Abstract
Early mucosal changes in dimethylhydrazine (DMH)-induced colonic carcinogenesis were investigated in 12 rats. These rats received subcutaneous injections of DMH in a dose of 15 mg/kg/week. Two rats each were killed at 6, 8, 10, 14, 18 and 22 weeks after initiation of the DMH-treatment. Overt carcinomas developed in 3 out of 4 rats killed after the 18th week of DMH-treatment. Histopathologically, epithelial dysplasia was present in the flat colonic mucosa of all 6 rats killed after the 14th week of DMH-treatment. Neither grossly visible tumors nor histopathological dysplasia was present in the colon of the 6 rats killed before the 14th week of DMH-treatment. However, cytophotometric DNA analysis disclosed that significant increases in proliferative activity of mucosa had occurred 4 weeks before the appearance of histopathological dysplasia, and 8 weeks prior to development of grossly visible tumors. Therefore, changes in the flat mucosa of a tumor-bearing colon represents a primary precancerous condition.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Suzuki H, Honda E, Matsumoto K, Iriyama K. Tissue CEA determination and cytophotometric DNA analysis of colorectal mucosa in patients with colorectal cancer. Jpn J Surg 1985; 15: 449–454.
Otsuji A. Pathological studies on early stage of colon carcinomas in 1,2-dimethylhydrazine carcinogenesis in rats. Kagoshima Daigaku Igaku Zasshi (Med J Kagoshima Univ) 1979; 31: 51–59. (in Japanese)
Hammerberg C, Slezak P, Tribukait B. Early detection of malignancy in ulcerative colitis: A flowcytometric study. Cancer 1984; 53: 291–295.
Muto T, Konishi F. Carcinoma in ulcerative colitis. In: Shiratori T, Nakao H eds., Inflammatory bowel disease. Tokyo: University of Tokyo Press, 1984; 151–160.
Suzuki H, Moriyama S, Matsushima K, Matsumoto K, Iriyama K. Cytophotometric DNA analysis of rectal mucosa of patients with ulcerative colitis. Jpn J Surg 1985; 15: 324–326.
Deschner EE, Lipkin M. Proliferative patterns in colonic mucosa in familial polyposis. Cancer 1975; 35: 413–418.
Romagnoli P, Filliponi F, Bandettini L, Brugnola D. Increase of mitotic activity in the colonic mucosa of patients with colorectal cancer. Dis Colon Rectum 1984; 27: 305–308.
Lev R, Lance P, Camara P. Histochemical and morphologic studies of mucosa bordering rectosigmoid carcinomas: Comparison with normal, diseased, and malignant colonic epithelium. Hum Pathol 1984; 16: 151–161.
Isaacson P, Attwood PRA. Failure to demonstrate specificity of the morphological and histochemical changes in mucosa adjacent to colonic carcinoma (transitional mucosa). J Clin Pathol 1979; 32: 214–218.
Bleiberg H, Buyse M, Galand P. Cell kinetic indicators of premalignant stages of colorectal cancer. Cancer 1985; 56: 124–129.
Ross JS. Experimental large intestinal adenocarcinoma induced by hydrazines and human colorectal cancer: A comparative study. In: Malt RA, Williamson RC eds., Colonic carcinogenesis. Lancaster-Boston-The Hague: MTP Press, 1982; 187–207.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Suzuki, H., Umehara, N. Early mucosal changes in dimethylhydrazine-induced colonic carcinogenesis in rats. The Japanese Journal of Surgery 16, 140–143 (1986). https://doi.org/10.1007/BF02471084
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02471084