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Gallbladder bile peptides and their structure

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Abstract

Human gallbladder bile was separated by salting-in column chromatography using silica gel ammonium sulfate system. The second fraction containing peptides was found to vary in cholelithiasis in relation to Fraction 1.

To determine the structure of the peptides, bovine gallbladder bile was fractionated by salting out method with ammonium sulfate. Four peptides were isolated and their amino acid sequences were determined to be:

  1. 1)

    β-Ala-(Trp1, His1, Ileu1, Phe1)

  2. 2)
  3. 3)

    Tyr-Gly

  4. 4)

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References

  1. Bock, R. M. and Ling, N. S.: Devices for gradient elution in chromatography. Anal. Chem., 26: 1543–1546, 1954.

    Article  CAS  Google Scholar 

  2. Fraenkel-Conrat, H., Harris, J. I. and Levy, A. L.: Methods of Biochemical Analysis, 2: 359–425, Interscience Publishers, New York, 1955.

    Google Scholar 

  3. Hill, R. L. and Smith, E. L.: Leucine aminopeptidase VII. Action on long chain polypeptides and proteins. J. Biol. Chem., 228: 577–600, 1957.

    PubMed  CAS  Google Scholar 

  4. Katsuki, T.: Studies on bile proteins. J. Jap. Surg. Soc., 57: 1643–1662, 1957 (in Japanese with English summary).

    Google Scholar 

  5. Konigsberg, W. and Hill, R. J.: The structure of human hemoglobin. III The sequence of amino acids in the tryptic peptides of the α-chain. J. Biol. Chem., 237: 2547–2561, 1962.

    PubMed  CAS  Google Scholar 

  6. Konomi, K.: The occurrence of peptides in human gallbladder bile. Fukuoka Acta Med., 56: 26–52, 1965 (in Japanese with English summary).

    CAS  Google Scholar 

  7. Levy, A. L.: A paper chromatographic method for the quantitative estimation of amino-acids. Nature, 174: 126–129, 1954.

    Article  PubMed  CAS  Google Scholar 

  8. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193: 265–275, 1951.

    PubMed  CAS  Google Scholar 

  9. Miyahara, K.: Studies on peptides containing allo-γ-hydroxyglutamic acid in human gall-bladder bile. Igaku Kenkyu, 44: 438–448, 1974 (in Japanese with English summary).

    PubMed  CAS  Google Scholar 

  10. Shimura, H.: Purification of bile protein. Hirosaki Med. J., 8: 435–457, 1957 (in Japanese with English summary).

    CAS  Google Scholar 

  11. Spackman, D. H., Stein, W. H. and Moore, S.: Automatic recording apparatus for use in the chromatography of amino acids. Anal. Chem., 30: 1190–1206, 1958.

    Article  CAS  Google Scholar 

  12. Vershure, J. C. M., Mijnlieff, P. F.: The dominating macromolecular complex of human gallbladder bile, clin. Chim. Acta, 1: 154–166, 1956.

    Article  Google Scholar 

  13. Waley, S. G. and Watson, J.: The action of trypsine on polylysine. Biochem. J., 55: 328–336, 1953.

    PubMed  CAS  Google Scholar 

  14. Yamamoto, H.: Studies on bile peptides. Igaku Kenkyu, 39: 117–125, 1969 (in Japanese with English summary).

    Google Scholar 

  15. Yamamoto, H., Funatsu, G. and Funatsu, M.: Isolation and structure of a peptide containing Allo-γ-hydroxyglutamic acid from bovine gallbladder bile. Proc. Jap. Acad., 44: 391–396, 1968.

    CAS  Google Scholar 

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Authors and Affiliations

  1. First Department of Surgery, Kyushu University Faculty of Medicine, 3-1-1 Maidashi, Higashi-Ku, 812, Fukuoka, Japan

    Kohki Konomi, Hiroshi Yamamoto & Masaya Nishimura

Authors
  1. Kohki Konomi
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  2. Hiroshi Yamamoto
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  3. Masaya Nishimura
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Konomi, K., Yamamoto, H. & Nishimura, M. Gallbladder bile peptides and their structure. The Japanese Journal of Surgery 4, 48–55 (1974). https://doi.org/10.1007/BF02471381

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  • DOI: https://doi.org/10.1007/BF02471381

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