Abstract
To clarify the effects of oxygen tension on colony formation of fresh human tumor cells we examined 25 fresh human tumor samples (sixteen gastric cancers, three colon cancers three breast cancers, one esophageal cancer, one leiomyosarcoma and one malignant lymphoma), using the human tumor clonogenic assay (HTCA) technique. Three different oxygen tensions (2 per cent, 5 per cent, 20 per cent) were tested. At 5 per cent O2, which is considered to be physiological oxygen tension, 19 out of 26 tumors (13/16 in gastric cancer, 2/3 in colon cancer, 1/3 in breast cancer, 1/1 in esophageal cancer, and 1/1 in leiomyosarcoma) showed significant increases of plating efficiencies as compared to those at 20 per cent O2. On the other hand, decreases in plating efficiencies were observed at 2 per cent O2 in seven out of 12 tumors as compared to 20 per cent O2 and eight out of 12 tumors, as compared to 5 per cent O2.
This is a preview of subscription content, log in via an institution to check access.
References
Taylor WMG, Richter, A, Evans VJ, Sanford KK. Influence of oxygen and pH on plating efficiency and colon development of WI-38 and Vero cells. Exp Cell Res 1974; 86: 152–156.
Richter A, Sanford KK, Evans VJ. Influence of oxygen and culture media on plating efficiency of some mammalian tissue cells. J Natl Cancer Inst 1972; 49: 1705–1712.
Bradley TR, Hodgson GS, Rosendaal M. The effect of oxygen tension on hemopoietic and fibroblast cell proliferationin vitro. J Cell Physiol 1978; 97: 517–522.
Hamburger AW, Salmon SE. Primary bioassay of human tumor stem cells. Science 1977; 197: 461–463.
Hirabayashi N, Yoshinaka K, Nosoh Y, Toge T, Niimoto M, Hattori T, Ohkita T.In vitro chemosensitivity tests on human tumor xenografts by clonogenic assay: the combined use of mitomycin C with α-interferon or γ-interferon. Jpn J Surgery 1985; 15: 279–284.
Sridhar KS, Plasse TF, Holland JF, Shapiro M, Ohnuma T. Effects of physiological oxygen concentration on human tumor colony growth in soft agar. Cancer Res 1983; 43: 4629–4631.
Jamieson D, Van Den Brenk HAS. Effect of electrode dimensions on tissue pO2 measurementin vivo. Nature 1964; 201: 1227–1228.
Hirst DG, Denekamp J. Tumor cell proliferation in relation to the vasculature. Cell Tissue Kinet 1979; 13: 31–42.
Rofstad EK. Growth and vascular structure of human melanoma xenografts. Cell Tissue Kinet 1984; 17: 91–101.
Teicher BA, Lazo JS, Sartorelli AC. Classification of antineoplastic agents by their selective toxicities toward oxygenated and hypoxic tumor cells. Cancer Res 1981; 41: 73–81.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hirabayashi, N., Nishiyama, M., Yamaguchi, M. et al. Effects of oxygen tension on tumor colony formations assessed by human tumor clonogenic assay. The Japanese Journal of Surgery 16, 148–151 (1986). https://doi.org/10.1007/BF02471086
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02471086