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The effect of superoxide dismutase and catalase on myocardial reperfusion injury in the isolated rat heart

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Abstract

This study was designed to evaluate the efficacy of superoxide dismutase (SOD) and catalase on ischemic and reperfusion injury in the isolated working rat heart. The temperature and duration of ischemia varied under three conditions: 1) at 37°C for 35 minutes, 2) at 28°C for 120 minutes and 3) at 20°C for 120 minutes. SOD (100 mg/L) and catalase (10 mg/L) were either added to St. Thomas' Hospital cardioplegic solution during ischemia (CP group) or to the reperfusion solution for 10 minutes after reflow (RS group). They were compared with a control group which received no free radical scavengers. The postischemic recovery ratio of cardiac functions were markedly superior to the values of the control group with a significant difference being noted in the CP and RS groups under ischemia at 37°C and 28°C. In the series done at 20°C, a significant improvement was seen in the RS group, and the CP group also showed better functional recovery rates compared with the control group, although the differences were not statistically significant. Thus, SOD and catalase added to the cardioplegic solution or reperfusion fluid demonstrated an excellent protective effect on the myocardium against ischemic or reperfusion injury in both hypothermic ischemia and normothermia.

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References

  1. Thompson JA, Hess ML. The oxygen free radical system: A fundamental mechanism in the reduction of myocardial necrosis. Prog Cardiovasc Diseases 1986; 28: 449–462.

    Article  CAS  Google Scholar 

  2. Werns SW, Shea MJ, Lucchesi BR. Free radicals and myocardial injury: pharmacologic implications. Circulation 1986; 74: 1–5.

    PubMed  CAS  Google Scholar 

  3. Kim MS, Akera T: O2 free radicals: cause of ischemia-reperfusion injury to cardiac Na+−K+-ATPase. Am J Physiol 1987; 252: H252–257.

    PubMed  CAS  Google Scholar 

  4. Guarnieri C, Flamigni F, Caldarela CM. Role of oxygen in the cellular damage induced by reoxygenation of the hypoxic heart. J Moll Cell Cardiol 1980; 12: 797–808.

    Article  CAS  Google Scholar 

  5. Hess ML, Manson NH. Molecular oxygen: friend and foe. The role of oxygen free radical system on the calcium paradox, the oxygen paradox and ischemic/reperfusion injury. J Moll Cell Cardiol 1984; 16: 969–985.

    CAS  Google Scholar 

  6. Neely JR, Liebermeister H, Battersby EJ, Morgan HE. Effect of pressure development on oxygen consumption by isolated rat heart. Am J Physiol 1967; 212: 804–814.

    PubMed  CAS  Google Scholar 

  7. Oliver IT. A spectro photometric method for the determination of creatine phosphokinase and myokinase. Biochemi J 1955; 61: 116–122.

    CAS  Google Scholar 

  8. Jennings RB, Ganote CE. Structural changes in myocardium during acute ischemia. Circ Res 1974; (Suppl 3) 34&35:III—156—172.

    Google Scholar 

  9. Greenfield DT, Greenfield LJ, Hess ML. Enhancement of crystalloid cardioplegic protection against global normothermic ischemia by superoxide dismutase plus catalase but not diltiazem in the isolated, working rat heart. J Thorac Cardiovasc Surg 1988; 95: 977–813.

    Google Scholar 

  10. Simpson PJ, Michelson JK, Lucchesi BR. Free radical scavengers in myocardial ischemia. Federation Proc 1987; 46: 2413–2421.

    CAS  Google Scholar 

  11. Stewart JR, Blackwell WH, Crute SL, Loughlin V, Greenfield LJ, Hess ML. Inhibition of surgically induced ischemia/reperfusion injury by oxygen free radical scavengers. J Thorac Cardiovasc Surg 1983; 86: 262–272.

    PubMed  CAS  Google Scholar 

  12. Johnson DL, Horneffer PJ, Dinatale JM Jr, Gott VL, Gardner TJ. Freeradical scavengers improve functional recovery of stunned myocardium in a model of surgical coronary revascularization. Surgery 1987; 102: 334–340.

    PubMed  CAS  Google Scholar 

  13. Burton KP, McCord JM, Ghai G. Myocardial alterations due to free-radical generation. Am J Physiol 1984; 246: H776–783.

    PubMed  CAS  Google Scholar 

  14. Kodama K, Hirayama A, Komamura K. Human plasma superoxide dismutase (SOD)-Activity on reperfusion injury with successful thrombolysis in acute myocardial infarction. Jpn J Med 1989; 28: 202–206.

    PubMed  CAS  Google Scholar 

  15. Shlafer M, Kane PF, Kirsh MM. Superoxide dismutase plus catalase enhances the efficacy of hypothermic cardioplegia to protect the globally ischemic reperfused heart. J Thorac Cardiovasc Surg 1982; 83: 830–839.

    PubMed  CAS  Google Scholar 

  16. Shlafer M, Kane PF, Wiggins VY, Kirsh MM. Possible role of cytotoxic oxygen metabolites in the pathogenesis of cardiac ischemic injury. Circulation 1982; 66(Suppl I): I85–92.

    PubMed  CAS  Google Scholar 

  17. Myers CL, Weiss SJ, Kirsh MM, Shepard BM, Shlafer M. Effects of supplementing hypothermic crystalloid cardioplegic solution with catalase, superoxide dismutase, or deferoxamine on functional recovery of globally ischemic and reperfused isolated hearts. J Thorac Cardiovasc Surg 1986; 91: 281–289.

    PubMed  CAS  Google Scholar 

  18. Menasche P, Grousset C, Gauduel Y, Piwnica A. A comparative study of free radical scavengers in cardioplegic solutions. J Thorac Cardiovasc Surg 1986; 92: 264–271.

    PubMed  CAS  Google Scholar 

  19. Zweier JL, Rayburm BK, Flaherty JT, Weisfeldt ML. Recombinant superoxide dismutase reduces oxygen free radical concentrations in reperfused myocardium. J Clin Invest 1987; 80: 1728–1734.

    Article  PubMed  CAS  Google Scholar 

  20. Blaustein AS, Schine L, Brooks WW, Fanburg BL, Bing OH. Influence of exogenously generated oxidant species on myocardial function. Am J Physiol 1986; 250: H595–599.

    PubMed  CAS  Google Scholar 

  21. Werns SW, Shea MJ, Driscoll EM, Cohen C, Abrams GD, Pitt B, Lucchesi BR. The independent effects of oxygen radical scavengers on canine infarct size. Reduction by superoxide dismutase but not catalase. Circ Res 1985; 56: 895–898.

    PubMed  CAS  Google Scholar 

  22. Ataka K, Yamamoto S, Nishikawa Y, Nakamura K. The protective effect of calcium antagonist and free radical scavenger against myocardial ischemic/reperfusion injury in the isolated rat heart. Kobe J Med Sci 1989; 35: 261–276.

    PubMed  CAS  Google Scholar 

  23. Gallagher KP, Buda AJ, Pace D, Green RA, Shlafer M. Failure of superoxide dismutase and catalase to alter size of infarction in conscious dogs after 3 hours of occlusion followed by reperfusion. Circulation 1986; 73: 1065–1076.

    PubMed  CAS  Google Scholar 

  24. Gardner TJ, Stewart JR, Casale AS, Downey JM, Chambers DE. Reduction of myocardial ischemic injury with oxygen-derived free radical scavengers. Surgery 1983; 94: 423–427.

    PubMed  CAS  Google Scholar 

  25. Otani H, Engelman RM, Rousou JA, Breyer RH, Lemeshow S, Das DK. Cardiac performance during reperfusion improved by pretreatment with oxygen free radical scavengers. J Thorac Cardiovasc Surg 1986; 91: 290–295.

    PubMed  CAS  Google Scholar 

  26. Jolly SR, Kane WJ, Baillie MB, Abrams GD, Lucchesi BR. Canine myocardial reperfusion injury. Its reduction by combined administration of superoxide dismutase and catalse. Circ Res 1984; 54: 277–285.

    PubMed  CAS  Google Scholar 

  27. Stewart JR, Crute SL, Loughlin V, Hess ML, Greenfield LJ. Prevention of free radical-induced myocardial reperfusion injury with allopurinol. J Thorac Cardiovasc Surg 1985; 90: 68–72.

    PubMed  CAS  Google Scholar 

  28. Woodward B, Zakaria MNZ. Effect of some free radical scavengers of reperfusion induced arrhythmias in the isolated rat heart. J Mol Cell Cardiol 1985; 17: 485–493.

    PubMed  CAS  Google Scholar 

  29. Burton KP. Superoxide dismutase enhances recovery following myocardial ischemia. Am J Physiol 1985; 248: H637–643.

    PubMed  CAS  Google Scholar 

  30. Nejima J, Knight DR, Fallon JT, Uemura N, Manders WT, Canfield DR, Cohen MV, Vatner SF. Superoxide dismutase reduces reperfusion arrhythmias but fails to salvage regional function or myocardium at risk in conscious dogs. Circulation 1989; 79: 143–153.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. The Second Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Chuo-ku, 650, Kobe, Japan

    Yasuyuki Nishikawa MD, Shinichiro Yamamoto, Keiji Ataka & Kazuo Nakamura

Authors
  1. Yasuyuki Nishikawa MD
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  2. Shinichiro Yamamoto
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  3. Keiji Ataka
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  4. Kazuo Nakamura
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Nishikawa, Y., Yamamoto, S., Ataka, K. et al. The effect of superoxide dismutase and catalase on myocardial reperfusion injury in the isolated rat heart. The Japanese Journal of Surgery 21, 423–432 (1991). https://doi.org/10.1007/BF02470970

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  • DOI: https://doi.org/10.1007/BF02470970

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