J Clin Pathol 1984; 37: 961–974.
Merkel DE, Dressler LG, McGuire WL. Flow cytometry, cellular DNA content, and prognosis in human malignancy. J Clin Oncol 1987; 5: 1690–1703.
Quirke P, Dyson JED, Dixon MF, Bird CC, Joslin CAF. Heterogeneity of colorectal adenocarcinomas evaluated by flow cytometry and histopathology. Br J Cancer 1985; 51: 99–106.
Hiddemann W, von Bassewitx DB, Klienmeier H, Shulte-Brochterbeck E, Hauss J, Lingemann B, Buechner T, Grundmann E. DNA stemline heterogeneity in colorectal cancer. Cancer 1986; 58: 258–263.
Scott NA, Crande JP, Weiland LH, Pembertson JH, Beart RW, Lieber MM. Flow cytometric DNA patterns from colorectal cancers: How reproducible are they? Mayo Clin Proc 1987; 62: 331–337.
Wolley RC, Schreiber K, Koss LG, Karas M, Sherman A. DNA distribution in human colon carcinomas and its relationship to clinical behavior. JNCI 1982; 69: 15–22.
Hedley DW, Friedlander ML, Taylor IW. Application of DNA flow cytometry to paraffin-embedded archival material for the study of aneuploidy and its clinical significance. Cytometry 1985; 6: 327–333.
Quirke P, Dixon MF, Claydon AD, Durdey P, Dyson JD, Williams NS, Bird C. Prognostic significance of DNA aneuploidy and cell proliferation in rectal adenocarcinomas. J Pathol 1987; 151: 285–291.
Armitage NC, Robins RA, Evans DF, Turner DR, Baldwin RW, Hardcastle JD. The influence of tumor cell DNA abnormalities on survival in colorectal cancer. Br J Surg 1985; 72: 828–830.
Emdin SO, Steinling R, Roos G. Prognostic value of DNA content in colorectal carcinoma. A flow cytometric study with some methodologic aspects. Cancer 1987; 60: 1282–1287.
Scott NA, Rainwater LM, Wieand HS, Weiland LH, Pemberton JH, Beart RW, Lieber MM. The relative prognostic value of flow cytometric DNA analysis and conventional clinicopathologic criteria in patients with operable rectal carcinoma. Dis Colon Rectum 1987; 30: 513–520.
Chang KJ, Enker WE, Melamed M. Influence of tumor cell DNA ploidy on the natural history of rectal cancer. Am J Surg 1987; 153: 184–188.
Melamed MR, Enker WE, Banner P, Janov AJ, Kessler G, Darzynkiewicz Z. Flow cytometry of colorectal carcinoma with three-year follow-up. Dis Colon Rectum 1986; 29: 184–186.
Finan PJ, Quirke P, Dixon MF, Dyson JED, Giles GR, Bird CC. Is DNA aneuploidy a good prognostic indicator in patients with advanced colorectal cancer? Br J Cancer 1986; 54: 327–330.
Suzuki H, Matsumoto K, Masuda T, Koike H. DNA ploidy of colorectal carcinoma: Correlation with conventional prognostic variables. J Clin Gastroenterol 1988; 10: 176–178.
Kokal W, Sheibani K, Terz J, Harada JR. Tumor DNA content in the prognosis of colorectal carcinoma. JAMA 1986; 255: 3123–3127.
Goh HS, Jass JR, Atkin WS, Cuzick J, Northover JMA. Value of flow cytometric determination of ploidy as guide to prognosis in operable rectal cancer: A multivariate analysis. Int J Colorect Dis 1987; 2: 17–21.
Olszewski W, Darzynkiewicz Z, Rosen PP, Schwartz MK, Melamed MR. Flow cytometry of breast carcinoma: II. Relation of tumor cell cycle distribution to histology and estrogen receptor. Cancer 1981; 48: 985–988.
Suzuki H, Koike H. Prognostic significance of DNA ploidy and proliferative index in breast carcinoma. Arch Surg 1988; 123: 783.
Volm M, Bruggeman A, Gunther M, Kleine W, Pfleiderer A, Vogt-Schaden M. Prognostic significance of ploidy, proliferation, and resistance-predictive tests in ovarian carcinoma. Cancer Res 1985; 45: 5180–5185.
Gansler T, Chatten J, Varello M, Bunin GB, Atkinson B. Flow cytometric DNA analysis of neuroblastoma: Correlation with histology and clinical outcome. Cancer 1986; 58: 2453–2458.
Barlogie B, Goehde W, Johnston DA, Smallwood L, Schumann J, Drewinko B, Freireich EJ. Determination of ploidy and proliferative characteristics of human solid tumors by pulse cytophotometry. Cancer Res 1978; 38: 3333–3339.
Dolbeare FD, Gratzner H, Pallavicini MG, Gray JW. Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. Proc Natl Acad Sci USA 1983; 80: 5573–5577.
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Suzuki, H. The prognostic value of flow cytometric DNA analysis in colorectal cancer patients. The Japanese Journal of Surgery 18, 483–486 (1988). https://doi.org/10.1007/BF02471479
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DOI: https://doi.org/10.1007/BF02471479