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The Japanese journal of surgery

, Volume 15, Issue 6, pp 471–476 | Cite as

Use of an intravenous branched chain amino acid enriched diet in the tumor-bearing rat

  • Isao Kawamura
  • Hiroshi Sato
  • Shohei Ogoshi
  • George L. Blackburn
Original Articles

Abstract

The purpose of this study was to determine whether a branched chain amino acids (BCAA) enriched intravenous feeding would have an effect on nitrogen retention in the host tissue, without stimulating tumor growth in the malignant tumor bearing animal. NEDH/c rats with an implanted fibrosarcoma were put on one of two total parenteral nutrition regimens, one of an amino acid formula containing 25 per cent BCAA, by weight and the other containing 50 per cent BCAA. Nitrogen retention in the two tumor-bearing groups was similar, although it was 47 per cent greater than observed in the non-tumor bearing animals (pº0.05). Skeletal protein synthesis was significantly reduced by the presence of the tumor in rats on the 25 per cent BCAA formula (from 10.1 per cent/day to 6.1 per cent/day) but was increased to 7.9 per cent/day by the 50 per cent BCAA formula. The BCAA enriched intravenous feeding does not enhance tumor growth and supports skeletal protein synthesis, at least in this experimental model.

Key Words

branched chain amino acid L-[U-14C] tyrosine skeletal protein synthesis 

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References

  1. 1.
    Warren S. The immediate causes of death in cancer. Amer J Med Sci 1932; 184: 610–615.Google Scholar
  2. 2.
    Costa G. Cachexia, the metabolic component of hepatoma bearing rats to total parenteral hyperalimentation and to ad libitum feeding. J Surg Res 1977; 23: 189–195.CrossRefGoogle Scholar
  3. 3.
    Cameron IL, Ackley WJ, Rogers W. Responses of hepatoma bearing rats to total parenteral hyperalimentation and to ad libitum feeding. J Surg Res 1977; 23: 189–195.PubMedCrossRefGoogle Scholar
  4. 4.
    Steiger E, Oram-Smith J, Miller E, Kuo L, Vars HM. Effect of nutrition on tumor growth and tolerance to chemotherapy. J Surg Res 1975; 18: 455–461.PubMedCrossRefGoogle Scholar
  5. 5.
    Nixon DW, Lawson DH, Kutner M, Ansley J, Schwarts M, Heymsfeld SB, Wesley M, Chawla R, Cortwright TH, Rudman D. Hyperalimentation of the cancer patient with protein calorie undernutrition. Cancer Res 1981; 41: 2038–2044.PubMedGoogle Scholar
  6. 6.
    Hersch EN, Gutterman JU, Macelight GM. Several studies of immune competence of patients undergoing chemotherapy for acute leukemia. J Clin Invest 1974; 54: 401–408.CrossRefGoogle Scholar
  7. 7.
    Copeland EM, Daly JM, Ota DM, Dudrick SJ: Nutrition, cancer and intravenous hyperalimentation. Cancer 1979; 43: 2108–2116.PubMedCrossRefGoogle Scholar
  8. 8.
    Daly JM, Dudrick SJ, Copeland EM. Intravenous hyperalimentation: effect on delayed cutaneous hypersensitivity in cancer patients. Ann Surg 1980; 192: 587–592.PubMedCrossRefGoogle Scholar
  9. 9.
    Kawamura I, Moldawer LL, Keenan RA, Batist G, Bothe A, Bistrian BR, Blackburn GL. Altered amino acid kinetics in rats with progressive tumor growth. Cancer Res 1982; 42: 824–829.PubMedGoogle Scholar
  10. 10.
    Blackburn GL, Moldawer LL, Usui S, O'Keefe SJS, Bothe A, Bistrian BR. Branched chain amino acid administration and metabolism during starvation, injury and infection. Surgery 1979; 86: 307–315.PubMedGoogle Scholar
  11. 11.
    Lundholm K, Bylund AC, Holm J, Schersten T. Skeletal muscle metabolism in patients with cancer. Europ J Cancer 1976; 12: 465–473.Google Scholar
  12. 12.
    Moldawer LL, O'Keefe SJD, Bothe A, Bistrian BR, Blackburn GL.In vivo demonstration of the nitrogen sparing mechanisms for glucose and amino acids in the injured rat. Metabolism 1980; 29: 173–180.PubMedCrossRefGoogle Scholar
  13. 13.
    Lundholm K, Edstrum S, Ekman L, Schersten T. Evaluation of anorexia as the cause of altered protein synthesis in skeletal muscle from nongrowing mice with sarcoma. Cancer Res 1981; 41: 1989–1996.PubMedGoogle Scholar
  14. 14.
    Stein TP, Oram-Smith JC, Leskow MJ, Wallace HW, Miller EE. Tumor caused changes in host protein synthesis under different dietary situations. Cancer Res 1976; 36: 3936–3940.PubMedGoogle Scholar
  15. 15.
    Heddon MR, Buse MG. General stimulation of muscle protein synthesis by branched chain amino acidsin vitro. Proc Soc Exp Biol Med 1979; 160: 410–415.Google Scholar
  16. 16.
    Schaur RJ, Semmelrock HJ, Schrubmayer W, Tilliam HM, Schavenstein E. Tumor host relations: nitrogen metabolism in Yoshida sarcoma-bearing rats, reduction of growth rate and increase of survival time by administration of physiologic doses of branched chain amino acids. J Cancer Res Clin Oncol 1980; 97: 285–293.PubMedCrossRefGoogle Scholar

Copyright information

© The Japan Surgical Society 1985

Authors and Affiliations

  • Isao Kawamura
    • 1
  • Hiroshi Sato
    • 1
  • Shohei Ogoshi
    • 2
  • George L. Blackburn
    • 3
  1. 1.Department of Surgery 2Chiba University School of MedicineChibaJapan
  2. 2.Department of Surgery 2Kochi Medical SchoolKochiJapan
  3. 3.Department of SurgeryHarvard Medical SchoolBostonU.S.A.

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