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Comparative study on nude mice isotope assay (NM-IA) and subrenal capsule assay (SRCA) sensitivity tests of anticancer agents

The Japanese journal of surgery volume 17, pages 99–103 (1987)Cite this article

Abstract

A comparative study on nude mice isotope assay (NM-IA) and subrenal capsule assay (SRCA) was done to evaluate the usefulness ofin vivo assays for predicting individual tumor sensitivity against anticancer agents. Sixty-one fresh tumor specimens collected at surgery, under sterile conditions, were examined. Mitomycin C (MMC), 5-fluorouracil (5-FU), cyclophosphamide (CPM), adriamycin (ADM) and cis-DDPlatinum (CDDP) were used in both assays. In NM-IA, the tumor sensitivity was determined by the amount of3H-thymidine incorporated into the tumor which had been implanted into subcutaneous spaces of BALB/c nude mice. In the SRCA, the relative increase in weight of the tumor implanted into the subrenal capsular space of ddY mice was determined and measurements made to evaluate the chemosensitivity. Evaluability rates of the trials were 86.9 per cent with both assays and the response rates were 35.8 per cent in NM-IA and 34.0 per cent in SRCA, respectively. Against MMC, 5-FU, CPM, ADM and CDDP, overall consistency rates between the two assays were 77.8 per cent, 88.6 per cent, 72.7 per cent, 81.8 per cent and 68.2 per cent, respectively. In 8 of these 53 evaluated assays, correlations between the results of assays and clinical effects were examined and overall predictive accuracy rates were 87.5 per cent with both assays. Significant differences between these twoin vivo chemosensitivity tests were not evident.

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Affiliations

  1. Department of Surgery, Research Institute for Nuclear Medicine and Biology, Hiroshima University, 1-2-3 Kasumi-cho, Minami-ku, 734, Hiroshima, Japan

    Masahiko Nishiyama MD, Yoshihiro Nosoh, Masahiro Yamaguchi, Naoki Hirabayashi, Tetsuya Toge, Minoru Niimoto & Takao Hattori

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  1. Masahiko Nishiyama MD
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  2. Yoshihiro Nosoh
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  3. Masahiro Yamaguchi
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  4. Naoki Hirabayashi
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  5. Tetsuya Toge
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  6. Minoru Niimoto
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  7. Takao Hattori
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Nishiyama, M., Nosoh, Y., Yamaguchi, M. et al. Comparative study on nude mice isotope assay (NM-IA) and subrenal capsule assay (SRCA) sensitivity tests of anticancer agents. The Japanese Journal of Surgery 17, 99–103 (1987). https://doi.org/10.1007/BF02470648

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  • DOI: https://doi.org/10.1007/BF02470648

Key Words

  • chemosensitivity test
  • nude mice isotope assay
  • subrenal capsule assay